Browsing by Author "Cansev, Asuman"
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Item The activities of catalase and ascorbate peroxidase in olive (Olea europaea L. cv. Gemlik) under low temperature stress(Korean Soc Horticultural Science, 2011-04) Cansev, Asuman; Gülen, Hatice; Eriş, Atilla; Uludağ Üniversitesi/Veterinerlik Fakültesi/Bahçe Bitkileri Anabilim Dalı.; 0000-0002-3353-846X; AAH-4255-2019; 26326677200; 6603211102; 6602612385In this study, one-year-old shoots of the olive (Olea europaea L.) cv. Gemlik were tested at artificial low temperatures (4, -5A degrees C, -10A degrees C, and -20A degrees C) every month for two years. For low temperature treatment, the degree of cell membrane injury in leaves and barks was determined by ion leakage method. In addition, with regard to antioxidative defense mechanism, activities of catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11) enzymes were determined. Leaf and bark tissues subjected to 4A degrees C and -5A degrees C injured to a limited extent in all months. However, more than 50% injury occurred by temperatures equal to or colder than -10A degrees C treatments depending on the season. For -10A degrees C and -20A degrees C treatments, the lowest and the highest injury in leaf and bark tissues were detected during winter and summer seasons, respectively. We determined in this study that CAT and APX enzyme activities are generally higher during fall and winter compared with those in summer. On the other hand, CAT and APX enzyme activities started increasing during fall along with a decreasing freezing injury while the activities of these enzymes decreased to some extent during winter when freezing injury was the lowest. In addition, while CAT activity decreased with low temperature treatments, APX activity did not change until -5A degrees C treatment but decreased with decreasing temperatures starting from -10A degrees C depending on the month the tissue was obtained. In conclusion, olive plant shows considerable tolerance to low temperatures that are achieved after daily gradual decreases by increasing cell membrane stability through complicated mechanisms including antioxidative enzyme metabolisms. In addition, APX may be more effective in maintaining cold-hardiness of olive compared with CAT.Item Anadolu adaçayı (Salvia fruticosa Mill.) bitkisinde denizyosunu uygulamalarının meydana getirdiği metabolik etkilerin belirlenmesi(Bursa Uludağ Üniversitesi, 2022-03-05) Yılmaz, Ruveyde Nur; Cansev, Asuman; Akpınar, Ayşegül; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.Biyolojik çeşitliliği zengin bir ülke olan Türkiye’ de tıbbi ve aromatik bitkiler önemli bir yere sahiptir. Anadolu adaçayı (Salvia fruticosa Mill.) ülkemizin kuzeybatısından güneybatısına kadar uzanan bölgede farklı lokasyonlarda yayılış gösteren ve ticari önemi olan bir türdür. İçerdiği etken maddelerden dolayı sağlık alanında kullanımının artması ve bitkinin yapraklarının çay olarak tüketilmesi Anadolu adaçayı bitkisinin popüleritesini gün geçtikçe arttırmaktadır. Deniz yosunları deniz çevrelerinde yetişen makro alglerdir. Deniz yosunu bünyesinde bulunan organik asitler ile makro ve mikro elementlerin ve çeşitli bitki büyüme düzenleyicilerinin varlığı nedeniyle, bitki destekleyici bir ürün olarak kullanım potansiyeline sahiptir. Bu nedenle son yıllarda deniz yosunu özütlerinin tarım ürünlerinin yetiştiriciliğinde kullanılması önem kazanmıştır fakat uygulama şekilleri ve dozlarının hangi bitkide hangi etkiyi yaratacağı henüz tam olarak belirlenmemiştir. Anadolu adaçayı türünde deniz yosunu uygulamasına ait literatürde herhangi bir çalışma mevcut değildir. Bu çalışmada Anadolu adaçayı (Salvia fruticosa Mill.) bitkisinde farklı dozlarda (0.25 g/L, 0.5 g/L, 1 g/L ve 2 g/L) deniz yosunu uygulamalarına bağlı olarak toplam fenolik madde içeriği, antioksidan kapasite (CUPRAC ve DPPH) ve antioksidatif enzim (SOD ve CAT) aktiviteleri ve glutatyon içeriğindeki değişimler incelenmiştir. Çalışmada, Anadolu adaçayı bitkilerindeki oksidatif yanıtlar ve toplam fenolik madde içeriği, antioksidan kapasite bakımından sonuçlar değerlendirildiğinde 1 g/L deniz yosunu özütünün uygulama dozu olarak uygun olabileceği belirlenmiştir. Bundan sonraki çalışmalarda, Anadolu adaçayı bitki türünde farklı deniz yosunu içeriklerinin etkileri, çeşitli dozlar ve uygulama şekilleri bakımından değerlendirilerek yeni çalışmalarla detaylandırılması önerilmiştirItem Annual patterns of total soluble sugars and proteins related to cold-hardiness in olive (Olea europaea L. 