Journal of Biological and Environmental Sciences

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Acute toxicity of abamectin (Insecticide) on Daphnia magna (Straus, 1820)
(Uludağ Üniversitesi, 2016-12-16) Azgın, Cansev; Göksu, Münir Ziya Lugal
Abamectin is used for the insect and mite pests control cotton, citrus fruit, vegetables, potatoes and other crops. Abamectin is not directly used in aquatic ecosystems, but it may have adverse impact on aquatic environment. The aim of this study was to determine the acute toxicity of abamectin (insecticide) on Daphnia magna (Straus, 1820) (Cladocera, Crustacea). D. magna were obtained from the Çukurova University Fisheries Faculty Freshwater Fish Research and Application Station. In the experiment, five different concentrations and one control group have been used. Each experiment was repeated two times. In this research, the static test method of acute toxicity test was used. The experiments were conducted under laboratory conditions at 20±2 oC. The results have been estimated with the dose-response data and were fitted with a log-logistic model by using R 3.0 statistical computation environment and DRC library. The 24-h and 48-h acute LC50 values were determined. According to the results, acute toxic effects researched Abamectin, the 24 h and 48 h LC50 acute toxic lethal concentration values for D. magna were calculated to be 0.020 µgl-1 and 0.0043 µgl-1 .
Acute toxicity of bentazone (herbicide) and chlorpyrifos (insecticide) on oreochromis niloticus (L., 1754) larvae (bioassay)
(Uludağ Üniversitesi, 2015-01-12) Azgın, Cansev; Göksu, Münir Ziya Lugal
In this study, in aquatic environments negative effects which may occur in order to have information about, the acute toxicity Bentazone (Herbicide) and Chlorpyrifos (Insecticide) which widely used in agriculture in Cukurova Region has been researched into larvae Oreochromis niloticus (L., 1754). In this study, the test fish O. niloticus larvae (average weight 1.21±0.52 g, average length 4.29±0.2 cm) were obtained from the Çukurova University Fisheries Faculty Freshwater Fish Research and Application Station. In the experiment, five different concentrations together with the control group were used 2 test series. In the present study, methods of bioassay test which static method has been applied. The study was carried out under laboratory conditions at 25±2 oC. The results has been estimated with the dose-response data were fitted a log-logistic model by using R 3.0 statistical computation environment and DRC library. The 24-h acute LC50 values were calculated. According to the results, acute toxic effects researched Bentazone (Herbicide) and Chlorpyrifos (Insecticide) the 24-h LC50 acute toxic lethal concentration values for Oreochromis niloticus (L., 1754) larvae were calculated Bentazone (Herbicide) LC50 14.86±0.19 mgl-1 and Chlorpyrifos (Insecticide) LC50 0.40±0.02 mgl-1.
Adiponectin and its protective effects
(Uludağ Üniversitesi, 2012) Guzel, Saime; Yalcin, Abdullah; Veteriner Fakültesi
Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is one of the adipocytokines that is secreted by the white adipose tissue and is an important regulator of lipid and glucose metabolism. It is an insulin-sensitizing hormone with anti-diabetic, anti-inflammatory, anti-atherogenic and anti-proliferative properties. It was demonstrated that decreased serum adiponectin levels are associated with insulin resistance and type 2 diabetes. Furthermore, hypoadiponectinemia was shown to be associated with coronary artery disease. Several authors point out that high levels of circulating adiponectin reduce risk of coronary heart disease among type 2 diabetes patients. Adiponectin has also been shown to play an important role in the regulation of cell proliferation and tumorigenesis. Adiponectin, via its cognate receptors AdipoR1 and AdipoR2, inhibits cancer cell proliferation in vitro and supressess obesity-associated tumor growth in vivo (e.g. colorectal, gastric, liver, and breast cancer). Therefore, potential diagnostic and therapeutic usage of adiponectin has been subject of an increasing interest in recent years.
