Publication:
Application of affinity microspheres for effective SPE cleanup before the determination of sulfamerazine by HPLC

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Date

2018-04-19

Authors

Osman, Bilgen

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Kılıç, Gözde
Tüzmen, Nalan

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Elsevier

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Abstract

This paper describes the application of poly (ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophane methyl ester) [p(EGDMA-MATrp)] microspheres as an affinity sorbent for the SPE (solid phase extraction) cleanup of sulfamerazine (SMR) from food samples of animal origin before high performance liquid chromatography (HPLC) analysis. The microspheres were prepared by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl-L-tryptophane methyl ester (MATrp) as a crosslinker and a monomer, respectively. Various parameters affecting the SPE cleanup efficiency of the p(EGDMA-MATrp) microspheres (contact time, pH, initial SMR concentration, ionic strength etc.) were optimized. Under the optimized conditions, maximum adsorption capacity was found to be 8.55 +/- 0.44 mg/g sorbent at pH 5.0. 90% of the adsorbed SMR was desorbed by using ACN:MeOH (5:5) mixture as a desorption agent. Applicability of the microspheres for the SPE cleanup of SMR residues in the food samples such as egg and milk with HPLC was also determined. It was demonstrated that the prepared p(EGDMA-MATrp) microspheres could be repeatedly applied for SPE cleanup of sulfamerazine before chromatographic analysis. Also, the recoveries of SMR in milk and egg samples were reasonably satisfactory and in the range of 71 to 90%.

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Keywords

Materials science, Affinity microspheres, Antibiotic, Sulfamerazine, SPE cleanup, HPLC, Solid-phase extraction, Performance liquid-chromatography, Personal care products, Aqueous-solution, Mass-spectrometry, Malachite-green, Estrogenic chemicals, Antibiotic-residues, Confirmatory method, Activated carbon, Amino acids, Antibiotics, Chromatographic analysis, Desorption, Esters, Ethylene, Ethylene glycol, Ionic strength, High performance liquid chromatography, Phase separation, Polyols, Adsorption capacities, Ethylene glycol dimethacrylate, HPLC, Optimized conditions, Solid-phase extraction, SPE clean-up, Sulfamerazine, Suspension polymerization, Microspheres

Citation

Kılıç, G. vd. (2018). ''Application of affinity microspheres for effective SPE cleanup before the determination of sulfamerazine by HPLC''. Materials Science and Engineering C., 91, 55-63.

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