'Gemlik')(Taylor & Francis, 2007-07) Eriş, Atilla; Gülen, Hatice; Barut, Erdoğan; Cansev, Asuman; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAE-6913-2019; AAH-4255-2019; 6602612385; 35208237200; 26657823900; 26326677200Annual patterns of cold hardiness, total soluble sugars (TSS) and proteins (TSP) were characterised during two consecutive years (May 2003-April 2005) in leaf and bark tissues of olive (Olea europaea L.) 'Gemlik' trees. One-year-old shoots from 13-year-old olive trees were collected each month and exposed to low temperature at 4 degrees C, -5 degrees C, -10 degrees C, or -20 degrees C for 12 h to determine their cold-hardiness (LT50; assessed by electrolyte leakage). A considerable increase in cold-hardiness was observed with decreasing temperature. Leaf and bark tissues exhibited an increase in cold-hardiness during the Autumn, which reached a maximum in mid-Winter, then gradually decreased during the Spring, and reached a minimum in mid-Summer. Results indicated that leaf and bark tissues in both experimental years responded similarly to cold, with a continuous increase in their TSS and TSP contents during field adaptation in Autumn and Winter, which paralleled their cold-hardiness. The annual cycle of TSP from leaf and bark tissue was characterised by one dimensional SDS-PAGE. A seasonal fluctuation was observed in leaf proteins of 66 kDa and 43 kDa, and in bark proteins of 70 kDa, 43 kDa and 16 kDa, which paralleled cold-acclimation. Evaluation of these leaf and bark TSP profiles showed that these polypeptides disappeared in the Summer, followed by their accumulation again during the Autumn and Winter. Moreover, all these polypeptides became less visible during the Spring. The relationships between these five proteins, TSS contents, and cold-hardiness in olive are discussed.Publication Application of choline and glycine betaine alters levels of total phosholipids in olea europaea L. related to cold hardiness(Federation Amer, 2013-04-01) Cansev, Asuman; Gülen, Hatice; İpek, Meryem; Barut, Erdoğan; CANSEV, ASUMAN; Gülen, Hatice; İPEK, MERYEM; BARUT, ERDOĞAN; Uludağ Üniversitesi; 0000-0001-7586-3108; 0000-0002-3353-846X; AFW-5375-2022; AAH-4255-2019; AAE-6913-2019; AAH-3233-2021Publication Bioaccessibilities of K, P, Fe and B minerals in oleaster flour as a novel food ingredients(Federation Amer Soc Exp Biol, 2013-04-01) Şahan, Yasemin; Cansev, Asuman; Çelik, Güler; Göçmen, Duygu; ŞAHAN, YASEMİN; CANSEV, ASUMAN; GÖÇMEN, DUYGU; Uludağ Üniversitesi; 0000-0002-3353-846X; ABE-6748-2020; KDP-3344-2024; AAH-4255-2019Item Changes in antioxidant enzyme activities during cold-acclimation in sweet cherry cultivars grafted on different rootstocks(WFL Publication, 2013) Cansev, Asuman; Kesici, Müge; Uludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; ABD-6710-2020; 26326677200; 54412311700Plant acclimation to environmental stress is governed by several mechanisms including alterations in antioxidant defense systems. The purpose of this study was to investigate the effects of Gisela 5 and Mazzard rootstocks on the cold-hardiness of the sweet cherry cultivars (cvs.) 0900 Ziraat and Lambert, as well as to analyze the changes in activities of antioxidative enzymes including catalase (CAT; EC 1.11.1.6), soluble peroxidase (S-PRX; EC 1.11.1.7), cell wall-bound peroxidase (CWB-PRX; EC 1.11.1.7) and glutathione reductase (GR; EC 1.6.4.2) in cold-acclimated (CA) and non-acclimated (NA) stages. One-year shoots of 4 years-old sweet cherry tree cvs. 0900 Ziraat and Lambert grafted either on Gisela 5 or Mazzard rootstock were collected in CA (in January) or NA (in July) stage and subjected to artificial low temperature tests (4 degrees C, -5 degrees C, -15 degrees C and -25 degrees C) in order to determine the degree of cell membrane injury in bark tissues by ion leakage method. Antioxidant enzyme activities were assayed in another set of samples