Journal of Biological and Environmental Sciences

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Browsing by Department "Gıda Hijyeni ve Teknolojisi Bölümü"

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    A microbiological evaluation on the ready-to-eat red meat and chicken donair kebabs from a local catering company in Bursa
    (Uludağ Üniversitesi, 2009) Yuksek, Nur; Evrensel, Surreya Saltan; Temelli, Seran; Anar, Sahsene; Sen, M. K. Cem; Teknik Bilimler Meslek Yüksekokulu; Gıda Hijyeni ve Teknolojisi Bölümü
    This study was performed to evaluate the microbiological quality of the ready-to-eat red meat and chicken donairs from a catering company in Bursa. Samples were examined for total aerobic mesophilic bacteria, coliforms, Escherichia coli (E. coli), enterococci, staphylococci, coagulase positive staphylococci, and Salmonella spp. Temperatures used in this company were found sufficient for donair surface cooking. The collection tray and the personnel hands were determined as the main cross and/or post contamination sources for the donair ready for service.
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    A PCR- ELISA for the detection of Salmonella from chicken intestine
    (Uludağ Üniversitesi, 2007-04-01) Eyigör, Ayşegül; Goncagül, Gülşen; Çarlı, K. Tayfun; Veteriner Fakültesi; Gıda Hijyeni ve Teknolojisi Bölümü
    In this study, a Polymerase Chain Reaction Enzyme Linked Immunosorbent Assay (PCR-ELISA) was described to detect Salmonella DNA from selective primary enrichment culture of chicken intestine. Salmonella genus-specific PCR product was produced using invA-specific primers and digoxigenin by commercial PCR ELISA DIG Labelling and ELISA DIG Detection kits. PCR-ELISA detection limits with S. Enteritidis 64K DNA, pure culture, and with the intestinal homogenate artificiallycontaminated with this strain were found as 0.0079 µg ml-1, 50 cfu ml-1, and 70 cfu ml-1, respectively. Sensitivity and specificity of the assay was determined as 100% with the tests performed with 41 Salmonella enterica serovar DNAs, with non-Salmonella strains, Citrobacter sp., E. coli, Klebsiella sp., P. aeruginosa, and Streptococcus sp. DNAs, and with 124 tetrathionate broth enrichment cultures of Salmonella-contaminated chicken intestinal samples. We suggest that this PCR-ELISA detection could be an alternative method to detect Salmonella-specific DNA from chicken intestine, and it would find use particularly in highsample throughput laboratories in the poultry sector.