Publication:
Immobilization of candida antarctica lipase a on chitosan beads for the production of fatty acid methyl ester from waste frying oil

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Date

2011-02-25

Authors

Aybastıer, Önder
Demir, Cevdet

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Taylor & Francis Inc

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Abstract

Candida antarctica lipase A was immobilized by covalent binding onto chitosan beads, and these beads were used for production of fatty acid methyl ester from waste frying canola oil. The optimum immobilization conditions were determined as enzyme concentration of 12% (v/v) and immobilization time of 24 h. Transesterification reaction was performed using enzymatic catalysis for the production of fatty acid methyl ester from waste frying oil. The results indicate that 60% yield of fatty acid methyl ester was obtained under optimum immobilization conditions. Operational stability was determined with immobilized lipase and it indicated that 74% residual activity occurred after using repeatedly for five consecutive batches of 24 h each.

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Keywords

Candida antarctica, Environmental sciences & ecology, Chitosan, Energy & fuels, Fatty acid methyl ester, Selectivity, Immobilization, Engineering, Waste frying oil, Transesterification, Covalent immobilization, Hydrogen-peroxide, Biodiesel production, Enzymatic conversion, Rugosa lipase, Horseradish-peroxidase, Fuel, Candida, Chitosan, Esters, Enzyme immobilization, Isomers, Yeast, Candida antarctica lipase A, Enzymatic catalysis, Transesterification reaction, Enzyme concentrations, Operational stability, Fatty acid methyl ester, Immobilized lipase, Immobilization conditions

Citation

Aybastıer, Ö. ve Demir, C. (2011). "Immobilization of candida antarctica lipase a on chitosan beads for the production of fatty acid methyl ester from waste frying oil". Energy Sources, Part A: Recovery, Utilization and Environmental Effects, 36(21), 2313-2319.

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