Binary and ternary new water soluble copper(II) complexes of l-tyrosine and substituted 1,10-phenanthrolines: Effect of substitution on DNA interactions and cytotoxicities
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Date
2015-06-15
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Pergamon-Elsevier
Abstract
Binary and ternary water soluble copper(II) complexes - [Cu(nphen)(2)(H2O)](NO3)(2)center dot H2O (1), [Cu(phen)(2)(H2O)](NO3)(2) (2), [Cu(nphen)(L-tyr)(H2O)]NO3 center dot 2H(2)O (3), [Cu(Phen)(tyr)(H2O)] NO3 center dot 2H(2)O (4) - and diquarternary salts of nphen and phen (nphen = 5-nitro-1,10-phenanthroline, phen = 1,10-phenanthroline and tyr = L-tyrosine) have been synthesized and characterized by CHN analysis, H-1 NMR, C-13 NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by thermal denaturation measurements, absorption and emission spectroscopy. The supercoiled pUC19 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by using XTT method. The complexes 1-4 exhibit significant high cytotoxicity with low IC50 values in compared with cisplatin. The effect of the substituents of phen and coordinated amino acid in the above complexes are presented and discussed.
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Keywords
1,10-Phenanthroline, 5-Nitro-1,10-phenanthroline, Copper(II), Cytotoxicity, DNA binding and cleavage, l-Tyrosine, Oxidative cleavage activity, Weak-interactions, Crystal-structure, Amino-acids, Binding, Fluorescence, Derıvatives, Mononuclear, Organotin(IV), Ruthenium(II), Spectroscopy, Amino acids, Binding energy, Complexation, Coordination reactions, Copper, Cytotoxicity, DNA, Electrophoresis, Emission spectroscopy, Nuclear magnetic resonance spectroscopy, Platinum compounds, Single crystals, Synthesis (chemical), Thermoanalysis, X ray diffraction, 1 ,10-phenanthroline, 5-Nitro-1,10-phenanthroline, Absorption and emission spectroscopy, Agarose gel electrophoresis, DNA binding and cleavages, Effect of substitution, l-Tyrosine, Single crystal x-ray diffraction, Copper compounds
Citation
İnci, D. vd. (2015). "Binary and ternary new water soluble copper(II) complexes of l-tyrosine and substituted 1,10-phenanthrolines: Effect of substitution on DNA interactions and cytotoxicities". Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 145, 313-324.