Real-time PCR culture and serology for the diagnosis of mycoplasma gallisepticum in chicken breeder flocks

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dc.contributor.buuauthorKahya, Serpil
dc.contributor.buuauthorTemelli, Seran
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Öncesi Bilimler Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Veterinerlik Fakültesi/Gıda Hijyeni ve Teknolojileri Bölümü.tr_TR
dc.contributor.researcheridAAI-1092-2021tr_TR
dc.contributor.researcheridAAH-2842-2021tr_TR
dc.contributor.researcheridAAI-1101-2021tr_TR
dc.contributor.researcheridE-3867-2010tr_TR
dc.contributor.scopusid35368679200tr_TR
dc.contributor.scopusid6506404118tr_TR
dc.contributor.scopusid6602558950tr_TR
dc.contributor.scopusid6601971539tr_TR
dc.date.accessioned2022-04-11T13:24:09Z
dc.date.available2022-04-11T13:24:09Z
dc.date.issued2010-08-26
dc.description.abstractThis study aimed to compare a real-time PCR (rPCR) test with improved detection limit to serology and culture for the detection of Mycoplasma gallisepticum (MG) infection in chicken breeder flocks. Six hundred and forty-six blood and tracheal swab samples belonging to 31 grandparent chicken breeder flocks were tested by rPCR. The detection limit of rPCR was 0.9 pg mu l(-1), with pure MG S6 strain DNA and 100 colony forming units (CFUs) ml(-1), where both pure culture and tracheal swabs were artificially spiked with the same strain. The seropositive flock rate based on both MG RPA and HI were calculated as 48.4% (15/ 31) and 32.3% (10/31), respectively, while culture- and rPCR-positive flock rates were 16.1% (5/31) and 29.0% (9/31), respectively. On flock-based analysis, culture method detected 5 out of 10 MG seropositive flocks (sensitivity 50%, specificity 100%), whereas rPCR detected 8 out of 10 flocks (sensitivity 80%, specificity 95%). Agreements between serology and culture, and serology and rPCR were 83.9% and 90.3%, respectively. On individual sample-based analysis, culture method detected 26 out of 78 MG seropositive chicken (sensitivity 33%, specificity 100%), whereas rPCR detected 51 out of 78 MG seropositive chickens (sensitivity 65%, specificity 96%). There was 91.9% and 91.4% agreement between serology and culture, and serology and rPCR, respectively. Results of this study indicate that the rPCR with improved in vitro detection limit could detect MG in seropositive chicken flocks. Therefore, we advise the use of rPCR and/or culture for confirmation of serology results obtained from screening MG infection in chicken flocks.en_US
dc.identifier.citationKahya, S. vd. (2010). "Real-time PCR culture and serology for the diagnosis of mycoplasma gallisepticum in chicken breeder flocks". Veterinary Microbiology, 144(3-4), 319-324.en_US
dc.identifier.endpage324tr_TR
dc.identifier.issn0378-1135
dc.identifier.issn1873-2542
dc.identifier.issue3-4tr_TR
dc.identifier.pubmed20149561tr_TR
dc.identifier.scopus2-s2.0-77955572600tr_TR
dc.identifier.startpage319tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.vetmic.2010.01.012
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378113510000489
dc.identifier.urihttp://hdl.handle.net/11452/25707
dc.identifier.volume144tr_TR
dc.identifier.wos000281358600008tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.bap2006/29tr_TR
dc.relation.journalVeterinary Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMycoplasma gallisepticumen_US
dc.subjectReal-time PCRen_US
dc.subjectChickenen_US
dc.subjectPolymerase-chain-reactionen_US
dc.subjectAvian mycoplasmasen_US
dc.subjectSynoviaeen_US
dc.subjectStrainen_US
dc.subjectAssayen_US
dc.subjectMicrobiologyen_US
dc.subjectVeterinary sciencesen_US
dc.subjectGallus gallusen_US
dc.subjectMycoplasma gallisepticumen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBacterial strainen_US
dc.subject.emtreeBacterium cultureen_US
dc.subject.emtreeBacterium detectionen_US
dc.subject.emtreeChickenen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeHerden_US
dc.subject.emtreeMycoplasma gallisepticumen_US
dc.subject.emtreeMycoplasma gallisepticum infectionen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeReal time polymerase chain reactionen_US
dc.subject.emtreeSerologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBreedingen_US
dc.subject.meshChickensen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMycoplasma gallisepticumen_US
dc.subject.meshMycoplasma infectionsen_US
dc.subject.meshPolymerase chain reactionen_US
dc.subject.meshPoultry diseasesen_US
dc.subject.meshSensitivity and specificityen_US
dc.subject.meshSerologic testsen_US
dc.subject.scopusMycoplasma Synoviae; Carpodacus Mexicanus; Poultry Industryen_US
dc.subject.wosMicrobiologyen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleReal-time PCR culture and serology for the diagnosis of mycoplasma gallisepticum in chicken breeder flocksen_US
dc.typeArticle
dc.wos.quartileQ1 (Veterinary sciences)en_US
dc.wos.quartileQ2en_US

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