that were not subjected to low temperature treatments. Our data showed that ion leakage in bark tissues was greater in NA stage compared to CA stage. Ion leakage was below 50% in barks exposed to 4 degrees C and -5 degrees C in either period and above 50% in those exposed to -15 degrees C and -25 degrees C in NA stage. Ion leakage was lower if cultivars were grafted on Mazzard compared with grafting on Gisela 5. In addition, activities in both cultivars of CAT, as well as S-PRX and S-PRX, but not GR, were significantly higher during CA stage compared with those in NA stage. Elevations in CAT and PRX (both cell wall and soluble) activities were more prominent if the cultivars were grafted on Mazzard rootstock. We conclude that, cold hardiness in the sweet cherry involves changes in certain antioxidant enzyme activities depending usually on whether the plant is in CA or NA stage. Our finding that activities of CAT, as well as S-PRX and CWB-PRX in cultivars grafted on Mazzard were higher than those in cultivars grafted on Gisela 5 suggests that cold hardiness may be affected by the rootstock.Publication Characterization of phytochemical and functional properties of endemic edible Allium tuncelianum and comparison with commonly cultivated garlic, onion and leek crops(Springer, 2023-01-13) İPEK, MERYEM; ŞAHAN, YASEMİN; CANSEV, ASUMAN; Cansev, Asuman; İPEK, AHMET; Ipek, Ahmet; Altınseker Acun, Deniz Zahide; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; AAH-3233-2021; ABE-6748-2020; AAH-4255-2019; AAK-4655-2021Edible Allium tuncelianum ([Kollman] Ozhatay, Matthew and Siraneci), endemic to Turkey, has received increased attention in recent years because of its appealing characteristics, such as garlic (A. sativum L.)-like odor and single-clove solid bulb structure with few peels, which could be advantageous in the food processing industry. The aims of this study were to determine chemical composition, phytochemicals (alliin, allicin, phenolics), antioxidant capacity, and bioaccessibility of total phenolic content and antioxidant capacity in A. tuncelianum and to compare with the values in commonly cultivated Allium species: garlic, onion (A. cepa L.), and leek (A. porrum L.) to reveal its nutritional and health beneficial properties. The chemical composition of A. tuncelianum was comparable with that of garlic, and its dietary fiber content was three times higher than that of garlic. Alliin and allicin organosulfur compounds, which give garlic its distinct flavor and odor, were detected in all analyzed A. tuncelianum genotypes although their values lower than the amounts detected in garlic genotypes. However, alliin and allicin were not detected in any onion and leek genotypes. A. tuncelianum genotypes had higher total phenolic contents with over 78 percent bioaccessibility rates than garlic, onion, and leek. The antioxidant capacity of A. tuncelianum was comparable to those of cultivated Allium species. Our results suggested that A. tuncelianum can be considered a promising new health-enhancing crop for human nutrition.(GRAPHICS)Item Chemical and techno-functional properties of flours from peeled and unpeeled oleaster (Elaeagnus angustifolia L.)(Julius Kuhn Inst, 2015) Çelik, Güler; Dülger, Dilek; Kaplan, H. Betul; Sahan, Yasemin; Goçmen, Duygu; Cansev, Asuman; Aydın, Emine; Dundar, Ayse N.; Kılcı, Aslı; Gucer, Seref; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; Uludağ Üniversitesi/Keles Meslek Yüksekokulu/Gıda Teknolojisi Bölümü.; Uludağ Üniversitesi/Yenişehir Meslek Yüksekokulu/Gıda Teknolojisi Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0003-3457-251X; 0000-0001-6797-1985; 0000-0002-3353-846X; 0000-0003-2084-7076; ABE-6748-2020; CQO-7857-2022; AAH-4255-2019; DXI-0847-2022; AAA-3876-2022; FDE-3256-2022; GAV-3362-2022; 25629856600; 55967047900; 26326677200; 36150355600; 56895920400; 55860204000Oleaster flours were produced from two different genotypes (GO1 and GO2) and methods (peeled oleaster flour: POF and unpeeled oleaster flour: UPOF). Oleaster flour samples (OFs) contained high levels of dietary fibers and micro minerals. The contents of Fe, Cu, B, and Cr in flours obtained from oleaster fruits were higher in UPOF than in POF samples. Palmitic