Adzuki beans (Vigna angularis), a traditional Chinese legume for sustainable agriculture and food production
(Bursa Uludağ Üniversitesi, 2019-06-12) Shahrajabian, Mohamad Hesam; Sun, Wenli; Khoshkharam, Mehdi; Zandi, Peiman; Cheng, Qi
Adzuki bean (Vigna angularis) is a traditional legume crop that plays a crucial role in the sustainability of both agricultural systems and in the food protein supply. As adzuki bean has been used for thousands of years in China as traditional Chinese medicine and foods, it has a great potential to be drug candidate or functional food materials. Adzuki is a good source of essential fatty acids, fiber, minerals and phytochemicals such as polyphenols and phytates. Legumes are nitrogen-fixing systems that have long been used for biological nitrogen fixation in agriculture. Biologically fixed nitrogen of adzuki beans can be yielded so that the hazards of the chemical fertilizers can be reduced. Because of the growing requests for plant production, especially protein and oils and also decrease the economic and environmental pressure on agro-eco systems, it emerges that grain legumes, adzuki bean included would play a major role in future cropping systems and sustainable agriculture. Legumes, especially those which have various medicinal effects can help to restore soil organic matter and reduce pests and diseases problems, and they may lead to more soil fertility. Adzuki beans have considerable potential globally to be a functional food for health promotion and diseases prevention in not only Asian countries, but also other parts of the world.
Allelopathy of heliotropium europaeum (Boraginaceae): Influence on small grain cereals
(Uludağ Üniversitesi, 2016-10-07) Aliloo, Ali Asghar
In this research, probable allelopathic effects of Heliotrope were investigated on early growth of wheat and barley. Aqueous extracts of the plant at 5, 10, 15 and 20% concentrations were prepared from whole dried plants and distilled water was used as control. Results indicated that released allelochemicals from Heliotrope had inhibitory effects on germination and seedling growth of the test seeds when compared with the control. With increasing aqueous concentrations the inhibitory effects were severed and the highest concentration (20%) had maximum inhibitions on both traits. The results also revealed that the sensitivity of the plants to allelochemicals was different. Wheat was more tolerant than barley to the materials. The results also showed that the root growth was more susceptible than shoot growth under chemical stresses. Allelochemicals changed seed reserve remobilization rates. The weight of reserve mobilization and seed reserve depletion percentage decreased while seed reserve utilization efficiency improved. These findings suggest that the catabolism reactions are more susceptible to allelochemicals than anabolic reactions. It was concluded that Heliotrope had a powerful allelopathic effect on the early growth of the crop plants and the interference of Heliotrope was a species-specific phenomenon.
Allergenic fungal spore concentrations in the atmosphere of Bursa, Turkey
(Uludağ Üniversitesi, 2007) Ataygül, Elif; Celenk, Sevcan; Canıtez, Yakup; Bıcakcı, Adem; Malyer, Hulusi; Sapan, Nihat; Tıp Fakültesi; Biyoloji Bölümü
The daily distribution of allergic fungal spores for Bursa during 1999 was established using a 7-day volumetric Lanzoni trap. Ten of the important allergic fungal spores (Cladosporium sp., Alternaria sp., Aspergillus sp. / Penicillum sp., Fusarium sp., Epicoccum sp., Drechslera sp., Pithomyces sp., Stemphylium sp., Chaetomium sp. and Curvularia sp.) were identified. The dominant spore types detected were Cladosporium 88.11%, Alternaria 4.99%, Aspergillus / Penicillum 4.65%., Fusarium 0.84%, Epicoccum 0.62%, Drechslera 0.24%, Pithomyces 0.15%, Stemphylium 0.14%, Chaetomium 0.13% and Curvularia 0.13%. Spore levels of Cladosporium, Alternaria and Aspergillus / Penicillum peaked throughout the year. Fusarium April- June, Epicoccum June-August, Dreschlera July, September, Pithomycetes March, June and August, Stemphylium June-July, Chatemium May, July, Curvularia peaked July. In conclusion, a range of allergenic fungal spores were present in the air of Bursa throughout the year.
Analysis by scanning electron microscopy of polyethylene terephthalate and nylon biodegradation abilities of Bacillus sp. strains isolated from soil
(Bursa Uludağ Üniversitesi, 2020-12-07) Demirkan, Elif; Güler, Baran Enes; Sevgi, Tuba; Fen Edebiyat Fakültesi; Biyoloji Bölümü
Plastic pollution, the aggregation of synthetic plastic products in the environment generates serious problems for wildlife, habitats and the human population. Plastic accumulates at ocean, creating areas called the seventh continent, a mass of plastic garbage. Plastic wastes are disposed of by recycling, burying and incineration. However, these also have disadvantages. They are developing bioplastics as new solutions to plastic problems. Other alternative solutions may be microorganisms. Compared to other conventional technologies, it is extremely inexpensive and efficient in terms of cost and simplicity because it is based on the capabilities of microorganisms. In this study, we have reported the degradation abilities of B. subtilis and B. cereus strains on polyethylene terephthalate (PET, water bottle) and nylon (plastic bag). In the nylon medium with Bacillus subtilis ET18 and Bacillus cereus ET30 was observed red pigment formation. All bacteria showed biofilm formation in the presence of nylon. The surface morphology changes of the PET and nylon were determined by light microscope and SEM. The bacteria used in this study were found to biodegrade nylon more easily than PET. The activities of lipase, protease and α-amylase were determined. Among the enzymes, lipase was detected in the presence of both PET and nylon.
