Publication: Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells
dc.contributor.author | Güney, Gamze Eskiler | |
dc.contributor.author | Özkan, Asuman Deveci | |
dc.contributor.buuauthor | Eryılmaz, Işıl Ezgi | |
dc.contributor.buuauthor | Egeli, Ünal | |
dc.contributor.buuauthor | Cecener, Gülşah | |
dc.contributor.department | Tıp Fakültesi | |
dc.contributor.department | Tıbbi Biyoloji Ana Bilim Dalı | |
dc.contributor.orcid | https://orcid.org/0000-0002-3316-316X | |
dc.contributor.researcherid | GWV-3548-2022 | |
dc.contributor.scopusid | 57189380840 | |
dc.contributor.scopusid | 55665145000 | |
dc.contributor.scopusid | 6508156530 | |
dc.date.accessioned | 2024-01-19T07:39:11Z | |
dc.date.available | 2024-01-19T07:39:11Z | |
dc.date.issued | 2021-07 | |
dc.description.abstract | Background: We evaluated the effect of cabazitaxel (CAB) as a third-line taxane on Toll-like receptor 4 (TLR4)-mediated signaling pathways, especially NF-κB activity, in metastatic castration-resistant prostate cancer (mCRPC) cells. Methods: CAB cytotoxicity was determined by WST-1 assay. To assess the relationship between CAB efficacy and TLR4 signaling pathways, RT-PCR, western blot and immunofluorescence analysis were performed. Additionally, CAB-mediated apoptotic cell death was assessed by Annexin V and RT-PCR analysis. Results: Our results demonstrated that CAB exerted considerably cytotoxic and apoptotic effects on PC-3 mCRPC cells (p < 0.05). CAB treatment altered TLR4 expression level in a dose-dependent manner. Furthermore, 1 nM CAB treatment significantly induced NF-κB activity through p65 nuclear localization and increased the expression level of caspase-3, Bax and p53. Interestingly, total apoptotic cell death and IRF3 protein levels were increased at 5 nM concentration of CAB despite a decrease in the levels of both NF-κB and pro-apoptotic genes. Conclusions: Therefore, NF-κB activity may be a potential target for the efficacy of CAB in mCRPC cells. | |
dc.identifier.citation | Eskiler, G. G. vd. (2021). ''Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells''. Human Experimental Toxicology, 40(7), 1122-1129. | |
dc.identifier.endpage | 1129 | |
dc.identifier.issn | 0960-3271 | |
dc.identifier.issn | 1477-0903 | |
dc.identifier.issue | 7 | |
dc.identifier.pubmed | 33380212 | |
dc.identifier.scopus | 2-s2.0-85098495939 | |
dc.identifier.startpage | 1122 | |
dc.identifier.uri | https://doi.org/10.1177/0960327120984209 | |
dc.identifier.uri | https://journals.sagepub.com/doi/pdf/10.1177/0960327120984209 | |
dc.identifier.uri | https://hdl.handle.net/11452/39180 | |
dc.identifier.volume | 40 | |
dc.identifier.wos | 000636518700001 | |
dc.indexed.scopus | Scopus | |
dc.indexed.wos | SCIE | |
dc.language.iso | en | |
dc.publisher | SAGE Publications | |
dc.relation.bap | (BUAP(T)-2015/4) | |
dc.relation.collaboration | Yurt içi | |
dc.relation.journal | Human and Experimental Toxicology | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Apoptosis | |
dc.subject | Cabazitaxel | |
dc.subject | Metastatic castration-resistant prostate cancer | |
dc.subject | Toxicology | |
dc.subject | Toll-like receptor 4 | |
dc.subject.emtree | Cabazitaxel | |
dc.subject.emtree | Caspase 3 | |
dc.subject.emtree | Immunoglobulin enhancer binding protein | |
dc.subject.emtree | Interferon regulatory factor 3 | |
dc.subject.emtree | Lipocortin 5 | |
dc.subject.emtree | Protein Bax | |
dc.subject.emtree | Synaptotagmin I | |
dc.subject.emtree | Toll like receptor 4 | |
dc.subject.emtree | Antineoplastic agent | |
dc.subject.emtree | Taxoid | |
dc.subject.emtree | Toll like receptor 4 | |
dc.subject.emtree | Antineoplastic activity | |
dc.subject.emtree | Apoptosis | |
dc.subject.emtree | Article | |
dc.subject.emtree | Bax gene | |
dc.subject.emtree | Cas 3 gene | |
dc.subject.emtree | Castration-resistant prostate cancer cell line; cell death | |
dc.subject.emtree | Controlled study | |
dc.subject.emtree | Cytotoxicity | |
dc.subject.emtree | Gene activity | |
dc.subject.emtree | Gene expression | |
dc.subject.emtree | Human | |
dc.subject.emtree | Human cell | |
dc.subject.emtree | Immunofluorescence | |
dc.subject.emtree | IRF3 gene | |
dc.subject.emtree | Metastatic castration resistant prostate cancer | |
dc.subject.emtree | NK kB gene | |
dc.subject.emtree | p65 gene | |
dc.subject.emtree | Real time polymerase chain reaction | |
dc.subject.emtree | Signal transduction | |
dc.subject.emtree | TLR4 gene | |
dc.subject.emtree | Tumor suppressor gene | |
dc.subject.emtree | Western blotting | |
dc.subject.emtree | WST-1 assay | |
dc.subject.emtree | Castration resistant prostate cancer | |
dc.subject.emtree | Cell culture | |
dc.subject.emtree | Cell survival | |
dc.subject.emtree | Drug effect | |
dc.subject.emtree | Male | |
dc.subject.emtree | Pathophysiology | |
dc.subject.emtree | Signal transduction | |
dc.subject.emtree | Treatment outcome | |
dc.subject.mesh | Antineoplastic agents | |
dc.subject.mesh | Apoptosis | |
dc.subject.mesh | Cell survival | |
dc.subject.mesh | Cells | |
dc.subject.mesh | Cultured | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Male | |
dc.subject.mesh | Prostatic neoplasms | |
dc.subject.mesh | Castration-resistant | |
dc.subject.mesh | Signal transduction | |
dc.subject.mesh | Taxoids | |
dc.subject.mesh | Toll-like receptor 4 | |
dc.subject.mesh | Treatment outcome | |
dc.subject.other | NF-k | |
dc.subject.other | B activity | |
dc.subject.scopus | Signal Transduction; CBLB502; Myeloid Differentiation Factor 88 | |
dc.subject.wos | Toxicology | |
dc.title | Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells | |
dc.type | Article | |
dc.wos.quartile | Q2 | |
dspace.entity.type | Publication | |
local.contributor.department | Tıp Fakültesi/Tıbbi Biyoloji Ana Bilim Dalı | |
local.indexed.at | WOS | |
local.indexed.at | Scopus |
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