acid was the major fatty acid which was followed by oleic acid and lignoceric acid. All samples contained greater amount of saturated fatty acids (SFA) as compared to mono unsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Among seven different organic acids detected, the level of citric acid was the highest and it was followed by malic, acetic and oxalic acids. High nutritional contents of oleaster flour indicated that it is a good source of dietary fiber, micro minerals, as well as organic and fatty acids. The water solubilities (WS) and water absorption capacities (WAC) of oleaster flours were adequate for their utilization. They also seem to have an improving effect on emulsion properties of albumin. These results highlighted that it is possible to use the oleaster flour in some processed foods such as bakery goods, dairy products (ice cream and yoghurt), beverages and confectionery. Moreover, the oleaster flour could also be used in the preparation of low-fat, high-fiber dietetic products due to its high dietary fiber content.Item Chemical properties and antioxidant capacity of elaeagnus angustifolia L. fruits(Asian Journal of Chemistry, 2011-06) Çelik, Güler; Taşkesen, Selma; Cansev, Asuman; Şahan, Yasemin Bengü; Özbey, Hasan; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; ABE-6748-2020; 26326677200; 25629856600; 37114041500Elaeagnus angustifolia L. (oleaster) is an autumn fruit which is generally consumed dried. Although it grows almost everywhere in Turkey, use of its fruits is limited. We analyzed chemical composition and antioxidant properties of the edible parts of Elaeagnus angustifolia L. fruit. Total moisture, ash, protein, total soluble sugar, fat, crude fibre, total titratable acidity and major mineral content of the fruit were determined. Total polyphenols were investigated using Folin-Ciocalteau method and antioxidant capacity of the fruit was assessed by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) in the mesocarp and exocarp tissues. Results showed that potassium is the most abundant mineral in the fruit (8504 mg/kg) which is followed by sodium (1731 mg/kg) and phosphorus (635 mg/kg). Elaeagnus angustifolia L. fruit is a rich source of both several nutrients and antioxidant compounds. Average total phenolic content of aqueous, acetone and methanolic extracts of the mesocarp and exocarp in oleaster fruit were measured as 778 and 559, 390 and 361 and 414 and 524 mg gallic acid equivalents of 100 g(-1) dried mass, respectively. In conclusion, E. angustifolia fruit is a rich source of chemical compounds and has a high mineral content. This fruit can be included in dietary products due to its possible health benefits.Item Cold hardiness of olive (Olea Europaea L.) cultivars in cold-acclimated and non-acclimated stages: Seasonal alteration of soluble sugars and phospholipids(Cambridge Univ, 2009-08) Gülen, Hatice; Cansev, Asuman; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; 6603211102; 26326677200; 6602612385In many plant species, several physiological and biochemical changes occur during low-temperature-induced cold acclimation. A previous study with olive cultivars (Cansev et al. 2009) demonstrated a correlation between the level of accumulation of certain leaf proteins besides antioxidative enzyme activities and cold hardiness of the cultivars. The present paper analysed soluble sugar (SS) and phospholipid (PL) contents of cold-acclimated (CA) and non-acclimated (NA) leaf tissues in order to explain the mechanism of cultivar-dependent response to cold in olive. In general, cold acclimation significantly increased total soluble Sugar (TSS), reducing sugars and sucrose contents of all cultivars to various extents depending on the cold hardiness of cultivars. In addition, TSS, reducing sugars and sucrose contents in cold-tolerant cultivars were significantly increased, whereas TSS, reducing sugars and sucrose contents in cold-sensitive cultivars either did not change or increased slightly in CA stage compared with those in NA stage. Even though reducing sugars were the major soluble sugar in olive leaves, levels of sucrose accumulations in CA stage compared with those in NA stage were greater than those observed