CDP-choline-induced contractions in the mouse gastric fundus through purinoceptors and Rho/Rho-kinase signalling
dc.contributor.author | Güldalı, Özge | |
dc.contributor.author | Büyükafşar, Kansu | |
dc.contributor.buuauthor | Savcı, Vahide | |
dc.contributor.department | Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı. | tr_TR |
dc.contributor.scopusid | 6603687024 | tr_TR |
dc.date.accessioned | 2022-03-28T08:34:38Z | |
dc.date.available | 2022-03-28T08:34:38Z | |
dc.date.issued | 2011-03 | |
dc.description.abstract | Aims: This study aimed to investigate the effects of cytidine-5'-diphosphocholine (CDP-choline), an endogenous lipid precursor, on the reactivity of the mouse gastric fundus and to determine the mechanism(s) mediating its effects. Main methods: Possible contractile effect of CDP-choline (10(-5)-10(-2) M) was investigated in the absence and presence of a muscarinic receptor antagonist, atropine (3 x 10(-6) M), an acetylcholine esterase inhibitor, physostigmine (10(-6) M), a Na+ channel blocker, tetrodotoxin (TTX, 3 x 10(-6) M), a Rho-kinase inhibitor, Y-27632 (10(-5) M), a purinoceptor antagonist, suramin (2 x 10(-4) M), a nitric oxide synthase inhibitor, N-G-nitro-L-arginine (L-NA, 3 x 10(-4) M), a Ca2+ channel blocker, nifedipine (10(-6) M), an alpha(7) nicotinic receptor antagonist, methyllycaconitine citrate (MLA, 10(-6) M) and a G protein (G(i/o)) inhibitor, pertussis toxin (PTX, 2 mu g/ml). The metabolites of CDP-choline, namely choline (10(-4)-10(-2) M), cytidine 5'-triphosphate (CTP, 10(-5)-10(-2) M), cytidine (10(-5)-10(-2) M) and cytidine monophosphate (CMP, 10(-3)-10(-2) M) were also tested. Besides, phosphorylation of MYPT1, which indicates Rho-kinase activity, was also detected. Key findings: COP-choline produced contractions in a concentration-dependent manner. The contractions were not affected by atropine, physostigmine, TTX, PTX, MLA or L-NA. However, Y-27632, suramin or nifedipine partly reduced these contractions. COP-choline increased phosphorylation of MYPT1. Among COP-choline metabolites, cytidine had no contractile effects. However, choline induced considerable contractions, which were sensitive to atropine. CMP and CTP had also contractile activity, comparable to that of COP-choline. Significance: These results suggest that CDP-choline produced contraction through, at least in part, purinoceptors and Rho/Rho-kinase signalling in the mouse gastric fundus. | en_US |
dc.identifier.citation | Güldalı, Ö. vd.(2011). "CDP-choline-induced contractions in the mouse gastric fundus through purinoceptors and Rho/Rho-kinase signalling". Life Sciences, 88(11-12), 473-479. | en_US |
dc.identifier.endpage | 479 | tr_TR |
dc.identifier.issn | 0024-3205 | |
dc.identifier.issn | 1879-0631 | |
dc.identifier.issue | 11-12 | tr_TR |
dc.identifier.pubmed | 21219915 | tr_TR |
dc.identifier.scopus | 2-s2.0-79952454875 | tr_TR |
dc.identifier.startpage | 473 | tr_TR |
dc.identifier.uri | https://doi.org/10.1016/j.lfs.2011.01.002 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S002432051100004X | |
dc.identifier.uri | http://hdl.handle.net/11452/25378 | |
dc.identifier.volume | 88 | tr_TR |
dc.identifier.wos | 000288234400002 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Pergamon-Elsevier Science | en_US |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.journal | Life Sciences | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Research & experimental medicine | en_US |
dc.subject | Pharmacology & pharmacy | en_US |
dc.subject | CDP-choline | en_US |
dc.subject | Gastric fundus | en_US |
dc.subject | Purinoceptors | en_US |
dc.subject | Rho-kinase | en_US |
dc.subject | Y-27632 | en_US |
dc.subject | Rho-Kinase | en_US |
dc.subject | Myosin phosphatase | en_US |
dc.subject | Rat-Brain | en_US |
dc.subject | Receptors | en_US |
dc.subject | Involvement | en_US |
dc.subject | P2y | en_US |
dc.subject | Relaxation | en_US |
dc.subject | Metabolism | en_US |
dc.subject | Activation | en_US |
dc.subject | Expression | en_US |
dc.subject.emtree | 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide | en_US |
dc.subject.emtree | Atropine | en_US |
dc.subject.emtree | Choline | en_US |
dc.subject.emtree | Citicoline | en_US |
dc.subject.emtree | Contractile protein | en_US |
dc.subject.emtree | Cytidine | en_US |
dc.subject.emtree | Cytidine phosphate | en_US |
dc.subject.emtree | Cytidine triphosphate | en_US |
dc.subject.emtree | Methyllycaconitine | en_US |
dc.subject.emtree | Myosin phosphatase target subunit 1 protein | en_US |
dc.subject.emtree | Ng) nitroarginine | en_US |
dc.subject.emtree | Nifedipine | en_US |
dc.subject.emtree | Pertussis toxin | en_US |
dc.subject.emtree | Physostigmine | en_US |
dc.subject.emtree | Purinergic receptor | en_US |
dc.subject.emtree | Rho kinase | en_US |
dc.subject.emtree | Suramin | en_US |
dc.subject.emtree | Tetrodotoxin | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | Animal tissue | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Drug effect | en_US |
dc.subject.emtree | Enzyme activity | en_US |
dc.subject.emtree | In vivo study | en_US |
dc.subject.emtree | Mouse | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | Protein phosphorylation | en_US |
dc.subject.emtree | Signal transduction | en_US |
dc.subject.emtree | Stomach fundus | en_US |
dc.subject.emtree | Stomach muscle | en_US |
dc.subject.emtree | Western blotting | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Blotting, western | en_US |
dc.subject.mesh | Cytidine diphosphate choline | en_US |
dc.subject.mesh | Dose-response relationship, drug | en_US |
dc.subject.mesh | Enzyme inhibitors | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gastric fundus | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, inbred BALB C | en_US |
dc.subject.mesh | Muscle contraction | en_US |
dc.subject.mesh | Muscle, smooth | en_US |
dc.subject.mesh | Purinergic antagonists | en_US |
dc.subject.mesh | Receptors, purinergic | en_US |
dc.subject.mesh | rho-Associated kinases | en_US |
dc.subject.mesh | Signal transduction | en_US |
dc.subject.scopus | Citicoline; Neuroprotective Agents; Glycerylphosphorylcholine | en_US |
dc.subject.wos | Medicine, research & experimental | en_US |
dc.subject.wos | Pharmacology & pharmacy | en_US |
dc.title | CDP-choline-induced contractions in the mouse gastric fundus through purinoceptors and Rho/Rho-kinase signalling | en_US |
dc.type | Article | |
dc.wos.quartile | Q2 | en_US |
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