Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa
dc.contributor.buuauthor | Alçay, Selim | |
dc.contributor.buuauthor | Toker, M. Berk | |
dc.contributor.buuauthor | Önder, N. Tekin | |
dc.contributor.buuauthor | Gökçe, Elif | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı. | tr_TR |
dc.contributor.orcid | 0000-0002-7678-3289 | tr_TR |
dc.contributor.orcid | 0000-0001-5141-0008 | tr_TR |
dc.contributor.orcid | 0000-0003-4033-9749 | tr_TR |
dc.contributor.researcherid | ABA-6294-2020 | tr_TR |
dc.contributor.scopusid | 56099810300 | tr_TR |
dc.contributor.scopusid | 56480349200 | tr_TR |
dc.contributor.scopusid | 56779799700 | tr_TR |
dc.contributor.scopusid | 55670728900 | tr_TR |
dc.date.accessioned | 2022-12-29T07:06:06Z | |
dc.date.available | 2022-12-29T07:06:06Z | |
dc.date.issued | 2016-11-25 | |
dc.description.abstract | The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation. | en_US |
dc.identifier.citation | Alçay, S. vd. (2017). ''Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa''. Cryobiology, 74, 81-85. | en_US |
dc.identifier.endpage | 85 | tr_TR |
dc.identifier.issn | 0011-2240 | |
dc.identifier.pubmed | 27908685 | tr_TR |
dc.identifier.scopus | 2-s2.0-85007492923 | tr_TR |
dc.identifier.startpage | 81 | tr_TR |
dc.identifier.uri | https://doi.org/10.1016/j.cryobiol.2016.11.011 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0011224016301808 | |
dc.identifier.uri | 1090-2392 | |
dc.identifier.uri | http://hdl.handle.net/11452/30154 | |
dc.identifier.volume | 74 | tr_TR |
dc.identifier.wos | 000393533700013 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.journal | Cryobiology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Life sciences & biomedicine - other topics | en_US |
dc.subject | Physiology | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | Goat semen | en_US |
dc.subject | LIncubation resilience | en_US |
dc.subject | Royal jelly | en_US |
dc.subject | Ram sperm parameters | en_US |
dc.subject | Yolk-based extender | en_US |
dc.subject | Egg-yolk | en_US |
dc.subject | Semen frozen | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | Antioxidants | en_US |
dc.subject | Fertility | en_US |
dc.subject | Plasma | en_US |
dc.subject.emtree | DNA | en_US |
dc.subject.emtree | Phosphatidylcholine | en_US |
dc.subject.emtree | Royal jelly | en_US |
dc.subject.emtree | Cryoprotective agent | en_US |
dc.subject.emtree | Fatty acid | en_US |
dc.subject.emtree | Royal jelly | en_US |
dc.subject.emtree | Soybean protein | en_US |
dc.subject.emtree | Phosphatidylcholine | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Cell membrane | en_US |
dc.subject.emtree | Concentration (parameters) | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Cryopreservation | en_US |
dc.subject.emtree | DNA damage | en_US |
dc.subject.emtree | DNA fragmentation | en_US |
dc.subject.emtree | Ffreeze thawing | en_US |
dc.subject.emtree | Goat | en_US |
dc.subject.emtree | Male | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | Semen analysis | en_US |
dc.subject.emtree | Spermatozoon | en_US |
dc.subject.emtree | Spermatozoon motility | en_US |
dc.subject.emtree | Acrosome | en_US |
dc.subject.emtree | Animal | en_US |
dc.subject.emtree | Artificial insemination | en_US |
dc.subject.emtree | Cryopreservation | en_US |
dc.subject.emtree | Drug effects | en_US |
dc.subject.emtree | Physiology | en_US |
dc.subject.emtree | Sperm | en_US |
dc.subject.emtree | Sperm preservation | en_US |
dc.subject.emtree | TUNEL assay | en_US |
dc.subject.emtree | Veterinary | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Cell membrane | en_US |
dc.subject.emtree | Concentration (parameters) | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Cryopreservation | en_US |
dc.subject.emtree | DNA damage | en_US |
dc.subject.emtree | DNA fragmentation | en_US |
dc.subject.emtree | Freeze thawing | en_US |
dc.subject.emtree | Goat | en_US |
dc.subject.emtree | Male | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | Semen analysis | en_US |
dc.subject.emtree | Spermatozoon | en_US |
dc.subject.emtree | Spermatozoon motility | en_US |
dc.subject.emtree | Acrosome | en_US |
dc.subject.emtree | Animal | en_US |
dc.subject.emtree | Artificial insemination | en_US |
dc.subject.emtree | Cryopreservation | en_US |
dc.subject.emtree | Drug effects | en_US |
dc.subject.emtree | Physiology | en_US |
dc.subject.emtree | Sperm | en_US |
dc.subject.emtree | Sperm preservation | en_US |
dc.subject.emtree | TUNEL assay | en_US |
dc.subject.emtree | Veterinary | en_US |
dc.subject.mesh | Acrosome | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell membrane | en_US |
dc.subject.mesh | Cryopreservation | en_US |
dc.subject.mesh | Cryoprotective agents | en_US |
dc.subject.mesh | DNA damage | en_US |
dc.subject.mesh | Fatty acids | en_US |
dc.subject.mesh | Goats | en_US |
dc.subject.mesh | In situ nick-end labeling | en_US |
dc.subject.mesh | Insemination, artificial | en_US |
dc.subject.mesh | Lecithins | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Semen | en_US |
dc.subject.mesh | Semen preservation | en_US |
dc.subject.mesh | Soybean proteins | en_US |
dc.subject.mesh | Sperm motility | en_US |
dc.subject.scopus | Extender; Semen; Semen Extenders | en_US |
dc.subject.wos | Biology | en_US |
dc.subject.wos | Physiology | en_US |
dc.title | Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa | en_US |
dc.type | Article | |
dc.wos.quartile | Q2 (Biology) | en_US |
dc.wos.quartile | Q3 (Physiology) | en_US |
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