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Title: Development of EST based SSR markers for garlic genome
Authors: Simon, Philipp W.
Wako, T.
Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.
İpek, Meryem
İpek, Ahmet
Cansev, Asuman
Şeniz, Vedat
Keywords: Agriculture
Plant sciences
Allium sativum
Simple sequence repeats
Expressed sequence tag
Genetic mapping
Allium-sativum l.
Genetic diversity
Rapd markers
Allium sativum
Issue Date: 2012
Publisher: Int Soc Horticultural Science
Citation: İpek, M. vd. (2012). "Development of EST based SSR markers for garlic genome". ed. T. Wako. Acta Horticulturae, VI International Symposium on Edible Alliaceae, 969, 81-83.
Abstract: Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.
Description: Bu çalışma, 21-24 May 2012 tarihleri arasında Fukuoka[Japonya]’da düzenlenen 6th International Symposium on Edible Alliaceae’da bildiri olarak sunulmuştur.
ISBN: 978-90-66056-95-4
ISSN: 0567-7572
Appears in Collections:Scopus
Web of Science

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