2005 Cilt 24 Sayı 1-2-3-4

Permanent URI for this collectionhttps://hdl.handle.net/11452/13143

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Alterations in some blood parameters after high level ethanol ıntake
(Uludağ Üniversitesi, 2005-06-07) Yağcı, Artay; Yalçın, Murat; Uludağ Üniversitesi/Veteriner Fakültesi.
The effects of high level ethanol intake on some blood parameters over a 50 days period were examined in 36 Sprague Dawley rats divided into 3 treatment groups: Ad libitum fed control group (n=12), sucrose group (n=12) fed with solution of sucrose so calorie intake equated with the ethanol group, and ethanol group (n=12) added ethanol (15 % v/v) in drinking water. Significant decreases (p<0.05) were noted in ethanol group’s white- and red-blood cell counts, haemoglobin concentration, erythrocyte diameter, erythrocyte sodium and potassium levels, while significant (p<0.05) increases were observed in their mean corpuscular volume and mean corpuscular haemoglobin levels. No changes were observed in hematocrit level. Results of this study suggest that high level ethanol intake in rats causes alterations in blood cells count and in erythrocyte diameter and in erythrocyte element composition and this negative effect of ethanol on blood parameters did not stem from the energy of ethanol.
Assessment of microbiological contamination factors in frozen stuffed snail processing stages
(Uludağ Üniversitesi, 2006-02-28) Günşen, Uğur; Dokuzlu, Uğur; Temelli, Seran; Şen, M. K. Cem; Uludağ Üniversitesi/Veteriner Fakültesi.
In this study, six predetermined processing stages and six possible contamination sources in frozen stuffed snail production were examined for the enumeration of total aerobic mesophilic bacteria, coliforms, Escherichia coli (E. coli), coagulase positive staphylococci and for the detection of Listeria spp. and Salmonella spp. Results revealed that the cooling stage was a major contamination factor. Also, shells, although ‘pasteurized’, seemed to be one of the causes for the increases in the final microbial counts of the product. Another minor source for recontamination was determined as the cooling vat. Improvements were recommended for more effective technology applications, which would eliminate risks for contamination for the product.
Determination of oxytetracycline, penicillin G and sulphadimidine residues in cow milks in Bursa
(Uludağ Üniversitesi, 2005-05-04) Oruç, H. Hüseyin; Sonal, Songül; Uludağ Üniversitesi/Veteriner Fakültesi.
Oxytetracycline, penicillin G (benzylpenicillin) and sulphadimidine levels were analysed by highperformance liquid chromatography (HPLC) methods in 25 raw cow milk samples, which were collected from plain and mountain villages during March-April 2003 in Bursa province. None of the antibacterials were detected in the milk samples suggesting that the milk samples are not a risk for consumer health.
Determination of seroprevalence of listeria monocytogenes antibodies in cattle in bursa province of Turkey
(Uludağ Üniversitesi, 2006-03-07) Babur, Cahit; Kılıç, Selçuk; Kennerman, Engin; Uludağ Üniversitesi/Veteriner Fakültesi.
To determine the seroprevalance of antibodies to L. monocytogenes in cattle in Bursa province of Turkey was aimed in this study. Two hundred nine, aged 1-5 years, healthy Holstein cattle were randomly selected from 6 different districts and after clinical examination blood samples were collected. The herd size (number of cattle in each herd) and the animals fed silage were recorded. Antibodies to L. monocytogenes were determined by agglutination test according to the method described by of Osebold et al. Hematological parameters (white blood cell count, circulating red blood cell count, hematocrit and differenciated cell counts) were analyzed. Antibodies to L. monocytogenes were found in 101 (48.3 %) of the 209 cattle tested. Of 209 cattle tested 54 (25.8%) had 1/50, 74 (35.4%) had 1/100, 23 (11.0%) had 1/200 and 4 had (1.9%) 1/400 agglutination titer whereas no agglutination was detected in 54 (24.88%) cattle. 53.7% (58/108) of female and 42.5% (43/101) of male cattle have been found to be seropositive. The seropositivity in silage fed cattle (57.5%, 92/160) was found statistically higher than cattle that were not feed silage (18.3%, 9/49) (p<0.001). The higher seropositivity (55.8%) was observed in cattle from large herd size (50 – 100 cattle). As a result, in this study seroprevalence of antibodies to Listeria monocytogenes in cattle in Bursa province of Turkey was found as 48.3 % and it was concluded that silage feeding is an important factor in the epidemiology of listeriosis. Larger and detailed prophylactic studies must be planned to control this zoonosis infection for animal and human health
Effects of feeding with molasses and vibrotal on some serum biochemical parameters and liveweight gains in lambs
(Uludağ Üniversitesi, 2005-03-25) Polat, Ümit; Yalçın, Abdullah; Çetin, Meltem; Ak, İbrahim; Doğrutekin, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Ziraat Fakültesi.
