Acute toxicity of bentazone (herbicide) and chlorpyrifos (insecticide) on oreochromis niloticus (L., 1754) larvae (bioassay)

Abstract

In this study, in aquatic environments negative effects which may occur in order to have information about, the acute toxicity Bentazone (Herbicide) and Chlorpyrifos (Insecticide) which widely used in agriculture in Cukurova Region has been researched into larvae Oreochromis niloticus (L., 1754). In this study, the test fish O. niloticus larvae (average weight 1.21±0.52 g, average length 4.29±0.2 cm) were obtained from the Çukurova University Fisheries Faculty Freshwater Fish Research and Application Station. In the experiment, five different concentrations together with the control group were used 2 test series. In the present study, methods of bioassay test which static method has been applied. The study was carried out under laboratory conditions at 25±2 oC. The results has been estimated with the dose-response data were fitted a log-logistic model by using R 3.0 statistical computation environment and DRC library. The 24-h acute LC50 values were calculated. According to the results, acute toxic effects researched Bentazone (Herbicide) and Chlorpyrifos (Insecticide) the 24-h LC50 acute toxic lethal concentration values for Oreochromis niloticus (L., 1754) larvae were calculated Bentazone (Herbicide) LC50 14.86±0.19 mgl-1 and Chlorpyrifos (Insecticide) LC50 0.40±0.02 mgl-1.Bu çalışmada, sucul ortamlarda meydana getirebileceği olası olumsuz etkiler hakkında bilgi sahibi olabilmek için, Çukurova Bölgesi’nde tarım alanlarında yoğun olarak kullanılan Bentazone (Herbisit) ve Chlorpyrifos (İnsektisit)’ un Oreochromis niloticus (L.,1754) yavruları üzerine akut toksik etkisi biyodeney yöntemiyle araştırılmıştır. Çalışma, laboratuarda 25±2oC’de, akvaryumlarda, ortalama ağırlığı 1.21±0.52 g ve ortalama boyu 4.29±0.2cm olan balıklarla, her pestisit için 2’şer tekrarlı, statik biyodeney yöntemine göre yürütülmüş; log-logistik model istatistik analiz yöntemiyle incelenmiş ve 24 saatlik akut LC50 hesaplanmıştır. Araştırma sonucunda, Oreochromis niloticus (L.,1754) yavrularına ait 24 saatlik akut toksik öldürücü konsantrasyon değerleri, bir herbisit olan Bentazone için LC50 14.86±0.19 mgl-1 ve bir insektisit olan Chlorpyrifos için LC50 0.40±0.02 mgl-1 olarak hesaplanmıştır.