Publication: Radioprotection by two phenolic compounds: Chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro
Date
Authors
Çinkılıç, Nilüfer
Çetintaş, Sibel Kahraman
Vatan, Özgür
Yılmaz, Dilek
Çavaş, Tolga
Tunç, Sema
Özkan, Lütfi
Bilaloǧlu, Rahmi
Authors
Zorlu, Tolga
Advisor
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Pergamon-Elsevier Science
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Abstract
The present study was designed to determine the radioprotective effect of two phytochemicals, namely, quinic acid and chlorogenic acid, against X-ray irradiation-induced genomic instability in non-tumorigenic human blood lymphocytes. The protective ability of two phenolic acids against radiation-induced DNA damage was assessed using the alkaline comet assay in human blood lymphocytes isolated from two healthy human donors. A Siemens Mevatron MD2 (Siemens AG, USA, 1994) linear accelerator was used for irradiation. The results of the alkaline comet assay revealed that quinic acid and chlorogenic acid decreased the DNA damage induced by X-ray irradiation and provided a significant radioprotective effect. Quinic acid decreased the presence of irradiation-induced DNA damage by 5.99-53.57% and chlorogenic acid by 4.49-48.15%, as determined by the alkaline comet assay. The results show that quinic acid and chlorogenic acid may act as radioprotective compounds. Future studies should focus on determining the mechanism by which these phenolic acids provide radioprotection.
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Keywords
Food science & technology, Toxicology, Radioprotection, Chlorogenic acid, Quinic acid, X-ray irradiation, Comet assay, DNA damage, Cultured human-lymphocytes, Gamma-radiation, Caffeic acid, Lipid-peroxidation, Antioxidant activities, Derivatives, Cells, Carcinogenesis, Flavonoids, Mechanism
Citation
Çinkılıç, N. vd. (2013). "Radioprotection by two phenolic compounds: Chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro". Food and Chemical Toxicology, 53, 359-363.