Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage
dc.contributor.author | Ulukaya, Engin | |
dc.contributor.buuauthor | Aybastıer, Önder | |
dc.contributor.buuauthor | Dawbaa, Sam | |
dc.contributor.buuauthor | Demir, Cevdet | |
dc.contributor.buuauthor | Akgün, Oğuzhan | |
dc.contributor.buuauthor | Arı, Ferda | |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü. | tr_TR |
dc.contributor.orcid | 0000-0002-0380-1992 | tr_TR |
dc.contributor.orcid | 0000-0001-7001-0739 | tr_TR |
dc.contributor.orcid | 0000-0002-9381-0410 | tr_TR |
dc.contributor.orcid | 0000-0002-8410-1786 | tr_TR |
dc.contributor.orcid | 0000-0002-6729-7908 | tr_TR |
dc.contributor.researcherid | X-4621-2018 | tr_TR |
dc.contributor.researcherid | AFR-1890-2022 | tr_TR |
dc.contributor.researcherid | A-5608-2019 | tr_TR |
dc.contributor.researcherid | AAG-7012-2021 | tr_TR |
dc.contributor.scopusid | 35344478800 | tr_TR |
dc.contributor.scopusid | 55697616600 | tr_TR |
dc.contributor.scopusid | 7003565902 | tr_TR |
dc.contributor.scopusid | 57194269996 | tr_TR |
dc.contributor.scopusid | 24376085300 | tr_TR |
dc.date.accessioned | 2023-01-11T13:43:20Z | |
dc.date.available | 2023-01-11T13:43:20Z | |
dc.date.issued | 2018-01-16 | |
dc.description.abstract | Lung cancer has a high treatment cost and poor prognosis in comparison to other types of cancers. This work was involved in studying oxidative DNA base damage inhibition. Accordingly, standard carvacrol, thymol, thymoquinone with water and water-methanol extract of thyme (Origanum vulgare L. subsp. hirtum (link.) Ietswaart), thyme oil and thyme water were prepared and investigated for their efficacy to inhibit DNA oxidative damage formed by H2O2 in malignant lung cells (A549). The antioxidant capacity by ABTS assay was 271.73 +/- 11.45 mg trolox equivalent/mL for thyme oil. HPLC analysis was carried out to determine the contents of different thyme extracts, results showing the presence of carvacrol, thymol, protocatechuic acid, caffeic acid, epicatechin and rosmarinic acid in water and water-methanol extracts while only carvacrol and thymol were found in thyme oil and thyme water. After DNA isolation from the cultured cells, the formed oxidative induced DNA damage products were analysed using GC-MS/MS. It was proven that the antioxidants in the cell culture media have succeeded to inhibit oxidative DNA base damage. Thymoquinone was shown to be the best protectant antioxidant among other antioxidants against the formation of oxidative DNA damage, whereas water-methanol extract of thyme was the best among the plant-sourced samples. Thymoquinone and thyme water-methanol extract were investigated for their efficacy on cultured healthy lung cells (BEAS-2B), and it was proven that they are efficient in protection against the oxidation of DNA of healthy lung cells too. | en_US |
dc.identifier.citation | Aybastıer, Ö. vd. (2018). ''Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage''. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 808, 1-9. | en_US |
dc.identifier.endpage | 9 | tr_TR |
dc.identifier.issn | 0027-5107 | |
dc.identifier.issn | 1873-135X | |
dc.identifier.pubmed | 29366947 | tr_TR |
dc.identifier.scopus | 2-s2.0-85040666884 | tr_TR |
dc.identifier.startpage | 1 | tr_TR |
dc.identifier.uri | https://doi.org/10.1016/j.mrfmmm.2018.01.004 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0027510717301483 | |
dc.identifier.uri | http://hdl.handle.net/11452/30408 | |
dc.identifier.volume | 808 | tr_TR |
dc.identifier.wos | 000428342000001 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.bap | 2014/5 | tr_TR |
