The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity
dc.contributor.author | Tezcan, Gülçin | |
dc.contributor.author | Yılmaz, Abdullah | |
dc.contributor.buuauthor | Budak, Ferah | |
dc.contributor.buuauthor | Bal, Salih Haldun | |
dc.contributor.buuauthor | Akalın, Emin Halis | |
dc.contributor.buuauthor | Hız, Pınar | |
dc.contributor.buuauthor | Oral, Haluk Barbaros | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı. | tr_TR |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı. | tr_TR |
dc.contributor.orcid | 0000-0003-0463-6818 | tr_TR |
dc.contributor.researcherid | F-4657-2014 | |
dc.contributor.researcherid | AAU-8952-2020 | |
dc.contributor.researcherid | K-7285-2012 | |
dc.contributor.researcherid | HJY-9001-2023 | |
dc.contributor.researcherid | EZA-7505-2022 | |
dc.contributor.scopusid | 6701913697 | tr_TR |
dc.contributor.scopusid | 57191480128 | tr_TR |
dc.contributor.scopusid | 57202649131 | tr_TR |
dc.contributor.scopusid | 57202644074 | tr_TR |
dc.contributor.scopusid | 7004498001 | tr_TR |
dc.date.accessioned | 2024-03-27T11:52:10Z | |
dc.date.available | 2024-03-27T11:52:10Z | |
dc.date.issued | 2018-05-23 | |
dc.description.abstract | Brucellosis is a serious infectious disease that continues to be a significant cause of morbidity worldwide and across all ages. Despite early diagnosis and treatment, 10-30% of patients develop chronic brucellosis. Although there have been recent advances in our knowledge of Brucella virulence factors and hosts' immune response to the infection, there is a lack of clear data regarding how the infection bypasses the immune system and becomes chronic. The present study investigated immunological factors and their roles in the transition of brucellosis from an acute to a chronic infection in CD4+T cells. CD4+T cells sorted from peripheral blood samples of patients with acute or chronic brucellosis and healthy controls using flow cytometry as well as more than 2000 miRNAs were screened using the GeneSpring GX (Agilent) 13.0 miRNA microarray software and were validated using reverse transcription polymerase chain reaction (RT-qPCR). Compared to acute cases, the expression levels of 28 miRNAs were significantly altered in chronic cases. Apart from one miRNA (miR-4649-3p), 27 miRNAs were not expressed in the acute cases (p <0.05, fold change> 2). According to KEGG pathway analysis, these miRNAs are involved in the regulation of target genes that were previously involved in the MAPK signalling pathway, regulation of the actin cytoskeleton, endocytosis, and protein processing in the endoplasmic reticulum. This indicates the potential role of these miRNAs in the development of chronic brucellosis. We suggest that these miRNAs can be used as markers to determine the transition of the disease into chronicity. This is the first study of miRNA expression that analyses human CD4+T cells to clarify the mechanism of chronicity in brucellosis. | en_US |
dc.identifier.citation | Budak, F. vd. (2018). ''The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity''. PLoS One, 13(6). | en_US |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0198659 | en_US |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issue | 6 | tr_TR |
dc.identifier.pubmed | 29897958 | tr_TR |
dc.identifier.scopus | 2-s2.0-85048962569 | tr_TR |
dc.identifier.uri | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198659 | en_US |
dc.identifier.uri | https://hdl.handle.net/11452/40639 | |
dc.identifier.volume | 13 | tr_TR |
dc.identifier.wos | 000435090700064 | tr_TR |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.collaboration | Yurt dışı | tr_TR |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.journal | PLoS One | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.relation.tubitak | SBAG-111S183 | tr_TR |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Science & technology - other topics | en_US |
dc.subject | Lymph-node metastasis | en_US |
dc.subject | İnhibits proliferation | en_US |
dc.subject | Mirna expression | en_US |
dc.subject | Ovarian-cancer | en_US |
dc.subject | T-cells | en_US |
dc.subject | Targets | en_US |
dc.subject | Growth | en_US |
dc.subject | Carcinoma | en_US |
dc.subject | Receptor | en_US |
dc.subject | Promotes | en_US |
dc.subject.emtree | MicroRNA | en_US |
dc.subject.emtree | Actin filament | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Blood sampling | en_US |
dc.subject.emtree | Brucellosis | en_US |
dc.subject.emtree | CD4+ T lymphocyte | en_US |
dc.subject.emtree | Cell selection | en_US |
dc.subject.emtree | Chronicity | en_US |
dc.subject.emtree | Clinical article | en_US |
dc.subject.emtree | Cohort analysis | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Data analysis software | en_US |
dc.subject.emtree | Endocytosis | en_US |
dc.subject.emtree | Endoplasmic reticulum | en_US |
dc.subject.emtree | Female | en_US |
dc.subject.emtree | Flow cytometry | en_US |
dc.subject.emtree | Gene control | en_US |
dc.subject.emtree | Gene expression | en_US |
dc.subject.emtree | Gene targeting | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Human cell | en_US |
dc.subject.emtree | Immune response | en_US |
dc.subject.emtree | Male | en_US |
dc.subject.emtree | Protein processing | en_US |
dc.subject.emtree | Reverse transcription polymerase chain reaction | en_US |
dc.subject.emtree | Signal transduction | en_US |
dc.subject.emtree | Acute disease | en_US |
dc.subject.emtree | Adult | en_US |
dc.subject.emtree | Brucellosis | en_US |
dc.subject.emtree | Case control study | en_US |
dc.subject.emtree | Chronic disease | en_US |
dc.subject.emtree | Cytology | en_US |
dc.subject.emtree | DNA microarray | en_US |
dc.subject.emtree | Genetics | en_US |
dc.subject.emtree | MAPK signaling | en_US |
dc.subject.emtree | Metabolism | en_US |
dc.subject.emtree | Middle aged | en_US |
dc.subject.emtree | Mononuclear cell | en_US |
dc.subject.emtree | Pathology | en_US |
dc.subject.emtree | Translational protein modification | en_US |
dc.subject.mesh | Actin cytoskeleton | en_US |
dc.subject.mesh | Acute disease | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Brucellosis | en_US |
dc.subject.mesh | Case-control studies | en_US |
dc.subject.mesh | CD4-positive T-lymphocytes | en_US |
dc.subject.mesh | Chronic disease | en_US |
dc.subject.mesh | Endocytosis | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Leukocytes, mononuclear | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | MAP kinase signaling system | en_US |
dc.subject.mesh | MicroRNAs | en_US |
dc.subject.mesh | Middle aged | en_US |
dc.subject.mesh | Oligonucleotide array sequence analysis | en_US |
dc.subject.mesh | Protein modification, translational | en_US |
dc.subject.scopus | Animals; Zoonosis; Bovine Brucellosis | en_US |
dc.subject.wos | Multidisciplinary sciences | en_US |
dc.title | The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity | en_US |
dc.type | Article | en_US |
dc.wos.quartile | Q2 (Multidisciplinary sciences) | en_US |