Importance of glycogen phosphorylase isoenzyme BB, total sialic acid, oxidant and antioxidant parameters in the noninvasive assessment of thrombolytic therapy

Abstract

Objective: The aim of this study was to investigate the effect of thrombolytic therapy on oxidant and antioxidant parameters and glycogen phosphorylase isoenzyme BB (GPBB) concentrations in patients with acute myocardial infarction (AMI). Material and Method: This was a prospective study at an urban academic hospital. We enrolled non-traumatized patients arriving at the emergency department within 3 hours after the onset of chest pain suggestive of AMI. The diagnosis of AMI was established according to clinical features, electrocardiographic findings and increased biochemistry cardiac marker levels. The study was carried out on a total of 75 patients with AMI (50 patients received thrombolytic therapy and 25 patients did not receive thrombolytic therapy), and 45 controls. Blood samples were collected before, and, 2 and 24 hours after tissue-type plasminogen activator therapy Serum malondialdehyde (MDA) and vitamin E were analyzed by high-performance liquid chromatography. Serum total sialic acid and other oxidant and antioxidant parameters were studied spectrophotometrically. Determination of GPBB concentration is based on immunoenzymometric assay. Results: Successful reperfusion was followed by increased serum MDA, protein carbonyls, total sialic acid and GPBB concentrations and by decreased antioxidant vitamins and enzymes. Conclusion: These findings indicate the significance free radical generation processes in reperfusion injury in AMI patients, and suggest the potential involvement of antioxidants in the management of AMI treated by thrombolysis. Also, these parameters especially GPBB can be used for the noninvasive assessment of the effectiveness of thrombolytic therapy.