dc.relation.collaboration | Yurt dışı | tr_TR |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Biotechnology & applied microbiology | en_US |
dc.subject | Genetics & heredity | en_US |
dc.subject | Toxicology | en_US |
dc.subject | Oxidative DNA damage | en_US |
dc.subject | Antioxidant | en_US |
dc.subject | Thyme | en_US |
dc.subject | Lung cancer | en_US |
dc.subject | GC-MS/MS | en_US |
dc.subject | Hydrogen-peroxide | en_US |
dc.subject | Epithelial-cells | en_US |
dc.subject | Cancer patients | en_US |
dc.subject | Repair | en_US |
dc.subject | Corcinoma | en_US |
dc.subject | Phenolics | en_US |
dc.subject | Adducts | en_US |
dc.subject | Stress | en_US |
dc.subject | Risk | en_US |
dc.subject.emtree | Caffeic acid | en_US |
dc.subject.emtree | Carvacrol | en_US |
dc.subject.emtree | Distilled water | en_US |
dc.subject.emtree | DNA base | en_US |
dc.subject.emtree | Epicatechin | en_US |
dc.subject.emtree | Hydrogen peroxide | en_US |
dc.subject.emtree | Methanol | en_US |
dc.subject.emtree | Protocatechuic acid | en_US |
dc.subject.emtree | Rosmarinic acid | en_US |
dc.subject.emtree | Thyme distillate water | en_US |
dc.subject.emtree | Thyme oil | en_US |
dc.subject.emtree | Thymol | en_US |
dc.subject.emtree | Thymoquinone | en_US |
dc.subject.emtree | Thymus vulgaris extract | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | Antioxidant | en_US |
dc.subject.emtree | Plant extract | en_US |
dc.subject.emtree | A-549 cell line | en_US |
dc.subject.emtree | Antioxidant activity | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | BEAS-2B cell line | en_US |
dc.subject.emtree | Cell protection | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | DNA damage | en_US |
dc.subject.emtree | DNA isolation | en_US |
dc.subject.emtree | High performance liquid chromatography | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Human cell | en_US |
dc.subject.emtree | Lung cancer cell line | en_US |
dc.subject.emtree | Mass fragmentography | en_US |
dc.subject.emtree | Oxidative stress | en_US |
dc.subject.emtree | Priority journal | en_US |
dc.subject.emtree | Cell culture | en_US |
dc.subject.emtree | Chemistry | en_US |
dc.subject.emtree | Drug effect | en_US |
dc.subject.emtree | Lung | en_US |
dc.subject.emtree | Lung tumor | en_US |
dc.subject.emtree | Mass fragmentography | en_US |
dc.subject.emtree | Metabolism | en_US |
dc.subject.emtree | Non small cell lung cancer | en_US |
dc.subject.emtree | Oxidation reduction reaction | en_US |
dc.subject.emtree | Oxidative stress | en_US |
dc.subject.emtree | Pathology | en_US |
dc.subject.emtree | Procedures | en_US |
dc.subject.emtree | Thymus (plant) | en_US |
dc.subject.mesh | Antioxidants | en_US |
dc.subject.mesh | Carcinoma, non-small-cell lung | en_US |
dc.subject.mesh | Cells, cultured | en_US |
dc.subject.mesh | DNA damage | en_US |
dc.subject.mesh | Gas chromatography-mass spectrometry | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Lung | en_US |
dc.subject.mesh | Lung neoplasms | en_US |
dc.subject.mesh | Oxidation-reduction | en_US |
dc.subject.mesh | Oxidative stress | en_US |
dc.subject.mesh | Plant extracts | en_US |
dc.subject.mesh | Thymus plant | en_US |
dc.subject.scopus | Biomarkers; DNA Damage; Aptasensors | en_US |
dc.subject.wos | Biotechnology & applied microbiology | en_US |
dc.subject.wos | Genetics & heredity | en_US |
dc.subject.wos | Toxicology | en_US |
dc.title | Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage | en_US |
dc.type | Article | |
dc.wos.quartile | Q4 (Toxicology) | en_US |
dc.wos.quartile | Q3 | en_US |
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