Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography

Abstract

Gold-of-pleasure or false flax (Camelina sativa L Crantz) is being developed as an alternative oil crop for biodiesel and for food use. The seed meal, which contains three relatively unique glucosinolates, is being evaluated for approval for use as an ingredient in animal feeds and for other uses. The objective of this research was to develop reproducible methods for the isolation of large quantities of pure camelina glucosinolates (glucoarabin, glucocamelinin, and 11-(methylsulfinyl)-undecylglucosinolate) and develop efficient methods for quantifying these compounds. The separation and purification of the camelina glucosinolates were achieved using a combination of reverse phase chromatography, counter-current chromatography and ion exchange chromatography. An efficient reverse phase HPLC separation method was used to quantitate the glucosinolate content in camelina seed and plant extracts. The quanti ration methodology was used to measure glucosinolate levels in seeds from 30 cultivars grown in a U.S. field trial and measure glucosinolates levels in sprouted camelina seeds.