Anti-Aflatoxigenc potentials of two Nigerian herbs on albino rats
(Uludağ Üniversitesi, 2009) Fapohunda, Stephen Oyedele; Akintewe, Tosin; Olarinmoye, Ayodefi; Ezekiel, Chinbudu Ngozi
The anti-aflatoxigenic activity of methanolic and ethanolic extracts of Phyllanthus niruri and Pseudocedrella kotschyi were studied in male and female Wister albino rats fed with aflatoxin –contaminated chow (112ppb) for 20 d. Morbidity studies arising from the dietary aflatoxin were carried out. Packed Cell Volume (PCV), fasting blood sugar (FBS), haemoglobin concentration (HB), total white blood cells (WBC), and serum bilirubin (SB) were profiled.. Extracts from both herbs lowered the PCB, blood glucose, Hb, WBC and SB levels in the test male than in control at P<0.05 using the ANOVA test for significance. The ethanolic extracts of Pseudocedrella and the methanolic extract of Phyllanthus were the most effective anti-aflatoxigenic agents in the male as they reduced the blood parameter levels by 50% of the positive control. The results were not so in the females. Post-mortem examination revealed kidney discoloration and paleness, mild to moderate hepatomegaly and enlarged spleen in the positive control while test rats showed normal morphology for these organs. The 2 local herbs are good candidates for the management of aflatoxicosis in mammals.
Antifungal efficiency of biological control agents against phytopathogenic fungi
(Uludağ Üniversitesi, 2016-10-01) Şehirli, Sercan; Saydam, Cansu; Ziraat Fakültesi; Bitki Koruma Bölümü
In the present study, six different biological control agents were used against phytopathogenic fungi to determine the antifungal activities. The aim of this study was to assess the in vitro activity of Trichoderma harzianum, T. harzianum-T. viride, Aureobasidium pullulans, Lactobacillus acidophilus and Bacillus subtilis. These bio control agents were used against phytopathogenic fungi such as Fusarium culmorum, F. oxysporum, Sclerotinia sclerotiorum, Rhizoctonia solani and Macrophomina phaseolina, respectively. In vitro antagonism screening was carried out to test six antagonists against phytopathogenic fungi. Assessment of phytopathogenic fungi mycelial growth was done in 7 days at 25oC after its placement on the culture medium. The all antagonists were shown high inhibition on fungi mycelial growth. The most effective antagonist against fungi was T. harzianum and efficiency rate were continued with Bacillus subtilis, Aureobasidium pullulans and Lactobacillus acidophilus, respectively. Subsequently, in the second phase of in vitro, Captan, Maneb and Thiram were used to determine efficiency on fungi’s culture and survivability of the biocontrol agents. Fungicide dose was selected in label for each one and was used half, recommended and double concentrations. The most resistant plant pathogens against fungicides were M. phaseolina and F. oxysporum, on the other hand, most sensitive were R. solani and S. sclerotiorum when compare to all.
Aquatic macrophytes and soil features on Karamik lake coastal ecosystem in Afyonkarahisar (Turkey)
(Uludağ Üniversitesi, 2014-12-23) Serteser, Ahmet; Acar, Hasan
Karamik Lake and marshlands is located in inner-west Anatolia and Afyonkarahisar boundaries, being in B3 square according to the grid system. Most of the study area is covered by Quaternary lands. Bioclimate of the area is a combination of little rainy - cold Mediterranean climates. The precipitation regime shows the characteristic of eastern Mediterranean. Most of the study area has alluvial soils, hydromorphic alluvial and colluvial soils with dunes and marshes of land type. Floristic region of the area is a IranoTuranian type and about 50 vascular plant types have been identified. 6 plant communities are identified regarding to BraunBlanquet(1932) in the research area. These are Lycopus europaeus, Tetragonolobus maritimus, Scirpoides holoschoenus, Schoenoplectus litoralis, Sparganium erectum subsp. microcarpum, Carex distans. To accomplish the research the communities of plant have been pulled up with their soil and the analysed and evaluated. To find the relationship with this plantcommunities 0-30 and 30-60 cm soil samples retrieved from the depths and their physical-chemical analyses and commented.
