Publication:
Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells

dc.contributor.authorGüney, Gamze Eskiler
dc.contributor.authorÖzkan, Asuman Deveci
dc.contributor.buuauthorEryılmaz, Işıl Ezgi
dc.contributor.buuauthorEgeli, Ünal
dc.contributor.buuauthorCecener, Gülşah
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıbbi Biyoloji Ana Bilim Dalı
dc.contributor.orcidhttps://orcid.org/0000-0002-3316-316X
dc.contributor.researcheridGWV-3548-2022
dc.contributor.scopusid57189380840
dc.contributor.scopusid55665145000
dc.contributor.scopusid6508156530
dc.date.accessioned2024-01-19T07:39:11Z
dc.date.available2024-01-19T07:39:11Z
dc.date.issued2021-07
dc.description.abstractBackground: We evaluated the effect of cabazitaxel (CAB) as a third-line taxane on Toll-like receptor 4 (TLR4)-mediated signaling pathways, especially NF-κB activity, in metastatic castration-resistant prostate cancer (mCRPC) cells. Methods: CAB cytotoxicity was determined by WST-1 assay. To assess the relationship between CAB efficacy and TLR4 signaling pathways, RT-PCR, western blot and immunofluorescence analysis were performed. Additionally, CAB-mediated apoptotic cell death was assessed by Annexin V and RT-PCR analysis. Results: Our results demonstrated that CAB exerted considerably cytotoxic and apoptotic effects on PC-3 mCRPC cells (p < 0.05). CAB treatment altered TLR4 expression level in a dose-dependent manner. Furthermore, 1 nM CAB treatment significantly induced NF-κB activity through p65 nuclear localization and increased the expression level of caspase-3, Bax and p53. Interestingly, total apoptotic cell death and IRF3 protein levels were increased at 5 nM concentration of CAB despite a decrease in the levels of both NF-κB and pro-apoptotic genes. Conclusions: Therefore, NF-κB activity may be a potential target for the efficacy of CAB in mCRPC cells.
dc.identifier.citationEskiler, G. G. vd. (2021). ''Association between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells''. Human Experimental Toxicology, 40(7), 1122-1129.
dc.identifier.endpage1129
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue7
dc.identifier.pubmed33380212
dc.identifier.scopus2-s2.0-85098495939
dc.identifier.startpage1122
dc.identifier.urihttps://doi.org/10.1177/0960327120984209
dc.identifier.urihttps://journals.sagepub.com/doi/pdf/10.1177/0960327120984209
dc.identifier.urihttps://hdl.handle.net/11452/39180
dc.identifier.volume40
dc.identifier.wos000636518700001
dc.indexed.scopusScopus
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherSAGE Publications
dc.relation.bap(BUAP(T)-2015/4)
dc.relation.collaborationYurt içi
dc.relation.journalHuman and Experimental Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectCabazitaxel
dc.subjectMetastatic castration-resistant prostate cancer
dc.subjectToxicology
dc.subjectToll-like receptor 4
dc.subject.emtreeCabazitaxel
dc.subject.emtreeCaspase 3
dc.subject.emtreeImmunoglobulin enhancer binding protein
dc.subject.emtreeInterferon regulatory factor 3
dc.subject.emtreeLipocortin 5
dc.subject.emtreeProtein Bax
dc.subject.emtreeSynaptotagmin I
dc.subject.emtreeToll like receptor 4
dc.subject.emtreeAntineoplastic agent
dc.subject.emtreeTaxoid
dc.subject.emtreeToll like receptor 4
dc.subject.emtreeAntineoplastic activity
dc.subject.emtreeApoptosis
dc.subject.emtreeArticle
dc.subject.emtreeBax gene
dc.subject.emtreeCas 3 gene
dc.subject.emtreeCastration-resistant prostate cancer cell line; cell death
dc.subject.emtreeControlled study
dc.subject.emtreeCytotoxicity
dc.subject.emtreeGene activity
dc.subject.emtreeGene expression
dc.subject.emtreeHuman
dc.subject.emtreeHuman cell
dc.subject.emtreeImmunofluorescence
dc.subject.emtreeIRF3 gene
dc.subject.emtreeMetastatic castration resistant prostate cancer
dc.subject.emtreeNK kB gene
dc.subject.emtreep65 gene
dc.subject.emtreeReal time polymerase chain reaction
dc.subject.emtreeSignal transduction
dc.subject.emtreeTLR4 gene
dc.subject.emtreeTumor suppressor gene
dc.subject.emtreeWestern blotting
dc.subject.emtreeWST-1 assay
dc.subject.emtreeCastration resistant prostate cancer
dc.subject.emtreeCell culture
dc.subject.emtreeCell survival
dc.subject.emtreeDrug effect
dc.subject.emtreeMale
dc.subject.emtreePathophysiology
dc.subject.emtreeSignal transduction
dc.subject.emtreeTreatment outcome
dc.subject.meshAntineoplastic agents
dc.subject.meshApoptosis
dc.subject.meshCell survival
dc.subject.meshCells
dc.subject.meshCultured
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshProstatic neoplasms
dc.subject.meshCastration-resistant
dc.subject.meshSignal transduction
dc.subject.meshTaxoids
dc.subject.meshToll-like receptor 4
dc.subject.meshTreatment outcome
dc.subject.otherNF-k
dc.subject.otherB activity
dc.subject.scopusSignal Transduction; CBLB502; Myeloid Differentiation Factor 88
dc.subject.wosToxicology
dc.titleAssociation between the anticancer efficacy of cabazitaxel and toll-like receptor 4 mediating signaling pathways in metastatic castration-resistant prostate cancer cells
dc.typeArticle
dc.wos.quartileQ2
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Tıbbi Biyoloji Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus

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