Development of EST based SSR markers for garlic genome

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Date

2012

Journal Title

Journal ISSN

Volume Title

Publisher

Int Soc Horticultural Science

Abstract

Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.

Description

Bu çalışma, 21-24 May 2012 tarihleri arasında Fukuoka[Japonya]’da düzenlenen 6th International Symposium on Edible Alliaceae’da bildiri olarak sunulmuştur.

Keywords

Agriculture, Plant sciences, Allium sativum, Simple sequence repeats, Expressed sequence tag, Genetic mapping, Allium-sativum l., Genetic diversity, Rapd markers, Clones, Allium sativum

Citation

İpek, M. vd. (2012). "Development of EST based SSR markers for garlic genome". ed. T. Wako. Acta Horticulturae, VI International Symposium on Edible Alliaceae, 969, 81-83.