Response of the cherry rootstock to water stress induced in vitro

No Thumbnail Available

Date

2008-09

Journal Title

Journal ISSN

Volume Title

Publisher

Acad Sciences Czech Republic

Abstract

The in vitro response of sweet cherry (Prunus cerasus x P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.

Description

Keywords

Plant sciences, Antioxidant enzymes, Chlorophyll, Drought injury, Growth, Ion content, Lipid peroxidation, Antioxidant responses, Osmotic adjustment, Growth, Peroxidase, Proline, Drought, Nacl, Accumulation, Chlorophyll, Salinity, Prunus, Prunus avium, Prunus canescens, Prunus cerasus

Citation

Sivritepe, N. vd. (2008). ''Response of the cherry rootstock to water stress induced in vitro''. Biologia Plantarum, 52(3), 573-576.

Collections