PubMed

Permanent URI for this collectionhttps://hdl.handle.net/11452/24864

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Effect of brining on biological activity of leaves of Vitis vinifera L. (Cv. Sultani Cekirdeksiz) from Turkey
(American Chemical Society, 2007-05-30) Koşar, Müberra; Küpeli, Esra; Başer, K. Hüsnü Can; Malyer, Hulusi; Uylaser, Vildan; Türkben, Cihat; Fen Edebiyat Fakültesi; Biyoloji Bölümü; AAG-8410-2021; 6602736554; 8228159700; 6506365712
Leaves of Vitis vinifera (Fam. Vitaceae) cv. 'Sultani Çekirdeksiz' cultivated in Manisa-Alaşehir in western Turkey, were processed with or without brine. Fresh, brined, and nonbrined leaves (after being subjected to 3 months of fermentation) were sampled and extracted with distilled water under reflux. Antioxidant, anti-inflammatory, and anti-nociceptive activities of the water extracts were investigated using in vitro and in vivo methods. Free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH • assay), iron(III) reductive activity (reducing power activity assay), capacity of inhibition of linoleic acid peroxidation (ferric thiocyanate and thiobarbituric acid method), anti-nociceptive activity (p-benzoquinone- induced abdominal constriction test), and anti-inflammatory activity (carrageenan-induced hind paw edema model) were used to determine biological activities of the extracts. In addition, the contents of total phenolics, flavonoids, and flavonols in the extracts were determined by spectrophotometrical methods. Results were compared with those of ascorbic acid, butylated hydroxytoluene, and gallic acid as reference antioxidants. The extracts of fresh, brined, and nonbrined leaves showed almost the same activity in all antioxidant assays. These extracts inhibited the oxidation of linoleic acid to the same extent as BHT. Compositions of the extracts were analyzed by a reverse phase HPLC-PDA method. The occurrence of hydroxycinnamic acids (e.g., caffeic acid) and flavonoids (e.g., quercetin) was verified in the extracts. The content of total flavonoids as well as quercetin was increased by fermentation.
Genetic variation and relationships between Azerbaijani and Turkish olive genetic resources
(Springer, 2022-06) Duran, Sevin Teoman; Aghayeva, Saltanat; Akparov, Zeynal; Mammadov, Ayaz; Asgarova, Rana; Uslu, Osman Yaşar; Kırıkoğlu, Osman; Duran, Ufuk Tan; İpek, Meryem; Barut, Erdogan; Ercişli, Sezai; İlhan, Gülce; İpek, Ahmet; TEOMAN DURAN, SEVİN; İPEK, MERYEM; İPEK, AHMET; BARUT, ERDOĞAN; Kırıkoğlu, Osman; Duran, Ufuk Tan; Karacabey Meslek Yüksekokulu; Süt ve Besi Hayvancılığı Bölümü; Organik Tarım Programı; 0000-0003-1469-6777; 0000-0001-5821-2426; AAE-4675-2019; AAH-3233-2021; AAK-4655-2021; AAE-6913-2019; FCC-3703-2022; IDH-4027-2023
Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalik 1'and 'Ayvalik 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalik" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.
Genotype-dependent gene expression in strawberry (Fragaria x ananassa) plants under high temperature stress
(Springer/Plenum Publishers, 2020-06-04) Ergin, Sergül; Gülen, Hatice; İpek, Ahmet; Ersoy, Figen; Kesici, Müge; Ziraat Fakültesi; Moleküler Biyoloji ve Genetik Bölümü; 0000-0002-9136-3186; 0000-0003-2267-069X; AAI-6817-2021; ABD-6710-2020; 54412311700; 6603912487; 55088196700
The differences in tolerance to high temperatures were investigated on the basis of gene expressions in two strawberry (Fragaria x ananassaDuch) cultivars which were previously determined as high temperature tolerant (Redlands Hope = R. Hope) and sensitive (Festival). Plants were exposed incrementally to 35, 40, 45, and finally 50 degrees C for 24 h. qRT-PCR analyses were carried out with 19 known sequences from the databases. Protein expression analyses were based on SDS-PAGE results, sequenced and then separated due to their isoelectric points. Expression levels were determined at 35, 40, and 45 degrees C. According to the results, tolerance of 'R. Hope' to high temperature stress can be explained with the coordination of Hsp70, Hsp90, and small heat shock proteins (sHsps) having a vital and supplementary role in stress response. Sensitive cultivar 'Festival' can respond to high temperatures only with the low molecular weight protein and transcripts that do not take a central role in high temperature stress response. Moreover, allergen gene expression triggered by high temperature were detected in both cultivars with different expression levels. The greater expression level in allergen genes observed in the sensitive cultivar 'Festival' under high temperature indicates that there is possibly a negative correlation between expression level in allergen genes and heat stress tolerance. Future studies addressing allergen gene expression under high temperature stress are required to confirm on these findings and to expand on the potential use as a molecular marker in breeding process for enhanced tolerance to high temperature.
Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits
(Fun-pec Editora, 2015-03-31) Şeker, Murat; Gül, M. K.; İpek, Meryem; İpek, Ahmet; Ziraat Fakültesi; Bahçe Bitkileri Bölümü; 0000-0002-0609-3442; 0000-0002-9136-3186; AAH-3233-2021; 16031208900; 6603912487
The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with a-tocopherol and.-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization.
Soluble sugars and sucrose-metabolizing enzymes related to cold acclimation of sweet cherry cultivars grafted on different rootstocks
(Hindawi Publishing Corporation, 2012) Turhan, Ece; Ergin, Sergül; Ziraat Fakültesi; Bahçe Bitkileri Bölümü; 0000-0002-7720-5536; AAG-6558-2020; 39661052000
The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA) and nonacclimated (NA) stages. Bark tissues subjected to 4 degrees C and -5 degrees C injured to a limited extent in both stages. However, more than 50% injury occurred by temperatures equal to or colder than -15 degrees C only in NA period. Total soluble sugar (TSS), reducing sugars, and sucrose contents were higher in CA than those in NA stages in all samples. The activities of acid invertase (EC 3.2.1.26) and sucrose synthase (SS) (EC 2.4.2.13) enzymes were higher in NA stage than those in CA stage. Considering the rootstocks, reducing sugars were higher in both cultivars grafted on Gisela 5 whereas sucrose contents were higher in both cultivars grafted on Mazzard. However, the enzyme activities of both cultivars were higher on Mazzard rootstock than on Gisela 5. In conclusion, cold hardiness of sweet cherry graft combinations was suggested by increasing their TSS, reducing sugars, and sucrose contents significantly in the CA stage. Moreover, acid invertase and SS are down regulated during cold acclimation. Indeed the results suggested that Mazzard is more cold-hardy rootstock than Gisela 5.

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