Ratlarda kardiyak mastositlerinin istatistiksel olarak dağılımı ve heterejonitesi
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Date
2015-05-12
Authors
Erekli, Özlem
Çınar, Kenan
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Bu çalışmada Wister Albino türü sıçanlarda kardiyak mast hücrelerinin sayısal dağılımı ve altı farklı tespit çözeltisinin (Bouin, %10 Formalin, Schaudin, Orth, Alkolik Formalin ve Carnoy) bu hücrelerin boyanma özelliklerine olan etkisinin belirlenmesi amaçlandı. Mast hücrelerinin demonstrasyonu için Toluidin Blue (TB), Thionin ve Alcian Blue/Safranin O (AB/SO) boya metotları uygulandı. Boyanan kesitlerde, tüm preparatlardaki mast hücrelerinin sayısal yoğunluğu oküler mikrometre yardımıyla belirlendi. Daha sonra elde edilen veriler 1mm2 ’lik alandaki mast hücre sayısına dönüştürüldü. TB/ Thionin/ AB/SO ile boyanan mast hücre sayılarının atriyum ve ventrikuluslardaki yoğunlukları tespit edildi. Mast hücreleri Carnoy tespit çözeltisi ile Toluidine Blue'da, Orth tespit çözeltisiyle ise Thionin ve AB/SO'de en fazla sayısal sonucu vermiştir. Altı fiksatifin de uygulanması sonucunda mast hücre granülleri en belirgin olarak AB/SO boyanması ile gözlendi. Mast hücre şekillerinin, farklı tespit çözeltilerine göre farklılık gösterdiği saptandı. %10 Formalin tespit çözeltisi hariç diğer tespit çözeltileri ile fiske edilmiş doku örnekleri, Toluidine Blue boyası ile boyandıklarında mast hücrelerinin metakromazi gösterdikleri tespit edildi. Carnoy ve Schaudin tespit çözeltilerinin kullanıldığı doku örneklerinde, Thionin ile boyandıklarında metakromazi gösteren mast hücreleri görüldü
This study was aimed to determine the numerical distribution of cardiac mast cells and the effect of six different fixatives (Bouin, 10% Formalin, Schaudin, Orth, Alcoholic Formalin and Carnoy) on the staining properties of these cells in Albino Wistar rats. Toluidine Blue (TB), Thionin and Alcian Blue / Safranin O (AB/SO) staining procedures were employed to demonstrate the cardiac mast cells. Numerical density of mast cells was determined through the instrument of the ocular micrometer. Then, the data obtained were converted to the number of mast cells in a 1mm2 area. Numerical density of TB/Thionin/AB/SO-stained mast cells was detected in atrium and ventricles. Mast cells were detected to outnumber in Carnoy-fixed and TB-stained sections and in Orth-fixed and AB/SO, Thionin stained sections. Mast cell granules were demonstrated more specifically in AB/SO staining as a result of the implementation of six different fixatives. Shapes of mast cells were found to vary by different fixative solutions. Mast cells were observed to stain metachromatically when sections fixed with other fixative solutions except for 10% Formalin fixative solution were stained with TB. In a similar way, mast cells were shown to stain metachromatically when sections fixed with Carnoy and Schaudin fixative solutions were stained with Thionin.
This study was aimed to determine the numerical distribution of cardiac mast cells and the effect of six different fixatives (Bouin, 10% Formalin, Schaudin, Orth, Alcoholic Formalin and Carnoy) on the staining properties of these cells in Albino Wistar rats. Toluidine Blue (TB), Thionin and Alcian Blue / Safranin O (AB/SO) staining procedures were employed to demonstrate the cardiac mast cells. Numerical density of mast cells was determined through the instrument of the ocular micrometer. Then, the data obtained were converted to the number of mast cells in a 1mm2 area. Numerical density of TB/Thionin/AB/SO-stained mast cells was detected in atrium and ventricles. Mast cells were detected to outnumber in Carnoy-fixed and TB-stained sections and in Orth-fixed and AB/SO, Thionin stained sections. Mast cell granules were demonstrated more specifically in AB/SO staining as a result of the implementation of six different fixatives. Shapes of mast cells were found to vary by different fixative solutions. Mast cells were observed to stain metachromatically when sections fixed with other fixative solutions except for 10% Formalin fixative solution were stained with TB. In a similar way, mast cells were shown to stain metachromatically when sections fixed with Carnoy and Schaudin fixative solutions were stained with Thionin.
Description
Keywords
Rat, Mast cell, Mast hücresi, Heart, Heterogeneity, Fixation, Kalp, Heterojenite, Fikzasyon
Citation
Erekli, Ö. ve Çınar, K. vd. (2015). "Ratlarda kardiyak mastositlerinin istatistiksel olarak dağılımı ve heterejonitesi". Uludağ Üniversitesi Veteriner Fakültesi, 34(1-2), 25-33.