Assessment of aqua crop model in the simulation of potato yield and water use efficiency under different water regimes
(Uludağ Üniversitesi, 2014-07-07) Afshar, Atefeh; Afsharmanesh, Gholam Reza; Adeli, Mohsen; Malekian, Ashraf
Conducting field experiments to determine the optimum amount of consumption water and achieve the optimal yield of plants is costly and time-consuming. Therefore, the aquacrop computer model was used to determine crops yield. The present study evaluates the performance of AquaCrop, a crop simulation model developed by FAO, in simulating potato (Solanum tuberosum L.) yield and water use efficiency under different water regimes in Jiroft region, Iran. Three irrigation levels, 100%, 75% and 50 % of water requirement, were arranged as a stripe-plot arrangement based on randomized block. The AquaCrop model revealed that potato yield was increased with increasing water consumption. The simulated potato yield was lower than measured potato yield in 100% and 70% of potato requirement scenarios but not in 50% conditions. The highest water use efficiency in the field conditions occurred under 75% of water requirement but the highest water use efficiency predicted by AquaCrop was found to be under 100% water requirement. Overall, results showed that the AquaCrop model could predict relatively good tuber yield, water use efficiency and water requirement values of potato under Jiroft conditions
Assessment of some pollution parameters with geographic information system (GIS) in sediment samples of Lake Uluabat, Turkey
(Uludağ Üniversitesi, 2014-04-08) İleri, Saadet; Karaer, Feza; Katip, Aslıhan; Onur, S. Sonay; Aksoy, Ertuğrul; Ziraat Fakültesi; Toprak Bölümü
The scope of work is to determine the quality of the lake Uluabat sediment. pH, electrical conductivity (EC), orthophosphate phosphorus (PO4-P), total phosphorus (TP), ammonium nitrogen (NH4-N), nitrate nitrogen (NO3-N), total nitrogen (TN), organic matter, and water content were monitored from June 2008 to May 2009 monthly with the samples taken from 8 different stations in the lake. Additionally sediment quality parameters were mapped to the ArcGIS 9.1 software and distribution of pollution were visualized with pollution distribution maps. As a result, in the stations 1., 5., 7., 8. have been found to be more intense pollution load when these maps were examined.
Asymptomatic prostatomegaly in a young anatolian shepherd dog
(Uludağ Üniversitesi, 2015-04-07) Çeçen, Göksen; Goncagül, Gülşen; Kurt, Hasan; Mennan Pasinler Meslek Yüksekokulu; Cerrahi Ana Bilim Dalı
This case was aimed to report clinical, radiological, ultrasonographical and bacterial culture analysis results of a young dog with prostatomegaly. An 18-month-old age Anatolian shepherd dog undergoing treatment of chronic non-healing pressure wounds in hind leg was presented with a small amount of liquid stool and prolonged straining to defecate. Prostatomegaly was diagnosed radiographically and the other examinations confirmed the pathology. Chronic prostatitis caused by prostatomegaly had developed insidiously without prior bouts of acut prostatitis in this dog. The case was treated succesfully.
Availability, accumulation and elimination of cadmium by artemia urmiana in different salinities
(Uludağ Üniversitesi, 2010) Rahimi, Bita; Manavi, Parisa Nejatkhah
The effect of salinity on cadmium uptake and elimination by brine shrimp Artemia urmiana which lives only in Urumieh Lake, Iran, was studied for the first time. Brine Shrimp were acclimated to different salinities and exposed to the metal in solution at differing salinities and composition. Experimental salinities ranged from 50 to 125 gL-1 and the concentrations for cadmium added to the solution were 20 and 5 mg/L. At first, bioaccumulations of Cd (5, 10, 15 and 20 mg/L Cd) were measured on day 1, 5, 11 and 17 of life. The results showed that, accumulation of cadmium in A. urmiana, depends upon the concentration of cadmium in the environment and increased with time. It also increased with the concentration of Cd in the environment. Maximum accumulation of Cd (0.748 mg kg -1) was observed with 20 mg/L Cd and on the 11th day of life of Artemia. Within each acclimation group, the cadmium uptake decreased with increasing exposure to salinity. To study cadmium elimination, samples were taking at 0, 6, 12, 24, 48, and 72 h. Results showed that, Artemia urmiana has the ability to accumulate and eliminate cadmium. Accumulation and also elimination of Cd by Artemia urmiana in 20 mg/L Cd were higher than in 5 mg/L Cd in all studied salinities. The cadmium accumulation pattern which seems well conserved in Artemia is characterized by a fast elimination of the metal.
