A phylogenetic and genotyping study of bovine herpesvirus type 4 (bhv-4) in Turkey
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Date
2020-05-26
Authors
Koç, Taylan
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Infertility and reproductive infections are the huge problems for the diary management throughout the world. Bovine herpesviruses act an enormous role in these complicated problems. Bovine Herpesvirus Type 1 (BHV-1) is the most outstanding herpesvirus causing genital and uterine tracts infections among other reproductive viral agents, however, Bovine Herpesvirus Type 4 (BHV-4) is also responsible in terms of similar symptoms and diseases. The main aims of the study are both to investigate the underlying potential presence of BHV-4 in subclinical uterine tract infection, and both to perform molecular and recombination analyses. A herd including 25 repeat breeder cows were investi-gated by BHV-4. Two out of them were determined BHV-4 infected after a series of Polymerase Chain Reactions (PCRs) tests which able to amplify partial Glycoprotein B (gB) and Thymidine Kinase (TK) gene regions. Obtained sequences were analyzed by using phylogenetic and recombinational software, and two Maximum Likelihood (ML) tree have been constructed. To results, novel Turkish BHV-4 sequences fell into Genotype I in both constructed Maximum Likelihood (ML) phylogenetic trees, however, no recombination evidence has been observed in relevant software. This report is one of the rare genotyping studies on BHV-4 from Turkey. This study showed that Turkish BHV-4 strains, as molecular, were highly probable to originate from European strains and might be observed in different clinical reflections. This suggests that BHV-4 should be deeply investigated by further molecular techniques and included in diagnostic panels for reproductive viruses.
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Keywords
Reproductive infection, Bovine herpesvirus type 4 (BHV-4), Repeat-breeder, Infertility, Genotyping, Turkey
Citation
Koç, B. T. (2020). "A phylogenetic and genotyping study of bovine herpesvirus type 4 (bhv-4) in Turkey". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 39(1), 61-66.