Publication: Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders
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Date
2020-05
Authors
Alçay, Selim
Üstüner, Burcu
Aktar, Ahmet
Mülkpınar, Emine
Duman, Muhammed
Akkaşoğlu, Mustafa
Çetinkaya, Bilge Mehmet
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Journal Title
Journal ISSN
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Publisher
Wiley
Abstract
The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post-thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin-based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 x 10(6) spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA-FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post-thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin-based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.
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Keywords
Post-thaw incubation, Saanen goat, Seminal plasma, Sperm freezing, Soybean lecithin, Ram semen, Egg-yolk, Incubation resilience, Postthaw quality, Liquid storage, Spermatozoa, Antioxidant, Parameters, Endocrinology & metabolism
Citation
Alçay, S. vd. (2020). "Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin-based extenders". Andrologia, 52(4).