Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience

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dc.contributor.buuauthorToker, M. Berk
dc.contributor.buuauthorAlçay, Selim
dc.contributor.buuauthorGökçe, Elif
dc.contributor.buuauthorÜstüner, Burcu
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-7678-3289tr_TR
dc.contributor.orcid0000-0003-4033-9749tr_TR
dc.contributor.researcheridAAG-7238-2021tr_TR
dc.contributor.researcheridABA-6294-2020tr_TR
dc.contributor.scopusid56480349200tr_TR
dc.contributor.scopusid56099810300tr_TR
dc.contributor.scopusid56779799700tr_TR
dc.contributor.scopusid18937724600tr_TR
dc.date.accessioned2022-10-11T06:17:38Z
dc.date.available2022-10-11T06:17:38Z
dc.date.issued2016-06
dc.description.abstractThe scope of this study was investigation the affects of various antioxidants on 1% soybean lecithin-based semen extenders for ram semen cryopreservation. Ejaculates, collected via electrically stimulated ejaculation, that have a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. The pooled samples were split into four equal aliquots as 5 mM Methionine, 5 mM Cysteamine, 1 mM Cysteine and a sample of antioxidant-free control group. Each sample group was diluted to a ratio of 1/5 (semen/extender, v/v) as final concentration and two step dilution method was used for cryopreservation. Extender groups were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Semen samples also incubated for 6 h in humidified air with 5% CO2 at 39 degrees C to evaluate post-thaw incubation resilience of semen characteristics. The results showed that freezing and thawing procedures had negative effects on motility (P < 0.05), plasma membrane integrity (P < 0.05) and acrosomal integrity (P < 0.05). After 6 h of incubation time, the Cysteine supplemented extender group yielded significantly higher results than other extender groups in terms of spermatological parameters. Furthermore MDA levels in the antioxidant groups were lower than control group (P < 0.05). Nevertheless, there were no significant differences among antioxidant groups.en_US
dc.identifier.citationToker, M. B. vd. (2016). "Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience". Cryobiology, 72(3), 205-209.en_US
dc.identifier.endpage209tr_TR
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue3tr_TR
dc.identifier.pubmed27157891tr_TR
dc.identifier.scopus2-s2.0-84969980358tr_TR
dc.identifier.startpage205tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2016.05.001
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0011224016300438
dc.identifier.urihttp://hdl.handle.net/11452/29040
dc.identifier.volume72tr_TR
dc.identifier.wos000377820500004tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.bapKUAP(V)-2015/55en_US
dc.relation.journalCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLife sciences & biomedicine - other topicsen_US
dc.subjectPhysiologyen_US
dc.subjectSoybean lecithinen_US
dc.subjectIncubation resilienceen_US
dc.subjectAntioxidanten_US
dc.subjectRam semenen_US
dc.subjectCryopreservationen_US
dc.subjectYolk-based extenderen_US
dc.subjectEgg-yolken_US
dc.subjectGoat semenen_US
dc.subjectIn-vitroen_US
dc.subjectOxidative parametersen_US
dc.subjectPostthawing qualityen_US
dc.subjectField-fertilityen_US
dc.subjectDNA integrityen_US
dc.subjectSpermatozoaen_US
dc.subjectSpermen_US
dc.subject.emtreeAgglutininen_US
dc.subject.emtreeCysteineen_US
dc.subject.emtreeDNA nucleotidylexotransferaseen_US
dc.subject.emtreeFluorescein isothiocyanateen_US
dc.subject.emtreeMalonaldehydeen_US
dc.subject.emtreeMercaptamineen_US
dc.subject.emtreeMethionineen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreeAntioxidanten_US
dc.subject.emtreeCryoprotective agenten_US
dc.subject.emtreeCysteineen_US
dc.subject.emtreeMercaptamineen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreeAcrosomeen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell membraneen_US
dc.subject.emtreeCellular parametersen_US
dc.subject.emtreeConcentration (parameters)en_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeIncubation timeen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeRam (sheep)en_US
dc.subject.emtreeSemen analysisen_US
dc.subject.emtreeSoybeanen_US
dc.subject.emtreeSpermen_US
dc.subject.emtreeSperm qualityen_US
dc.subject.emtreeSpermatozoon motilityen_US
dc.subject.emtreeSupplementationen_US
dc.subject.emtreeTUNEL assayen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.emtreeFreezingen_US
dc.subject.emtreeProceduresen_US
dc.subject.emtreeSheepen_US
dc.subject.emtreeSoybeanen_US
dc.subject.emtreeSperm preservationen_US
dc.subject.meshAcrosomeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntioxidantsen_US
dc.subject.meshCryopreservationen_US
dc.subject.meshCryoprotective agentsen_US
dc.subject.meshCysteamineen_US
dc.subject.meshCysteineen_US
dc.subject.meshFreezingen_US
dc.subject.meshIn situ nick-end labelingen_US
dc.subject.meshLecithinsen_US
dc.subject.meshMaleen_US
dc.subject.meshSemenen_US
dc.subject.meshSemen preservationen_US
dc.subject.meshSheepen_US
dc.subject.meshSoybeansen_US
dc.subject.meshSperm motilityen_US
dc.subject.scopusExtender; Semen; Semen Extendersen_US
dc.subject.wosBiologyen_US
dc.subject.wosPhysiologyen_US
dc.titleCryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilienceen_US
dc.typeArticle
dc.wos.quartileQ2 (Biology)en_US
dc.wos.quartileQ3 (Physiology)en_US

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