Investigation of the release of growth factors from apheresis platelet concentrate (APC) loaded three layered composite nanofiber surface

Abstract

In this study, a nanofiber surface loaded with apheresis platelet concentrate (APC) was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface (n=5) consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods (p<0.05). When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group (p>0.05), but there was a significant difference between the EGF and PDGF-AA growth factors (p<0.05).