in reducing sugars accumulation. Changes in levels of total PL, as well as the three individual PL fractions phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), were investigated in olive leaf tissues. Significant increases in levels of PC and PE fractions during CA compared with those in NA Stage Suggested that PC and PE maintained the cold hardiness of olive cultivars more effectively than did PI. Although the precise mechanisms by which olive responds to cold may still be open to discussion', soluble sugars and PL are clearly important in the ability of olive cultivars to stand against cold stress.Item Cold-hardiness of olive (Olea europaea L.) cultivars in cold-acclimated and non-acclimated stages: Seasonal alteration of antioxidative enzymes and dehydrin-like proteins(Cambridge Univ, 2009-02) Cansev, Asuman; Gülen, Hatice; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; 26326677200; 6603211102; 6602612385Seasonal patterns of antioxidative enzymes and proteins and their relations to cold-hardiness of nine olive (Olea europaea L.) cultivars (Ascolona, Domat, Gemlik, Hojoblanca, Lecquest, Manzanilla, Meski, Samanli and Uslu) are documented in the current study. Fully expanded, uniformly sized leaves from 2-year-old Shoots Of the cultivars were collected from 20-year-old trees in cold-acclimated (CA, in January) and non-acclimated (NA, in July) stages. Leaf samples were exposed to low temperature at 4, -5, -10 and -20 C for 12 It to determine their cold-hardiness (LT50; assessed by electrolyte leakage). Cold-acclimation produced an increase in freezing tolerance of all cultivars (by lowering LT50). Domat and Lecquest were found to have the highest cold-hardiness among the nine cultivars investigated. Ascolona, Gemlik, Hojoblanca had moderate cold-hardiness, while Samanli, Meski, Uslu and Manzanilla were more sensitive. Activities of catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC 1.11.1.11) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase significantly varied depending on the cold-acclimation stage and the cold-hardiness level of the cultivars. Activities of the three antioxidative enzymes and total soluble proteins (TSP) were higher in the CA stage than in the NA stage. Although no accumulation or major polypeptides, except a 23 kDa protein, was detected either in CA samples or NA samples by SDS-PAGE, anti-dehydrin immunoblots revealed that the 43 and 23 kDa polypeptides were detectable during cold-acclimation of olive cultivars, Accumulation of both 43 and 23 kDa dehydrin was significantly higher in the CA stage than in the NA stage in all cultivars. Accumulation of 43 kDa dehydrin was correlated with cold-hardiness of the cultivars, while 23 kDa dehydrin was considered as cultivar-dependent since its accumulation was not parallel to LT50 values of the cultivars. Indeed, the tissues of cvs Domat, Lecquest, Ascolona, Hojoblanca and Gemlik were found to enhance the structural stability Of Cellular membranes in the CA stage by increasing both the activity Of Such enzymes as CAT, APX and NADPII oxidase to activate the antioxidative systems and the expression of 43 kDa dehydrins.Item Determination of non-phytotoxic doses of glycine betaine and choline in Olea europaea L.(Elsevier, 2012-09-23) Cansev, Asuman; Gülen, Hatice; İpek, Meryem; Barut, Erdoğan; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; AAE-6913-2019; AAH-3233-2021Item Determination of organophosphorus pesticide residues in olives grown in Bursa, Turkey(Int Soc Horticultural Science, 2011) Çelik, Güler; Çınar, Ahsen Sevde; Tous, J.; Gucci, R.; Cansev, Asuman; Şahan, Yasemin; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; ABE-6748-2020; 26326677200; 25629856600The use of pesticides in agriculture has been continuously increasing in recent years and has led to an increase in world food production. Organophosphorus compounds are among the most widely used pesticides in the world. Because of their long half-life time, they contaminate soils as well as surface and ground water. In addition, these residues in food constitute a significant health risk. Olive and its products are extensively consumed foods in the Mediterranean countries. However, olive trees are attacked by several pests and diseases, making it necessary to apply pesticides to ensure crop protection, which can leave residues on the fruit. Because pesticide residues in food constitute a significant health risk and olive oil has a high consumption rate among people of the producing countries, the continuous control of pesticide residues in olive oil is of great importance. In this study, the occurrence of nine organophosphorus pesticide residues in olives (Olea europaea L. 