Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr

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dc.contributor.authorKhider, Mohammed
dc.contributor.buuauthorBüyükcangaz, Esra
dc.contributor.buuauthorSen, Aysin
dc.contributor.buuauthorCengiz, Murat
dc.contributor.buuauthorSonal, Songül
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi.tr_TR
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.tr_TR
dc.contributor.orcid0000-0003-4337-577Xtr_TR
dc.contributor.orcid0000-0002-9212-8743tr_TR
dc.contributor.orcid0000-0001-7601-7640tr_TR
dc.contributor.orcid0000-0001-9018-1842tr_TR
dc.date.accessioned2020-12-22T10:49:16Z
dc.date.available2020-12-22T10:49:16Z
dc.date.issued2019-11-10
dc.description.abstractThis research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials.en_US
dc.identifier.citationBüyükcangaz, E. vd. (2019). "Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 38(2), 88-94.tr_TR
dc.identifier.endpage94tr_TR
dc.identifier.issn1301-3173
dc.identifier.issue2tr_TR
dc.identifier.startpage88tr_TR
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/891633
dc.identifier.urihttp://hdl.handle.net/11452/14551
dc.identifier.volume38tr_TR
dc.language.isoenen_US
dc.publisherBursa Uludağ Üniversitesitr_TR
dc.relation.bapProje No: KUAP (V) 2014/43tr_TR
dc.relation.collaborationYurtdışı.tr_TR
dc.relation.journalVeteriner Hekimlikte Araştırma Dergisi / Journal of Research in Veterinary Medicinetr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobial susceptibilityen_US
dc.subjectE. colien_US
dc.subjectSTECen_US
dc.subjectPCRen_US
dc.subjectEPECen_US
dc.subjectETECen_US
dc.titleDetection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcren_US
dc.typeArticleen_US

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2019 Cilt 38 Sayı 2