Bio-Monitoring of heavy metal resistance in pseudomonas and pseudomonas related genus
(Uludağ Üniversitesi, 2012) Ceylan, Özgür; Uğur, Aysel
The aim of present study was to determine the level of heavy metal resistance patterns and to determine if there is a relationship between heavy metal resistance and plasmid presence. From 28 identified strains, 39.3% corresponded to Stenotrophomonas maltophilia, 17.9% to Chryseomonas luteola, 14.3% to Pseudomonas fluorescens, 10.7% to Pseudomonas putida, 7.1% to Sphingomonas paucimobilis, 7.1% to Methylobacterium mesophilicum and 3.6% Pseudomonas stutzeri. The resistance of these Gram negative bacteria to 8 heavy metals, was investigated by agar dilution method. Most isolates showed tolerance to different concentrations of heavy metals, and minimal inhibition concentrations ranged from 0,005 mmol-1 -20 mmol-1 . All strains displayed high resistance to zinc and lead (100% and 96,4% respectively) and high susceptibility to silver, cobalt and mercury (92.9%, 92.9% and 96.4% respectively). M. mesophilicum strains were determined as the most resistant strains to studied heavy metals. Isolated bacteria were tested for the presence of plasmids using the modified hot alkaline lysis method. The study also demonstrated that about 17.8% of isolated bacteria carried 0.89-21.59 kb sized plasmids and metal resistance profiles of bacteria carrying the same plasmids were similar. This study reveals the heavy metal resistance profiles of non-aeruginosa Pseudomonas species and other related species and the association between the occurrence of plasmids and the resistance to heavy metals.
Biochemical alterations induced by sublethal cyanide exposure in common carp (Cyprinus carpio)
(Uludağ Üniversitesi, 2013-06-25) Sadati, Fahimeh; Shahsavani, Davar; Baghshani, Hasan
The present study was designed to study the effects of sublethal cyanide exposure on some blood biochemical parameters in common carp. Three groups of fish (25 in each group) were treated with different concentrations of 0 (control), 0.1 or 0.2 mg/L potassium cyanide (KCN) for 2 weeks. At the end of each exposure, blood samples were collected and serum biochemical parameters were measured using validated methods. Cyanide exposure at both concentrations caused significant (P<0.05) elevation of aspartate aminotransferase and lactate dehydrogenase activities and creatinine concentration as compared to control group. Indeed, alanine aminotransferase, alkaline phosphatase and glucose levels in the carp treated with 0.2 mg/L KCN were significantly higher than those levels from controls. On the other hand, other measured biochemical parameters including gamma-glutamyl transferase, triglyceride, cholesterol, total protein, and albumin did not change significantly following cyanide exposure. The observed alterations in some measured serum biochemical parameters would reflect tissue damages, especially in liver and kidney, due to chronic cyanide intoxication in common carp and may be used for better understanding the pathophysiology of this toxicity and as an aid in the diagnosis of cyanide poisoning in fish species.