'Gemlik') in Mudanya and Gemlik towns (Bursa, Turkey; latitude: 29 degrees 04'E, longitude: 40 degrees 39'N) was investigated. Olive samples in the black maturation stage from twenty orchards were analyzed for pesticide residues using different extraction methods by capillary gas chromatography (GC) using NPD with a capillary column. Of the eight organophosphorus pesticides investigated, four were detected in olives namely azinphosethyl, chlorpyrifos, chlorpyrifos-methyl, and carbophenothion. The other four, diazinon, methidathion, malathion, and pirimiphos-methyl, were below detection limits. All samples contained more than one pesticide and 17 were found to contain chlorpyrifos at concentrations slightly above the maximum pesticide residue limit (100 mu g kg(-1), MRL).Item Development and validation of new SSR markers from expressed regions in the garlic genome(Univ Sao Paulo, 2015-01) Sahin, Nihan; Simon, Philipp W.; Ipek, Meryem; Ipek, Ahmet; Cansev, Asuman; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-0609-3442; 0000-0002-9136-3186; 0000-0002-3353-846X; AAH-3233-2021; AAK-4655-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was tested using 31 garlic genotypes. Twenty six EST-SSR markers amplified 130 polymorphic DNA fragments and the number of polymorphic alleles per SSR marker ranged from 2 to 13 with an average of 5 alleles. Observed heterozygosity and polymorphism information content (PIC) of the SSR markers were between 0.23 and 0.88, and 0.20 and 0.87, respectively. Twenty one out of the 31 garlic genotypes were analyzed in a previous study using AFLP markers and the garlic genotypes clustered together with AFLP markers were also grouped together with EST-SSR markers demonstrating high concordance between AFLP and EST-SSR marker systems and possible immediate application of EST-SSR markers for fingerprinting of garlic clones. EST-SSR markers could be used in genetic studies such as genetic mapping, association mapping, genetic diversity and comparison of the genomes of Allium species.Item Development of EST based SSR markers for garlic genome(Int Soc Horticultural Science, 2012) Simon, Philipp W.; Wako, T.; İpek, Meryem; İpek, Ahmet; Cansev, Asuman; Şeniz, Vedat; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-3233-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200; 13604787100Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.Item Domates pas akarı zararına karşı üridin ve akarisit uygulamalarının etkisi(Bursa Uludağ Üniversitesi, 2022-09-02) Atay, Özge; Cansev, Asuman; Kumral, Nabi Alper; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.; 0000-0003-1542-5960Domates (Solanum lycopersicum L.) bitkisi anavatanı Güney Amerika’nın batı kıyıları olan Solanaceae familyası türlerinden biridir. Sebze üretimi ve tüketimi içerisinde hem ülkemizde hem de dünya genelinde en çok üretilen ve tüketilen bitkiler arasında yer almaktadır. Domatesin pas akarına karşı toleransını arttıracak (zararın azalması ve hızlıca iyileşmesi ile bitki gelişimini teşvik etme gibi) alternatif çözümlere ihtiyaç duyulmaktadır. Bu çalışmayla birlikte, kontrollü koşullarda domates pas akarı bulaştırılan domates fidelerinde sadece üridin veya sadece akarisitlerin (abamectin, acequinocyl, pyridaben, spiromesifen) yarı dozlarının uygulaması ila bunların kombinasyonlarının akar zararına olan etkilerinin belirlenmesi amaçlanmıştır. Literatür taraması yapıldığında bu konuyla ilgili herhangi bir araştırmanın mevcut olmadığı gözlemlenmiştir. Denemede akarisit uygulanan kombinasyonlarda abamectin 0,25 mg/L, acequinocyl 62,5 mg/L, pyridaben 15 mg/L ve spiromesifen 25 mg/L konsantrasyonları kullanılmıştır. Bitkilerde büyüme oranı, klorofil miktarı, yaprak oransal su kapsamı ve turgor kaybı gibi