Publication:
Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts

dc.contributor.buuauthorAkkoç, Ahmet
dc.contributor.buuauthorKahraman, Müjdat Müfit
dc.contributor.buuauthorCansev, Mehmet
dc.contributor.buuauthorÖzyiğit, Musa Özgür
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentPatoloji Ana Bilim Dalı
dc.contributor.departmentKlinik Farmakoloji Ana Bilim Dalı
dc.contributor.researcheridM-9071-2019
dc.contributor.researcheridAAH-2873-2021
dc.contributor.researcheridAAR-6478-2021
dc.contributor.scopusid55584229300
dc.contributor.scopusid6701778876
dc.contributor.scopusid8872816100
dc.contributor.scopusid6507338060
dc.date.accessioned2022-01-26T11:44:10Z
dc.date.available2022-01-26T11:44:10Z
dc.date.issued2011
dc.description.abstractThis study investigated the effects of hypoxic and hyperosmotic stresses on the matrix metalloproteinases 2 and 9 (MMP-2, MMP-9) and their tissue inhibitors 2 and 1 (TIMP-2, TIMP-1) respectively in cultured lung fibroblasts from broiler chickens. Isolated and sub-cultured primary lung fibroblasts from 1-day-old broilers were exposed to hypoxia and hyperosmosis. The presence of the enzymes and the effects of hypoxia and hyperosmosis on the enzymes in cultured lung fibroblasts, and in the culture media, were evaluated with the streptavidin-biotin-peroxidase method and western blotting using commercially available primary monoclonal antibodies. Short (6 hours) and long term (12 hours) hypoxia, hypoxia + hyperosmosis (6 hours) and hyperosmosis (6 hours) triggered the release of both MMP-2 and TIMP-1 in experimental groups when compared with their controls. Neither MMP-9 nor TIMP-2 antibodies stained the fibroblast cells kept in normoxic, hypoxic or hyperosmotic conditions.
dc.identifier.citationAkkoç, A. vd. (2011). ''Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts''. Avian Biology Research, 4(1), 6-16.
dc.identifier.endpage16
dc.identifier.issn1758-1559
dc.identifier.issn1758-1567
dc.identifier.issue1
dc.identifier.scopus2-s2.0-79954510188
dc.identifier.startpage6
dc.identifier.urihttps://doi.org/10.3184/175815511X13000117548916
dc.identifier.urihttps://eurekamag.com/research/064/056/064056732.php
dc.identifier.urihttp://hdl.handle.net/11452/24302
dc.identifier.volume4
dc.identifier.wos000296708500002
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherSage Publications
dc.relation.bap2000/47
dc.relation.journalAvian Biology Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgriculture
dc.subjectZoology
dc.subjectBroiler lung fibroblast
dc.subjectHypoxia
dc.subjectHyperosmosis
dc.subjectMatrix metalloproteinases
dc.subjectPulmonary-hypertension syndrome
dc.subjectRight-ventricular failure
dc.subjectEndothelial-cells
dc.subjectAscites syndrome
dc.subjectTissue inhibitors
dc.subjectDrinking-water
dc.subjectChickens
dc.subjectSodium
dc.subjectGrowth
dc.subjectHypertrophy
dc.subjectGallus gallus
dc.subject.emtreeGelatinase A
dc.subject.emtreeGelatinase B
dc.subject.emtreeTissue inhibitor of metalloproteinase 1
dc.subject.emtreeTissue inhibitor of metalloproteinase 2
dc.subject.emtreeAnimal cell
dc.subject.emtreeAnimal experiment
dc.subject.emtreeAnimal model
dc.subject.emtreeArticle
dc.subject.emtreeBroiler
dc.subject.emtreeCell culture
dc.subject.emtreeCell isolation
dc.subject.emtreeControlled study
dc.subject.emtreeEnzyme blood level
dc.subject.emtreeEnzyme release
dc.subject.emtreeHyperosmotic stress
dc.subject.emtreeHypoxia
dc.subject.emtreeLung fibroblast
dc.subject.emtreeMale
dc.subject.emtreeNonhuman
dc.subject.emtreeProtein expression
dc.subject.scopusRoss (Chicken Breed); Broiler Chickens; Ascites
dc.subject.wosAgriculture, dairy & animal science
dc.subject.wosOrnithology
dc.subject.wosZoology
dc.titleEffects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts
dc.typeArticle
dc.wos.quartileQ4
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Patoloji Ana Bilim Dalı
local.contributor.departmentTıp Fakültesi/Klinik Farmakoloji Ana Bilim Dalı
local.indexed.atScopus
local.indexed.atWOS

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