Endogenous promoters and ınhibitors in olive cuttings in relation to adventitious root formation

Abstract

Levels of endogenous rooting promoters and inhibitors were determined in olive cvs "Roghani"(high rooting ability), "Tokhmkabki" and "Konservalia"(low rooting abilities). Cuttings were prepared and rooted in greenhouse under intermittent mist after that, samples for each cultivar were taken 60 days after cuttings preparation. Thin layer chromatography was used for purification and identification of endogenous plant hormones (Indol-3-acetic acid (IAA), Abscisic acid (ABA), Gibberellins (GAs) and cytokinins). Results indicated that "Roghani" olive cuttings gave significantly higher rooting percentage than "Tokhmkabki" and "Konservalia". Results showed significant differences in IAA concentration in leaves of "Roghani", "Tokhmkabki" and "Konservalia" at sampling date. IAA concentration in "Roghani" leaves was significantly higher than "Tokhmkabki" and "Konservalia" cultivars. These results suggest that auxin was confirmed as limiting factor of root initiation. However, insignificant differences in ABA and cytokinins concentration were recorded between the 3 olive cultivars. But the high GAs concentration in "Tokhmkabki" and "Konservalia" leaves coincides with the low rooting percentages of the cuttings.