Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa

dc.contributor.buuauthorAlçay, Selim
dc.contributor.buuauthorToker, M. Berk
dc.contributor.buuauthorÖnder, N. Tekin
dc.contributor.buuauthorGökçe, Elif
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0002-7678-3289tr_TR
dc.contributor.orcid0000-0001-5141-0008tr_TR
dc.contributor.orcid0000-0003-4033-9749tr_TR
dc.contributor.researcheridABA-6294-2020tr_TR
dc.contributor.scopusid56099810300tr_TR
dc.contributor.scopusid56480349200tr_TR
dc.contributor.scopusid56779799700tr_TR
dc.contributor.scopusid55670728900tr_TR
dc.date.accessioned2022-12-29T07:06:06Z
dc.date.available2022-12-29T07:06:06Z
dc.date.issued2016-11-25
dc.description.abstractThe aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.en_US
dc.identifier.citationAlçay, S. vd. (2017). ''Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa''. Cryobiology, 74, 81-85.en_US
dc.identifier.endpage85tr_TR
dc.identifier.issn0011-2240
dc.identifier.pubmed27908685tr_TR
dc.identifier.scopus2-s2.0-85007492923tr_TR
dc.identifier.startpage81tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2016.11.011
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0011224016301808
dc.identifier.uri1090-2392
dc.identifier.urihttp://hdl.handle.net/11452/30154
dc.identifier.volume74tr_TR
dc.identifier.wos000393533700013tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.journalCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLife sciences & biomedicine - other topicsen_US
dc.subjectPhysiologyen_US
dc.subjectCryopreservationen_US
dc.subjectGoat semenen_US
dc.subjectLIncubation resilienceen_US
dc.subjectRoyal jellyen_US
dc.subjectRam sperm parametersen_US
dc.subjectYolk-based extenderen_US
dc.subjectEgg-yolken_US
dc.subjectSemen frozenen_US
dc.subjectCryopreservationen_US
dc.subjectAntioxidantsen_US
dc.subjectFertilityen_US
dc.subjectPlasmaen_US
dc.subject.emtreeDNAen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreeRoyal jellyen_US
dc.subject.emtreeCryoprotective agenten_US
dc.subject.emtreeFatty aciden_US
dc.subject.emtreeRoyal jellyen_US
dc.subject.emtreeSoybean proteinen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell membraneen_US
dc.subject.emtreeConcentration (parameters)en_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDNA damageen_US
dc.subject.emtreeDNA fragmentationen_US
dc.subject.emtreeFfreeze thawingen_US
dc.subject.emtreeGoaten_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeSemen analysisen_US
dc.subject.emtreeSpermatozoonen_US
dc.subject.emtreeSpermatozoon motilityen_US
dc.subject.emtreeAcrosomeen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeArtificial inseminationen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreeSpermen_US
dc.subject.emtreeSperm preservationen_US
dc.subject.emtreeTUNEL assayen_US
dc.subject.emtreeVeterinaryen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell membraneen_US
dc.subject.emtreeConcentration (parameters)en_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDNA damageen_US
dc.subject.emtreeDNA fragmentationen_US
dc.subject.emtreeFreeze thawingen_US
dc.subject.emtreeGoaten_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeSemen analysisen_US
dc.subject.emtreeSpermatozoonen_US
dc.subject.emtreeSpermatozoon motilityen_US
dc.subject.emtreeAcrosomeen_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeArtificial inseminationen_US
dc.subject.emtreeCryopreservationen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.emtreePhysiologyen_US
dc.subject.emtreeSpermen_US
dc.subject.emtreeSperm preservationen_US
dc.subject.emtreeTUNEL assayen_US
dc.subject.emtreeVeterinaryen_US
dc.subject.meshAcrosomeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell membraneen_US
dc.subject.meshCryopreservationen_US
dc.subject.meshCryoprotective agentsen_US
dc.subject.meshDNA damageen_US
dc.subject.meshFatty acidsen_US
dc.subject.meshGoatsen_US
dc.subject.meshIn situ nick-end labelingen_US
dc.subject.meshInsemination, artificialen_US
dc.subject.meshLecithinsen_US
dc.subject.meshMaleen_US
dc.subject.meshSemenen_US
dc.subject.meshSemen preservationen_US
dc.subject.meshSoybean proteinsen_US
dc.subject.meshSperm motilityen_US
dc.subject.scopusExtender; Semen; Semen Extendersen_US
dc.subject.wosBiologyen_US
dc.subject.wosPhysiologyen_US
dc.titleRoyal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoaen_US
dc.typeArticle
dc.wos.quartileQ2 (Biology)en_US
dc.wos.quartileQ3 (Physiology)en_US

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