Publication: Immunohistochemical localization of transforming growth factor beta(1) and beta(2) in mouse testes during postnatal development
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Özgüden, Cansel Güzin Akkoç
Özer, Aytekin
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Taylor & Francis
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Abstract
We examined age-related changes in the expression of transforming growth factor-beta(1) (TGF-beta(1)) and transforming growth factor-beta(2) in mouse testes. The mice were assigned to three age groups: 35, 50, and 75 days old. Paraffin embedded testis sections were processed for the standard streptavidin biotin peroxidase complex immunohistochemistry method. TGF-beta(1) expression increased in aging round spermatids over the time studied. There was no expression in 35-day-old Leydig cells, whereas strong expression of TGF-beta(1) was observed in 50-day-old Leydig cells. Expression decreased in 75-day-old Leydig cells. TGF-beta(2) expression was weak in 35- and 50-day-old mouse spermatids, but expression was greater in 75-day-old elongated spermatids. In Leydig cells, TGF-beta(2) expression was strong in both 35- and 50-day-old mice, whereas the expression of TGF-beta(2) was less in 75-day-old Leydig cells. Our results suggest that TGF-beta(1) and TGF-beta(2) may play significant roles in testicular functions and germ cell development in mice.
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Keywords
Biotechnology & applied microbiology, Cell biology, Immunohistochemistry, Mouse, Testis, Tgf-beta(1), Tgf-beta(2), II receptors, Tgf-beta, Expression, Spermatogenesis, Protein, Mus
Citation
Özgüden C. G. A. ve Özer, A. (2012). "Immunohistochemical localization of transforming growth factor beta(1) and beta(2) in mouse testes during postnatal development". Biotechnic & Histochemistry, 87(2), 154-159.