Biochemical changes and SDS-PAGE analyses of chickpea (cicer arietinum L.) genotypes in response to salinity during the early stages of seedling growth
(Uludağ Üniversitesi, 2014-07-04) Arefian, Mohammad; Vessal, Saeedreza; Bagheri, Abdolreza
Salinity is one of the most serious abiotic stresses for plants, causing other subsequent consequences such as oxidative stress, which eventually leads to cell death. Measured various biochemical parameters in chickpea genotypes were performed under various NaCl concentrations (0, 8 and 12 dS.m-1 ( in controlled condition at 21 and 28-day after sowing (DAS). After determination of tolerant (MCC544) and susceptible (MCC806) genotypes and also the best differential salt concentration, SDS-PAGE was used to compare protein profiling in these two genotypes in 3 time points with four replicates. Proline and protein contents were significantly higher in MCC544 as much as 27 fold (for proline) and 30% (for protein) increase over control in 28 DAS at 12 dS.m-1 of salt. The leaf soluble carbohydrates increased significantly in MCC544 and MCC760, compared with others. The minimum decline of electrolyte leakages (6%) and malondialdehyde (MDA) content was belonged to MCC760 while MCC806 genotype showed the highest decrease rate (more than 20%). Total leaf chlorophyll content decreased in all genotypes. More strong and positive correlations between parameters was recorded in tolerant genotypes which resulted in membrane and osmotic balance. Analyses of SDS-PAGE revealed that more rapid accumulation and/or less degradation of proteins occurred in higher molecular weight proteins. Moreover, the response of genotypes through protein changes before 96 h stress might be a possible reason for salinity tolerance in this condition.
Biodegredation of different agro-endustrial wastes through the cultivation of pleurotus ostreatus (Jacq. ex. Fr) Kummer
(Uludağ Üniversitesi, 2017-03-03) Atila, Funda
The aim of this study was to investigate the effect of various substrates on spawn running time, yield and biological efficiency (BE) of Pleurotus ostreatus (Jacq. ex. Fr) , compositional changes of substrates after growth mushroom and to evaluate its potential use as a feedstock. In the study, chickpea straw (CS) was used as basal substrate (80 %), while some of agricultural wastes such as cotton seed hulls (CSH), olive press cake (OPC), sunflower seed press cake (SPC) and sugar beet pulp (SBP) were added at the rate of 20 % to basal substrate. It was determinated that all of agricultural wastes tested in the study, except SBP, are economically viable alternatives for P. ostreatus (Jacq. ex. Fr) growers. On the other hand, the increment in ash, N, P, K, Mg, Fe and Mn content and the decrease in moisture, pH, C, C:N, Zn and Cu, cellulose, hemicellulose and lignin content of substrates were observed after mushroom production. According to this results, we can suggest that CSH, OPC and SPC can be used as a additive material for P. ostreatus (Jacq. ex. Fr) cultivation and spent mushroom substrates may be a valuable material as a feedstock, because of higher nutritional content and digestibility.
Biogas potential in Görükle campus of Uludağ University
(Uludağ Üniversitesi, 2016-10-08) Alibaş, İlknur; Erdoğan, Hilal; Yılmaz, Aslıhan; Alibaş, Kamil; Fen Bilimleri Enstitüsü; Biyosistem Mühendisliği Bölümü
In this study, the potential of animal manure of the Application Research Centres of the Faculty of Agriculture and the Ranch of Veterinary Medicine and located within the boundaries of Görükle Campus at Uludağ University, the waste of rapeseed, sunflower, and wheat production of the Agricultural Research and Application Centre at the Agricultural Faculty and food waste of all dining halls, restaurants and cafeterias, particularly the Central Dining Hall, situated on the campus was determined in order to determine the biogas potential of the campus. As the dry matter, based on the organic waste potential, the biogas potential relating of the campus was calculated to be 499962.91 m 3 . It was determined that 17.95% of this potential consisted of animal manure, 46.15% of it consisted of agricultural production waste and 35.90% of it consisted of food waste. It was calculated that the electric energy potential obtained by transforming the biogas potential into electric energy by means of a generator was 980.22 MWh.
Biological denitrification using pure cultures isolated from wastewater treatment plant of Khenchela (Eastern Algeria)
(Uludağ Üniversitesi, 2015-04-17) Naili, Oumaima; Benounis, Messaoud; Darbouche, Abdelhak; Kheddouma, Asma
The purpose of this study was to investigate biological dentirification of wastewater by pure cultures isolated from activated sludge of wastewater treatment plant of Khenchela (Eastern Algeria). Experiments were performed in batch reactor under anaerobic conditions. Three pure strains were isolated and identified as Enterobacter cloacae, Pseudomonas luteola and Aeromonas hydrophila Three carbon sources were used in this work; glucose, methanol and lactose. High denitrification was obtained at 2000 ppm for different carbon sources. Kinetic of bacterial growth and denitrification were studied. We show that bacterial growth rate directly influences nitrate removal from the medium and the use of glucose as carbon source provide high denitrification rate than methanol and lactose. These strains isolated from activated sludge will be used to develop new biosensors applied to the detection and determination of nitrate in real water.

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