This study was performed to investigate the effects of feeding with molasses and vibrotal on biochemical blood parameters and live weight gain in lambs. For this purpose, forthy male weanling Merino lambs were used. The lambs were divided into four groups. Each group was fed with a different experimental diet containing various level of molasses or vibrotal (3% molasses, 6% molasses, 3% vibrotal and 6% vibrotal) for 56 days. Blood samples were collected on the days of 0 and 56th of the experimental period and biochemical analyses were performed. Body weight of each lamb was recorded at biweekly intervals. Among the groups, only urea values exhibited statistical differences (p<0.05). Glucose, cholesterol, total protein, urea levels and ALT, AST activities were found significantly different at various levels on the days of 0 and 56th of the experimental period in each group. While the liveweight gains were not different on the weighing days among groups, total liveweight gains had a statistical significance (p<0.05) between 3% molasses and 6% vibrotal groups. The results of the present study showed that feeding with molasses and vibrotal at various levels had no significant effect on the metabolisms of lambs and that vibrotal which is cheaper than molasses can be used instead of molasses. However, usage of vibrotal should be limited in the rate of 3 %, since the addition of 6 % vibrotal to diet decreases total liveweight gain and increases the serum urea levels.
Heat treated Turkish style sucuk: Evaluation of microbial contaminations in processing steps
(Uludağ Üniversitesi, 2006-02-28) Temelli, Seran; Anar, Sahsene; Sen, M. K. Cem; Beyaz, Devrim; Uludağ Üniversitesi/Veteriner Fakültesi.
In this work, we determined the trends in contaminations for important microbial groups in heat treated Turkish style sucuk processing and verified the contamination routes for these groups. Samples were taken from deboned and cubed meat, post-blending and kneading, post-grinding, post-filling and from the final product after heat treatment. We also analysed samples of spices, casing, knife, meat cutting surface, batter vat, grinding machine, filling machine, workers’aprons, workers’ hands, potable water used in the plant and production and cold room area air as possible origins for contamination and/or recontamination. Statistical data revealed the following enlisted sources as primary agent(s) of contamination at indicated steps: knife (enterococci, p<0.001), meat cutting surface and cold room area (total aerobic mesophilic bacteria, p<0.05) in deboning; spices (enterococci, yeast and mold, p<0.01) in post-blending; batter vat (yeast and mold, p<0.05) and grinding machine (enterococci, p<0.001) in post-grinding; filling machine (total aerobic mesophilic bacteria, p<0.05; coliform, p<0.01) and casing (coliform, p<0.01; yeast and mold, p<0.001) in post-filling. Workers’ hands were very important contamination/recontamination sources in blending (enterococci, staphylococci, yeast and mold, p<0.05), in grinding (enterococci, p<0.001; staphylococci, p<0.05) and in filling (coliform, p<0.001) steps.
Influence of acute serious haemorrhagic shock on erythrocyte glutathione and sodium – potassium contents
(Uludağ Üniversitesi, 2005-03-27) Yalçın, Murat; Galip, Nurten; Uludağ Üniversitesi/Veteriner Fakültesi.
The aim of the present study is to describe erythrocyte glutathione level and sodium – potassium contents at acute serious haemorrhage. The experiment was performed on adult male 15 Sprague-Dawley rats. Haemorrhage was performed by withdrawing a total volume of 2.1 ml of blood / 100 g body weight over a period of 10 min. Blood was collected twice; before haemorrhage and 1 hour after haemorrhage. Haemorrhage caused approximately 20 % decrease in hematocrit, red blood cells count, amount of haemoglobin and about 60 % decrease in mean arterial pressure but 46 % increase in heart rate. Interestingly, erythrocyte glutathione value does not change in pre- and post-haemorrhage. Moreover, haemorrhage increased plasma and erythrocyte sodium values but decreased plasma and erythrocyte potassium values. In conclusion, due to hemorrhagic shock, increased production of reactive oxygen species is a feature of haemorrhagic shock and decreased erythrocytes by haemorrhage can respond to oxidative stress by upregulating antioxidant defence in terms of increased production of glutathione. Moreover, the observed high sodium concentrations associated with a potassium decrease in red blood cell can be defect in cellular function due to severe hemorrhagic shock.
New chalicotheriidae (perissodactyla-mammalia) fossil from the middle miocene of turkey (Bursa-Orhaneli)
(Uludağ Üniversitesi, 2006-01-23) Yıldız, Bahri; Onar, Vedat; Uludağ Üniversitesi/Veteriner Fakültesi.
The Chalicotheriidae fossils described in this paper were found by a certain Mustafa Özyıldız during clay subsoil cleaning at the coal-mine Bursa-Orhaneli (Burmu) in Western Anatolia. The duplex bone (joint middle and proximal phalanx) belongs most probably to the second digit of the manus. The epiphyseal parts of vertebrae found among the fossils point to a young animal of the assumed Ancylotherium species. All bones – with the exception of duplex and metacarpal II – are too fragmented. However, the authors are strongly inclined to believe that they are either of Metaschizotherium or Ancylotherium origin. In particular duplex and metacarpal II are considered the most characteristic bones for such an attribution. The teeth among the fossil material are those of Chalicotheriidae which contributed to the conclusion that the remains are to be classified as those of Metaschizotherium species.

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