İkili iskelet boyamalarında mikrodalga ışınımının kullanılması
Date
2000
Authors
Sunay, F. Bahar
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
İkili iskelet boyamaları, kimyasal maddelerin, iskelet sistemi üzerindeki olası teratojenik etkilerinin araştırılmasında kullanılan yöntemlerdir. Bu incelemelerde uygun istatistiksel değerlendirmenin yapılabilmesi için, çok sayıda deneğin kullanılması zorunludur. Bu nedenle seçilen metodun güvenilir ve kalıcı sonuçlar veren bir yöntem olmasının yanında, kısa sürede tamamlanması da son derece önemlidir. Sık kullanılan ikili iskelet boyamalarından biri Inouye'un metodudur. Bu çalışmanın amacı, Inouye'un metodunda 48-72 saat olan boyama aşamasını mikrodalga ışınımından yararlanarak kısaltmaktır. Çalışmada 32 adet bir günlük Swiss albino cinsi fareler kullanıldı. Grup I'deki denekler (n=12) Inouye'un metodunda tanımladığı şekilde, konvansiyonel etüvde 37°C'de 72 saat süreyle boyandı. Grup II'deki denekler (n=12) mikrodalga fırında, 40.47±4.8°C'de 8 saat süreyle boyandı. Grup III'deki denekler (n=12) ise 48°C'de 8 saat süreyle boyandı. Her üç grupta yer alan denekler boyanmanın kalitesi ve herhangi bir malformasyonu belirlemede yeterliliği açısından incelendiğinde, Grup I ve Grup II arasında istatistikselolarak anlamlı bir fark olmadığı, ancak Grup lII'ün diğer iki grup ile karşılaştırıldığında anlamlı olarak daha kötü boyandığı izlendi. Inouye'un metodunda 72 saat süren boyama aşaması ise, boyama işlemi mikrodalga fırında gerçekleştirildiğinde 8 saatte tamamlandı. ikili iskelet boyamalarında işlemin süresini kısaitmada mikrodalga ışınımından yararlanılabileceği sonucuna varıldı.
Double skeletal staining methods are used to investigate the probable teratogenic effects of chemical substances on the skeletal system. In these studies, in order to perform appropriate statistical evaluation, the number of subjects should be large enough. Therefore, besides being reliable and permanent, the selected method should also be a short one, which will be completed in a short time. One mostly used double skeletal staining method is the Inouye's method. The aim of this study is to reduce the staining time of the Inouye's method, which originally is 48-72 hours, by the use of microwave irradiation. In this study, 32 one-day-old Swiss albino mouse were used. The subjects in the first group (Group I, n=12) were stained by conventional heating at 37°C for 72 hours, as described by Inouye. The ones in the second group (Group II, n=12) were stained by microwave oven at 40.47±4.8°C for 8 hours. The subjects in the last group (Group Ill, n=12) were stained by conventional heating at 48°C for 8 hours. When all subjects were examined for the quality of the staining and its sufficiency for the detection of any skeletal malformation, no significant difference was found between the Group I and Group II. But Group Ill has been found significantly worse then these two groups. The staining time that originally was 72 in lnouye's method has been reduced to 8 hours by the use of microwave oven. It is concluded that microwave irradiation can be used to decrease the time required for double skeletal staining.
Double skeletal staining methods are used to investigate the probable teratogenic effects of chemical substances on the skeletal system. In these studies, in order to perform appropriate statistical evaluation, the number of subjects should be large enough. Therefore, besides being reliable and permanent, the selected method should also be a short one, which will be completed in a short time. One mostly used double skeletal staining method is the Inouye's method. The aim of this study is to reduce the staining time of the Inouye's method, which originally is 48-72 hours, by the use of microwave irradiation. In this study, 32 one-day-old Swiss albino mouse were used. The subjects in the first group (Group I, n=12) were stained by conventional heating at 37°C for 72 hours, as described by Inouye. The ones in the second group (Group II, n=12) were stained by microwave oven at 40.47±4.8°C for 8 hours. The subjects in the last group (Group Ill, n=12) were stained by conventional heating at 48°C for 8 hours. When all subjects were examined for the quality of the staining and its sufficiency for the detection of any skeletal malformation, no significant difference was found between the Group I and Group II. But Group Ill has been found significantly worse then these two groups. The staining time that originally was 72 in lnouye's method has been reduced to 8 hours by the use of microwave oven. It is concluded that microwave irradiation can be used to decrease the time required for double skeletal staining.
Description
Keywords
Mikrodalga fırın, İskelet boyaması, Microwave oven, Skeletal staining
Citation
Sunay, F. B. (2000). İkili iskelet boyamalarında mikrodalga ışınımının kullanılması. Yayınlanmamış tıpta uzmanlık tezi. Uludağ Üniversitesi Tıp Fakültesi.