Anti-inflammatory efficiency of levobupivacaine in an experimental colitis model

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2010-05-28

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Baishideng Publishing Group

Abstract

AIM: To investigate the efficiency of levobupivacaine in treating experimentally induced colitis in rats. METHODS: Colitis was induced by trinitrobenzene sulfonic acid and ethanol in 30 rats under general anesthesia, and 10 rats were used as a sham group. Subsequent to induction of colitis, rats were divided into three groups; budesonide group received 0.1 mg/kg budesonide, levobupivacaine group received 10 mg/kg levobupivacaine and saline group received 1 mL saline solution via rectal route for 7 d. In the sham group, only routine rectal catheterization was performed without use of any material. At the end of 7 d, laparotomy and total colectomy were performed for histopathological examination in all rats and blood samples were drawn for measurement of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 following cardiac puncture. Macroscopic and microscopic evaluations of the specimens were performed by a pathologist blinded to group assignment of the rats. RESULTS: Weight loss (P = 0.016) and macroscopic examination scores (P = 0.001) were significantly higher in saline group than others. Histopathological scoring was comparable between all colitis groups (P = 0.350). There was no significant difference in TNF-alpha levels and IL-6 levels (P = 0.150). CONCLUSION: The significant improvement in macroscopic scores suggests that levobupivacaine may have topical anti-inflammatory effects in an experimental colitis model; however, this finding was not supported by microscopic findings.

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Keywords

Trinitrobenzene sulfonic acid, Colitis, Levobupivacaine, Budesonide, Inflammatory-bowel-disease, Ulcerative-colitis, Local-anesthetics, Lidocaine, Ropivacaine, Proctitis, Therapy, Cells, Gastroenterology & hepatology

Citation

Duman, U. vd. (2010). "Anti-inflammatory efficiency of levobupivacaine in an experimental colitis model". World Journal of Gastroenterology, 16(20), 2537-2541.