Cellulose monoacetate/polycaprolactone and cellulose monoacetate/polycaprolactam blended nanofibers for protease immobilization

dc.contributor.buuauthorAykut, Yakup
dc.contributor.buuauthorSevgi, Tuba
dc.contributor.buuauthorDemirkan, Elif
dc.contributor.departmentUludağ Üniversitesi/Mühendislik Fakültesi/Tekstil Mühendisliği Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.tr_TR
dc.contributor.orcid0000-0002-7528-9529tr_TR
dc.contributor.researcheridABI-4472-2020tr_TR
dc.contributor.researcheridAAG-7112-2021tr_TR
dc.contributor.scopusid55320835000tr_TR
dc.contributor.scopusid57191880859tr_TR
dc.contributor.scopusid23469245200tr_TR
dc.date.accessioned2023-02-21T11:05:07Z
dc.date.available2023-02-21T11:05:07Z
dc.date.issued2017-06-25
dc.description.abstractEnzymes can be used multiple times when they are immobilized on a support. More enzymes can be immobilized on a surface when nanofibers are used as a supporting surface because the specific surface area increases tremendously. In this regard, polycaprolactam/cellulose monoacetate (PA6/CMA) and polycaprolactone/cellulose monoacetate (PCL/CMA) blended nanofibers (NFs) were prepared via an electrospinning process. Protease enzymes were immobilized on neat PA6, PCL, PA6/CMA, and PCL/CMA nanofibers and glutaraldehyde (GA) activated analogs through the physical adsorption method. The immobilized enzyme activity was measured by using a casein substrate, and the results were compared with free enzyme activity. Among all of the samples, the highest immobilization yield of about 82% was obtained with GA-activated neat PCL NF samples. The best remaining activity of the immobilized enzymes on pure CMA NFs was found to be 59% after seven reuses. Even after nine reuses, enzyme activities are still observed on the CMA NF samples. It was expected that the addition of CMA in PCL and PA6 NFs would increase the reusability number to reach the reusability of CMA NFs, but it was not significantly enhanced. If CMA chains could be mostly collected on the sheath or close to the sheath of the NFs during the electrospinnig process, this target could be achieved.en_US
dc.identifier.citationAykut, Y. vd. (2017). ''Cellulose monoacetate/polycaprolactone and cellulose monoacetate/polycaprolactam blended nanofibers for protease immobilization''. Journal of Applied Polymer Science, 134(44).en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue44tr_TR
dc.identifier.scopus2-s2.0-85024394703tr_TR
dc.identifier.urihttps://doi.org/10.1002/app.45479
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1002/app.45479
dc.identifier.urihttp://hdl.handle.net/11452/31077
dc.identifier.volume134tr_TR
dc.identifier.wos000407788900011tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.bapQUAP(MH) 2014/9tr_TR
dc.relation.journalJournal of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolymer scienceen_US
dc.subjectCellulose and other wood productsen_US
dc.subjectFibersen_US
dc.subjectTextilesen_US
dc.subjectThermoplasticsen_US
dc.subjectNanostructured polymersen_US
dc.subjectPa6 dispersed phaseen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectBiomimetic mineralizationen_US
dc.subjectSelective localizationen_US
dc.subjectMorphology evolutionen_US
dc.subjectAcetate nanofibersen_US
dc.subjectCarbon nanotubesen_US
dc.subjectUltrafine fibersen_US
dc.subjectPP/pa6 blendsen_US
dc.subjectElectrospunen_US
dc.subjectCelluloseen_US
dc.subjectThermoplasticsen_US
dc.subjectWood Productsen_US
dc.subjectPolymersen_US
dc.subjectEnzymesen_US
dc.subjectFibersen_US
dc.subjectNanofibersen_US
dc.subjectReusabilityen_US
dc.subjectTextilesen_US
dc.subjectThermoplasticsen_US
dc.subjectWooden_US
dc.subjectElectrospinning processen_US
dc.subjectImmobilized enzymeen_US
dc.subjectImmobilized enzyme activityen_US
dc.subjectNanostructured polymersen_US
dc.subjectPhysical adsorption methoden_US
dc.subjectPolycaprolactamen_US
dc.subjectProtease enzymeen_US
dc.subjectSupporting surfacesen_US
dc.subjectEnzyme activityen_US
dc.subject.scopusChitosan; Electrospinning; Nanofibersen_US
dc.subject.wosPolymer scienceen_US
dc.titleCellulose monoacetate/polycaprolactone and cellulose monoacetate/polycaprolactam blended nanofibers for protease immobilizationen_US
dc.typeArticle
dc.wos.quartileQ2en_US

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