Methods for lipase immobilization and their use for biodiesel production from vegetable oil
No Thumbnail Available
Date
2014-12-03
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis Inc
Abstract
In the present work, two different lipases (triacylglycerol hydrolase, EC 3.1.1.3), Lipozyme TL-100L and Novozyme 388, were immobilized onto three different low-cost supports using both adsorption and covalent method: celite 545, silica gel, and styrene-divinylbenzene copolymer. The maximum immobilization yield was obtained as 79.0% for Lipozyme TL-100L and the highest specific activity was 6.5 U/mg protein for Novozym 388. The properties of the support and immobilized derivatives were characterized by Fourier transform infrared spectroscopy. Maximum methyl esters yield was obtained as 98.3%. The lipases, which are immobilized by covalently, proved to be stable after even 10 repeated reuses.
Description
Keywords
Alcoholysis, Environmental sciences & ecology, Biodiesel, Energy & fuels. Engineering, Lipase immobilization, Triacylglycerol hydrolase, Lipozyme tl-100l, Lipases, Novozyme 388, Styrene-divinylbenzene copolymers, Transesterification, Specific activity, Biodiesel, Lipozyme, Fourier transform infrared spectroscopy, Lipase immobilization, Silica gel, Biodiesel production, Styrene, Transesterification, Alcoholysis
Citation
Yücel, Y. vd. (2014). "Methods for lipase immobilization and their use for biodiesel production from vegetable oil". Energy Sources, Part A: Recovery, Utilization and Environmental Effects, 36(11), 1203-1211.