fizyolojik parametreler ölçülmüştür. Bununla birlikte MDA, glutatyon ve askorbik asit miktarı ile antioksidatif savunma sistemine ait bazı enzimlerin CAT, APOX ve SOD aktivitesindeki değişimler de incelenmiştir. Çalışmada yapılan analizler sonucunda genel olarak tüm parametreler p<0,05 düzeyinde önemli bulunmuştur. Kontrol bitkilerine göre sadece üridin kullanılan bitkilerde tüm parametrelerde önemli düzeyde iyileşmeler tespit edilmiştir. Ayrıca, sadece akarisit kullanılan bitkilere kıyasla üridin kullanılan bitkilerin fizyolojik ve biyokimysal parametrelerinde önemli düzeyde iyileşmeler belirlenmiştir. Hem akarisit dozunun azaltılmasında hem de zararın düşürülmesinde üridinin etkisi olduğu tespit edilmiştir.Item Effect of processing techniques on antioxidative enzyme activities, antioxidant capacity, phenolic compounds, and fatty acids of table olives(Korean Society Food Science and Technology-Kosfost, 2013-01-08) Şahan, Yasemin; Cansev, Asuman; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0001-7586-3108; 0000-0002-3353-846X; AFW-5375-2022; AAH-4255-2019; ABE-6748-2020; 25629856600; 26326677200; 6603211102In this study, olive fruits (Olea europeae cv. Gemlik) of the most common sources of table olives in Turkey were used. Total polyphenol content (TPC), antioxidant capacity (AC), and antioxidant enzymes (catalase, CAT; ascorbate peroxidase, APX; and glutathione reductase, GR) of table olives were compared by 4 different methods of ripe table olive processing. Results revealed that TPC of the processed olives ranged from 117.44 to 418.69 mg gallic acid equivalents/g fresh weight (f.w.). The highest AC as mg Trolox equivalents of 189.58/g f.w. was obtained from unprocessed black olives. CAT, APX, and GR activities of unprocessed olives were higher than those obtained in all processed olives. In conclusion, TPC, AC, and antioxidant enzyme activities are strongly affected by fruit ripening and processing in table olives of 'Gemlik' cultivar. In addition, the best processing technique is untreated black olives in brine for antioxidant properties.Item Effects of different temperatures on some biochemical contents in garlic bulbs(American Society for Horticultural Science, 2016-09) İpek, Meryem; Cansev, Asuman; İpek, Ahmet; Koccat, Saime; Cansev, Mehmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; AAH-3233-2021; AAK-4655-2021; FEB-0069-2022; M-9071-2019Item Effects of high temperature stress on total phenolic content and antioxidant capacity in strawberry plants(Elsevier, 2012-09-23) Cansev, Asuman; Kesici, Müge; Ergin, Sergül; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; ABD-6710-2020; AAH-4255-2019; AAG-6558-2020Publication Effects of the lichen Peltigera canina on Cucurbita pepo spp. pepo grown in soil contaminated by DDTs(Springer Heidelberg, 2020-11-19) Akpınar, Ayşegül; Cansev, Asuman; İşleyen, Mehmet; CANSEV, ASUMAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019Lichens consisting of a symbiotic association of green algae or cyanobacteria and fungi are found in a variety of environmental conditions worldwide. Terricolous lichens, located in soils, affect the living and lifeless environment of the soil due to their effective secondary metabolite and enzymatic content. Terricolous lichens can increase the biological, chemical, and physical usefulness of soil. However, their effects in ensuring the bioavailability of contaminated soil are not known, especially on soil pollution caused by DDTs (p,p '-DDE, p,p '-DDD, p,p '-DDT). This research focuses on the effect of terricolous lichens on zucchini (Cucurbita pepo spp. pepo) grown in soil contaminated by DDTs, utilizing their secondary metabolite and enzymatic contents. Firstly, Peltigera canina, a terricolous lichen species, was added to soil contaminated by DDTs as powdered and intact thallus. After lichen addition to soil, zucchini was planted in. The oxidative stress and antioxidative enzyme activities of zucchini were measured. According to the results, P. canina treatments have a positive effect on the growth and development of zucchini, although oxidative stress was observed. Also, it was determined that powdered application had more effective results than intact thallus application.