Please use this identifier to cite or link to this item:
|Title:||Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts|
|Authors:||Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.|
Uludağ Üniversitesi/Tıp Fakültesi/Klinik Farmakoloji Anabilim Dalı.
Kahraman, Müjdat Müfit
Özyiğit, Musa Özgür
Broiler lung fibroblast
|Citation:||Akkoç, A. vd. (2011). ''Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts''. Avian Biology Research, 4(1), 6-16.|
|Abstract:||This study investigated the effects of hypoxic and hyperosmotic stresses on the matrix metalloproteinases 2 and 9 (MMP-2, MMP-9) and their tissue inhibitors 2 and 1 (TIMP-2, TIMP-1) respectively in cultured lung fibroblasts from broiler chickens. Isolated and sub-cultured primary lung fibroblasts from 1-day-old broilers were exposed to hypoxia and hyperosmosis. The presence of the enzymes and the effects of hypoxia and hyperosmosis on the enzymes in cultured lung fibroblasts, and in the culture media, were evaluated with the streptavidin-biotin-peroxidase method and western blotting using commercially available primary monoclonal antibodies. Short (6 hours) and long term (12 hours) hypoxia, hypoxia + hyperosmosis (6 hours) and hyperosmosis (6 hours) triggered the release of both MMP-2 and TIMP-1 in experimental groups when compared with their controls. Neither MMP-9 nor TIMP-2 antibodies stained the fibroblast cells kept in normoxic, hypoxic or hyperosmotic conditions.|
|Appears in Collections:||Scopus|
Web of Science
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.