Publication:
A simple and rapid microwave-assisted hematoxylin and eosin staining method using 1,1,1 trichloroethane as a dewaxing and a clearing agent

dc.contributor.buuauthorTemel, Şehime
dc.contributor.buuauthorNoyan, Sema
dc.contributor.buuauthorÇavuşoğlu, İlkin
dc.contributor.buuauthorKahveci, Zeynep
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıbbi Genetik Ana Bilim Dalı
dc.contributor.scopusid6507885442tr_TR
dc.contributor.scopusid6603293246tr_TR
dc.contributor.scopusid6602910240tr_TR
dc.contributor.scopusid6603395784tr_TR
dc.date.accessioned2021-09-09T13:40:48Z
dc.date.available2021-09-09T13:40:48Z
dc.date.issued2005-05
dc.description.abstractThe use and practicability of microwave-assisted staining procedures in routine histopathology has been well established for more than 17 years. In the study reported here, we aimed to examine an alternative approach that would shorten the duration of dewaxing and clearing steps of hematoxylin and eosin (H & E) staining of paraffin sections by using a microwave oven. Although xylene is one of the most popular dewaxing and clearing agents, its flammability restricts its use in a microwave oven; thus we preferred 1,1,1 trichloroethane, which is not flammable, as the dewaxing and clearing agent in the present study. In Group I and Group II (control groups), intestine was processed with xylene and 1,1,1 trichloroethane, respectively The sections then were stained with H & E according to the conventional staining protocol at room temperature and subdivided into two groups according to the duration of dewaxing and clearing in xylene. In Groups III and IV (experimental groups) similar tissues were processed with xylene and 1,1,1 trichloroethane, respectively; however, sections from these groups were divided into four subgroups to study the period required for dewaxing and clearing in 1,1,1 trichloroethane, then stained with H & E in the microwave oven at 360 W for 30 sec. Our conventional H & E staining procedure, which includes dewaxing, staining and clearing of sections, requires approximately 90 min, while our method using 1,1,1 trichloroethane and microwave heating required only 2 min. Our alternative method for H & E staining not only reduced the procedure time significantly, but also yielded staining quality equal or superior to those stained the conventional way. Our results suggest that 1,1,1 trichloroethane can be used effectively and safely as a dewaxing and clearing agent for H & E staining in a microwave oven.en_US
dc.identifier.citationTemel, S. vd. (2005). "A simple and rapid microwave-assisted hematoxylin and eosin staining method using 1,1,1 trichloroethane as a dewaxing and a clearing agent". Biotechnic & Histochemistry, 80(3-4), 123-132.en_US
dc.identifier.endpage132tr_TR
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue3-4tr_TR
dc.identifier.pubmed16298897tr_TR
dc.identifier.scopus2-s2.0-29044431956tr_TR
dc.identifier.startpage123tr_TR
dc.identifier.urihttps://doi.org/10.1080/10520290500303190
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/10520290500303190
dc.identifier.urihttp://hdl.handle.net/11452/21832
dc.identifier.volume80tr_TR
dc.identifier.wos000233926100003
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.journalBiotechnic & Histochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClearingen_US
dc.subjectDewaxingen_US
dc.subjectH & Een_US
dc.subjectMicrowaveen_US
dc.subject1,1,1 trichloroethaneen_US
dc.subjectAccelerated proceduresen_US
dc.subjectFixationen_US
dc.subjectSubstituesen_US
dc.subjectIrradiationen_US
dc.subjectArtifactsen_US
dc.subjectHistologyen_US
dc.subjectTissueen_US
dc.subjectCellsen_US
dc.subjectSaferen_US
dc.subjectOvenen_US
dc.subjectBiotechnology & applied microbiologyen_US
dc.subjectCell biologyen_US
dc.subjectAnimaliaen_US
dc.subject.emtree1,1,1 trichloroethaneen_US
dc.subject.emtreeEosinen_US
dc.subject.emtreeHematoxylinen_US
dc.subject.emtreeXyleneen_US
dc.subject.meshAnimal tissueen_US
dc.subject.meshCell structureen_US
dc.subject.meshChemical proceduresen_US
dc.subject.meshControlled studyen_US
dc.subject.meshHistologyen_US
dc.subject.meshIntermethod comparisonen_US
dc.subject.meshMicrowave radiationen_US
dc.subject.meshNonhumanen_US
dc.subject.meshRaten_US
dc.subject.meshRoom temperatureen_US
dc.subject.meshStainingen_US
dc.subject.meshStatistical analysisen_US
dc.subject.meshStatistical significanceen_US
dc.subject.meshTissue preparationen_US
dc.subject.scopusFixatives; Tissue Fixation; Immunohistochemistryen_US
dc.subject.wosBiotechnology & applied microbiologyen_US
dc.subject.wosCell biologyen_US
dc.titleA simple and rapid microwave-assisted hematoxylin and eosin staining method using 1,1,1 trichloroethane as a dewaxing and a clearing agenten_US
dc.typeArticle
dc.wos.quartileQ4en_US
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Histoloji ve Embriyoloji Ana Bilim Dalıtr_TR
local.contributor.departmentTıp Fakültesi/Tıbbi Genetik Ana Bilim Dalıtr_TR

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