The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

dc.contributor.authorTezcan, Gülçin
dc.contributor.authorYılmaz, Abdullah
dc.contributor.buuauthorBudak, Ferah
dc.contributor.buuauthorBal, Salih Haldun
dc.contributor.buuauthorAkalın, Emin Halis
dc.contributor.buuauthorHız, Pınar
dc.contributor.buuauthorOral, Haluk Barbaros
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.tr_TR
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-0463-6818tr_TR
dc.contributor.researcheridF-4657-2014
dc.contributor.researcheridAAU-8952-2020
dc.contributor.researcheridK-7285-2012
dc.contributor.researcheridHJY-9001-2023
dc.contributor.researcheridEZA-7505-2022
dc.contributor.scopusid6701913697tr_TR
dc.contributor.scopusid57191480128tr_TR
dc.contributor.scopusid57202649131tr_TR
dc.contributor.scopusid57202644074tr_TR
dc.contributor.scopusid7004498001tr_TR
dc.date.accessioned2024-03-27T11:52:10Z
dc.date.available2024-03-27T11:52:10Z
dc.date.issued2018-05-23
dc.description.abstractBrucellosis is a serious infectious disease that continues to be a significant cause of morbidity worldwide and across all ages. Despite early diagnosis and treatment, 10-30% of patients develop chronic brucellosis. Although there have been recent advances in our knowledge of Brucella virulence factors and hosts' immune response to the infection, there is a lack of clear data regarding how the infection bypasses the immune system and becomes chronic. The present study investigated immunological factors and their roles in the transition of brucellosis from an acute to a chronic infection in CD4+T cells. CD4+T cells sorted from peripheral blood samples of patients with acute or chronic brucellosis and healthy controls using flow cytometry as well as more than 2000 miRNAs were screened using the GeneSpring GX (Agilent) 13.0 miRNA microarray software and were validated using reverse transcription polymerase chain reaction (RT-qPCR). Compared to acute cases, the expression levels of 28 miRNAs were significantly altered in chronic cases. Apart from one miRNA (miR-4649-3p), 27 miRNAs were not expressed in the acute cases (p <0.05, fold change> 2). According to KEGG pathway analysis, these miRNAs are involved in the regulation of target genes that were previously involved in the MAPK signalling pathway, regulation of the actin cytoskeleton, endocytosis, and protein processing in the endoplasmic reticulum. This indicates the potential role of these miRNAs in the development of chronic brucellosis. We suggest that these miRNAs can be used as markers to determine the transition of the disease into chronicity. This is the first study of miRNA expression that analyses human CD4+T cells to clarify the mechanism of chronicity in brucellosis.en_US
dc.identifier.citationBudak, F. vd. (2018). ''The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity''. PLoS One, 13(6).en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0198659en_US
dc.identifier.issn1932-6203
dc.identifier.issue6tr_TR
dc.identifier.pubmed29897958tr_TR
dc.identifier.scopus2-s2.0-85048962569tr_TR
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198659en_US
dc.identifier.urihttps://hdl.handle.net/11452/40639
dc.identifier.volume13tr_TR
dc.identifier.wos000435090700064tr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.collaborationYurt içitr_TR
dc.relation.journalPLoS Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitakSBAG-111S183tr_TR
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectScience & technology - other topicsen_US
dc.subjectLymph-node metastasisen_US
dc.subjectİnhibits proliferationen_US
dc.subjectMirna expressionen_US
dc.subjectOvarian-canceren_US
dc.subjectT-cellsen_US
dc.subjectTargetsen_US
dc.subjectGrowthen_US
dc.subjectCarcinomaen_US
dc.subjectReceptoren_US
dc.subjectPromotesen_US
dc.subject.emtreeMicroRNAen_US
dc.subject.emtreeActin filamenten_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBlood samplingen_US
dc.subject.emtreeBrucellosisen_US
dc.subject.emtreeCD4+ T lymphocyteen_US
dc.subject.emtreeCell selectionen_US
dc.subject.emtreeChronicityen_US
dc.subject.emtreeClinical articleen_US
dc.subject.emtreeCohort analysisen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeData analysis softwareen_US
dc.subject.emtreeEndocytosisen_US
dc.subject.emtreeEndoplasmic reticulumen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeFlow cytometryen_US
dc.subject.emtreeGene controlen_US
dc.subject.emtreeGene expressionen_US
dc.subject.emtreeGene targetingen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeImmune responseen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeProtein processingen_US
dc.subject.emtreeReverse transcription polymerase chain reactionen_US
dc.subject.emtreeSignal transductionen_US
dc.subject.emtreeAcute diseaseen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeBrucellosisen_US
dc.subject.emtreeCase control studyen_US
dc.subject.emtreeChronic diseaseen_US
dc.subject.emtreeCytologyen_US
dc.subject.emtreeDNA microarrayen_US
dc.subject.emtreeGeneticsen_US
dc.subject.emtreeMAPK signalingen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreeMiddle ageden_US
dc.subject.emtreeMononuclear cellen_US
dc.subject.emtreePathologyen_US
dc.subject.emtreeTranslational protein modificationen_US
dc.subject.meshActin cytoskeletonen_US
dc.subject.meshAcute diseaseen_US
dc.subject.meshAdulten_US
dc.subject.meshBrucellosisen_US
dc.subject.meshCase-control studiesen_US
dc.subject.meshCD4-positive T-lymphocytesen_US
dc.subject.meshChronic diseaseen_US
dc.subject.meshEndocytosisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes, mononuclearen_US
dc.subject.meshMaleen_US
dc.subject.meshMAP kinase signaling systemen_US
dc.subject.meshMicroRNAsen_US
dc.subject.meshMiddle ageden_US
dc.subject.meshOligonucleotide array sequence analysisen_US
dc.subject.meshProtein modification, translationalen_US
dc.subject.scopusAnimals; Zoonosis; Bovine Brucellosisen_US
dc.subject.wosMultidisciplinary sciencesen_US
dc.titleThe microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicityen_US
dc.typeArticleen_US
dc.wos.quartileQ2 (Multidisciplinary sciences)en_US

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