First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey

Abstract

Analyzing fingerprints by Restriction Length Polymorphism (RFLP) analysis of complete virus genome which is laborious and time-consuming classifies Bovine Herpervirus-1 (BoHV-1) strains into three subtypes, BoHV-1.1, 1.2a, 1.2b. These subtypes can also being referred according to clinical futures, however, no clear relation was shown. Mostly BoHV-1.2b is associated with genital disease conditions. In this study, BoHV-1 isolate was obtained in a nasal swab sample taken from respiratory tract disease. In this study also, phylogenetic analysis which was targeting UL44 (Glycoprotein C) region of the genome, subtyping was carried out by a recently developed multiplex PCR targeting UL39 and US3 region followed by a RFLP analysis using Hind III enzyme. Also, success of isolation was compared in two continuous cell lines. SFT-R cell line found more susceptible for BoHV-1 field sample isolation than MDBK. The obtained isolate (ID:8640) was serologically undistinguishable from Cooper strain while molecular analysis classified as BoHV-1.2b. Current study reports the first isolation of BoHV-1.2b in Turkey as well as infrequent BoHV-1.2b isolation from clinical case of respiratory illness. Results also highlights the efficiency of PCR based RFLP analysis for easy and quick subtyping but demonstrates the requirement of more investigation to reveal differences based on genetic diversity of BoHV-1 field isolates.