Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase

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dc.contributor.buuauthorKök, Senay
dc.contributor.buuauthorOsman, Bilgen
dc.contributor.buuauthorKara, Ali
dc.contributor.buuauthorBeşirli, Necati
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.tr_TR
dc.contributor.researcheridAAG-6271-2019tr_TR
dc.contributor.researcheridABF-4791-2020tr_TR
dc.contributor.scopusid37054138900tr_TR
dc.contributor.scopusid15221651200tr_TR
dc.contributor.scopusid7102824859tr_TR
dc.contributor.scopusid37015388100tr_TR
dc.date.accessioned2022-01-04T07:15:37Z
dc.date.available2022-01-04T07:15:37Z
dc.date.issued2011-06-05
dc.description.abstractPoly(ethylene glycol dimethacrylate-1-vinyl-1,2,4-triazole) [poly(EGDMA-VTAZ)] beads with an average diameter of 100-200 μm were obtained by the copolymerization of ethylene glycol dimethacrylate (EGDMA) with 1-vinyl-1,2,4-triazole (VTAZ). The copolymer hydrogel bead composition was determined by elemental analysis and was found to contain 5 EGDMA monomer units for each VTAZ monomer unit. The poly(EGDMA-VTAZ) beads were characterized by swelling studies and scanning electron microscopy (SEM). The specific surface area of the poly(EGDMA-VTAZ) beads was found 65.8 m2/g. Cu 2+ ions were chelated on the poly(EGDMA-VTAZ) beads. The Cu 2+ loading was 82.6 μmol/g of support. Cu2+-chelated poly(EGDMA-VTAZ) beads with a swelling ratio of 84% were used in the immobilization of Aspergillus niger glucoamylase in a batch system. The maximum glucoamylase adsorption capacity of the poly(EGDMA-VTAZ)-Cu2+ beads was 104 mg/g at pH 6.5. The adsorption isotherm of the poly(EGDMA-VTAZ)-Cu 2+ beads fitted well with the Langmuir model. Adsorption kinetics data were tested with pseudo-first- and second-order models. The kinetic studies showed that the adsorption followed a pseudo-second-order reaction model. The Michaelis constant value for the immobilized glucoamylase (1.15 mg/mL) was higher than that for free glucoamylase (1.00 mg/mL). The maximum initial rate of the reaction values were 42.9 U/mg for the free enzyme and 33.3 U/mg for the immobilized enzyme. The optimum temperature for the immobilized preparation of poly(EGDMA-VTAZ)-Cu2+-glucoamylase was 65°C; this was 5°C higher than that of the free enzyme at 60°C. The glucoamylase adsorption capacity and adsorbed enzyme activity slightly decreased after 10 batch successive reactions; this demonstrated the usefulness of the enzyme-loaded beads in biocatalytic applications. The storage stability was found to increase with immobilization.en_US
dc.identifier.citationKök, S. vd. (2011). "Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase". Journal of Applied Polymer Science, 120(5), 2563-2570.en_US
dc.identifier.endpage2570tr_TR
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue5tr_TR
dc.identifier.scopus2-s2.0-79952483004tr_TR
dc.identifier.startpage2563tr_TR
dc.identifier.urihttps://doi.org/10.1002/app.33325
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/app.33325
dc.identifier.urihttp://hdl.handle.net/11452/23834
dc.identifier.volume120tr_TR
dc.identifier.wos000288340100008tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.bapF-2008/55tr_TR
dc.relation.journalJournal of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolymer scienceen_US
dc.subjectAdsorptionen_US
dc.subjectEenzymesen_US
dc.subjectMetal-polymer complexesen_US
dc.subjectAfiinity chromatographyen_US
dc.subjectCatalaseen_US
dc.subjectAdsorptionen_US
dc.subjectPurificationen_US
dc.subjectAdsorptionen_US
dc.subjectCatalystsen_US
dc.subjectChelationen_US
dc.subjectChemical analysisen_US
dc.subjectCopolymerizationen_US
dc.subjectCopolymersen_US
dc.subjectEnzyme activityen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectEthyleneen_US
dc.subjectEthylene glycolen_US
dc.subjectMonomersen_US
dc.subjectNitrogen compoundsen_US
dc.subjectScanning electron microscopyen_US
dc.subjectAdsorbed enzymeen_US
dc.subjectAdsorption capacitiesen_US
dc.subjectAdsorption kineticsen_US
dc.subjectAspergillus nigeren_US
dc.subjectAverage diameteren_US
dc.subjectBatch systemsen_US
dc.subjectBiocatalytic applicationsen_US
dc.subjectCopolymer hydrogelsen_US
dc.subjectElemental analysisen_US
dc.subjectEthylene glycol dimethacrylateen_US
dc.subjectFree enzymeen_US
dc.subjectGlucoamylaseen_US
dc.subjectImmobilized enzymeen_US
dc.subjectInitial rateen_US
dc.subjectKinetic studyen_US
dc.subjectLangmuir modelsen_US
dc.subjectMetal-polymer complexesen_US
dc.subjectMichaelis constantsen_US
dc.subjectMonomer unitsen_US
dc.subjectOptimum temperatureen_US
dc.subjectReversible immobilizationen_US
dc.subjectSecond-order modelsen_US
dc.subjectSecond-order reactionen_US
dc.subjectSEMen_US
dc.subjectStorage stabilityen_US
dc.subjectSwelling ratioen_US
dc.subjectSwelling studiesen_US
dc.subjectEnzymesen_US
dc.subject.scopusCibacron Blue F 3Ga; Cryogels; Muramidaseen_US
dc.subject.wosPolymer scienceen_US
dc.titleVinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylaseen_US
dc.typeArticle
dc.wos.quartileQ3en_US

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