Histologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosis

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dc.contributor.buuauthorAcar, Hilal
dc.contributor.buuauthorÇerçi, Ece
dc.contributor.buuauthorKhezri, Marzieh Karimi
dc.contributor.buuauthorÇetin, Melike
dc.contributor.buuauthorCanatan, Uygur
dc.contributor.buuauthorŞen, Mehmet Metin
dc.contributor.buuauthorAslan, Vildan
dc.contributor.buuauthorSarıl, Canan Altıncı
dc.contributor.buuauthorTemiz, Elif Mekik
dc.contributor.buuauthorTaşkapılıoğlu, Mevlüt Özgür
dc.contributor.buuauthorErdost, Hatice
dc.contributor.buuauthorSalcı, Hakan
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.tr_TR
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0001-5472-9065tr_TR
dc.contributor.orcid0000-0003-0154-9938tr_TR
dc.contributor.orcid0000-0001-9650-0891tr_TR
dc.contributor.orcid0000-0001-5323-6891tr_TR
dc.contributor.orcid000-0003-1547-7293tr_TR
dc.contributor.orcid0000-0001-6548-8754tr_TR
dc.contributor.researcheridAAH-9216-2021tr_TR
dc.contributor.researcheridAAI-6861-2021tr_TR
dc.contributor.researcheridAAW-5254-2020tr_TR
dc.contributor.researcheridABB-8161-2020tr_TR
dc.contributor.scopusid57209653860tr_TR
dc.contributor.scopusid57212212053tr_TR
dc.contributor.scopusid57216947212tr_TR
dc.contributor.scopusid56393942900tr_TR
dc.contributor.scopusid57189443508tr_TR
dc.contributor.scopusid57216950078tr_TR
dc.contributor.scopusid57200994296tr_TR
dc.contributor.scopusid57216954758tr_TR
dc.contributor.scopusid57216945905tr_TR
dc.contributor.scopusid25936798300tr_TR
dc.contributor.scopusid6505787570tr_TR
dc.contributor.scopusid8680329000tr_TR
dc.date.accessioned2023-01-26T05:52:31Z
dc.date.available2023-01-26T05:52:31Z
dc.date.issued2020-03-23
dc.description.abstractIn this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed. Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7). Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method. The left sciatic nerve of all rats was evaluated for EMG as control. Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture. EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8th weeks. EMG results between and within the groups were statistically evaluated by one-way analysis of variance. Rats in all groups were sacrificed by decapitation at PO 8th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues. EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8th week. Amplitude and conduction velocity increased gradually in all groups. In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8th week. Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8th week in all groups (P>0.05). In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively. In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV. As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.en_US
dc.identifier.citationAcar, H. vd. (2020). "Histologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosis". Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4), 483-490.tr_TR
dc.identifier.endpage490tr_TR
dc.identifier.issn1300-6045
dc.identifier.issue4tr_TR
dc.identifier.scopus2-s2.0-85085477164tr_TR
dc.identifier.startpage483tr_TR
dc.identifier.urihttps://doi.org/10.9775/kvfd.2019.23576
dc.identifier.urihttps://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2697.pdf
dc.identifier.urihttp://hdl.handle.net/11452/30652
dc.identifier.volume26tr_TR
dc.identifier.wos000537457100005tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.trdizinTrDizintr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherKafkas Üniversitesitr_TR
dc.relation.bapBUAP(V)-2015/3tr_TR
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectElectromyographyen_US
dc.subjectHistologyen_US
dc.subjectNeuroanastomosisen_US
dc.subjectRaten_US
dc.subjectStromal vascular fractionen_US
dc.subjectPeripheral-nerve regenerationen_US
dc.subjectFibrin glueen_US
dc.subjectHyaluronic-aciden_US
dc.subjectStem-cellsen_US
dc.subjectRepairen_US
dc.subjectRaten_US
dc.subjectTissueen_US
dc.subjectSutureen_US
dc.subjectDefecten_US
dc.subjectVeterinary sciencesen_US
dc.subject.emtreeEnrofloxacinen_US
dc.subject.emtreeFibrin glueen_US
dc.subject.emtreeKetamineen_US
dc.subject.emtreeMeloxicamen_US
dc.subject.emtreeXylazineen_US
dc.subject.emtreeAdipose tissueen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeAmplitudeen_US
dc.subject.emtreeAnimal experimenten_US
dc.subject.emtreeAnimal tissueen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell therapyen_US
dc.subject.emtreeConnective tissueen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDecapitationen_US
dc.subject.emtreeElectromyographyen_US
dc.subject.emtreeElectrophysiologyen_US
dc.subject.emtreeEpineural repair techniqueen_US
dc.subject.emtreeGastrocnemius muscleen_US
dc.subject.emtreeHstologyen_US
dc.subject.emtreeInflammatory cellen_US
dc.subject.emtreeLatent perioden_US
dc.subject.emtreeLower leg muscleen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMembrane stabilizationen_US
dc.subject.emtreeMicrosurgeryen_US
dc.subject.emtreeMotor nerve conductionen_US
dc.subject.emtreeMuscle action potentialen_US
dc.subject.emtreeMuscle contractionen_US
dc.subject.emtreeNerve anastomosisen_US
dc.subject.emtreeNerve cell stimulationen_US
dc.subject.emtreeNerve conductionen_US
dc.subject.emtreeNerve conduction velocityen_US
dc.subject.emtreeNerve regenerationen_US
dc.subject.emtreeNervous tissueen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeRaten_US
dc.subject.emtreeSciatic nerveen_US
dc.subject.emtreeStromal vascular fractionen_US
dc.subject.emtreeTibialis cranialis muscleen_US
dc.subject.emtreeTissue reactionen_US
dc.subject.scopusNerve Regeneration; Scaffold; Peripheral Nerve Injuriesen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleHistologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosisen_US
dc.title.alternativeNöroanastomoz sonrası stromal vasküler fraksiyonun nörorejeneratif etkisinin histolojik ve elektromiyografik değerlendirilmesitr_TR
dc.typeArticle
dc.wos.quartileQ4en_US

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