Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase
dc.contributor.buuauthor | Kök, Senay | |
dc.contributor.buuauthor | Osman, Bilgen | |
dc.contributor.buuauthor | Kara, Ali | |
dc.contributor.buuauthor | Beşirli, Necati | |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı. | tr_TR |
dc.contributor.researcherid | AAG-6271-2019 | tr_TR |
dc.contributor.researcherid | ABF-4791-2020 | tr_TR |
dc.contributor.scopusid | 37054138900 | tr_TR |
dc.contributor.scopusid | 15221651200 | tr_TR |
dc.contributor.scopusid | 7102824859 | tr_TR |
dc.contributor.scopusid | 37015388100 | tr_TR |
dc.date.accessioned | 2022-01-04T07:15:37Z | |
dc.date.available | 2022-01-04T07:15:37Z | |
dc.date.issued | 2011-06-05 | |
dc.description.abstract | Poly(ethylene glycol dimethacrylate-1-vinyl-1,2,4-triazole) [poly(EGDMA-VTAZ)] beads with an average diameter of 100-200 μm were obtained by the copolymerization of ethylene glycol dimethacrylate (EGDMA) with 1-vinyl-1,2,4-triazole (VTAZ). The copolymer hydrogel bead composition was determined by elemental analysis and was found to contain 5 EGDMA monomer units for each VTAZ monomer unit. The poly(EGDMA-VTAZ) beads were characterized by swelling studies and scanning electron microscopy (SEM). The specific surface area of the poly(EGDMA-VTAZ) beads was found 65.8 m2/g. Cu 2+ ions were chelated on the poly(EGDMA-VTAZ) beads. The Cu 2+ loading was 82.6 μmol/g of support. Cu2+-chelated poly(EGDMA-VTAZ) beads with a swelling ratio of 84% were used in the immobilization of Aspergillus niger glucoamylase in a batch system. The maximum glucoamylase adsorption capacity of the poly(EGDMA-VTAZ)-Cu2+ beads was 104 mg/g at pH 6.5. The adsorption isotherm of the poly(EGDMA-VTAZ)-Cu 2+ beads fitted well with the Langmuir model. Adsorption kinetics data were tested with pseudo-first- and second-order models. The kinetic studies showed that the adsorption followed a pseudo-second-order reaction model. The Michaelis constant value for the immobilized glucoamylase (1.15 mg/mL) was higher than that for free glucoamylase (1.00 mg/mL). The maximum initial rate of the reaction values were 42.9 U/mg for the free enzyme and 33.3 U/mg for the immobilized enzyme. The optimum temperature for the immobilized preparation of poly(EGDMA-VTAZ)-Cu2+-glucoamylase was 65°C; this was 5°C higher than that of the free enzyme at 60°C. The glucoamylase adsorption capacity and adsorbed enzyme activity slightly decreased after 10 batch successive reactions; this demonstrated the usefulness of the enzyme-loaded beads in biocatalytic applications. The storage stability was found to increase with immobilization. | en_US |
dc.identifier.citation | Kök, S. vd. (2011). "Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase". Journal of Applied Polymer Science, 120(5), 2563-2570. | en_US |
dc.identifier.endpage | 2570 | tr_TR |
dc.identifier.issn | 0021-8995 | |
dc.identifier.issn | 1097-4628 | |
dc.identifier.issue | 5 | tr_TR |
dc.identifier.scopus | 2-s2.0-79952483004 | tr_TR |
dc.identifier.startpage | 2563 | tr_TR |
dc.identifier.uri | https://doi.org/10.1002/app.33325 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1002/app.33325 | |
dc.identifier.uri | http://hdl.handle.net/11452/23834 | |
dc.identifier.volume | 120 | tr_TR |
dc.identifier.wos | 000288340100008 | tr_TR |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.bap | F-2008/55 | tr_TR |
dc.relation.journal | Journal of Applied Polymer Science | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Polymer science | en_US |
dc.subject | Adsorption | en_US |
dc.subject | Eenzymes | en_US |
dc.subject | Metal-polymer complexes | en_US |
dc.subject | Afiinity chromatography | en_US |
dc.subject | Catalase | en_US |
dc.subject | Adsorption | en_US |
dc.subject | Purification | en_US |
dc.subject | Adsorption | en_US |
dc.subject | Catalysts | en_US |
dc.subject | Chelation | en_US |
dc.subject | Chemical analysis | en_US |
dc.subject | Copolymerization | en_US |
dc.subject | Copolymers | en_US |
dc.subject | Enzyme activity | en_US |
dc.subject | Enzyme immobilization | en_US |
dc.subject | Ethylene | en_US |
dc.subject | Ethylene glycol | en_US |
dc.subject | Monomers | en_US |
dc.subject | Nitrogen compounds | en_US |
dc.subject | Scanning electron microscopy | en_US |
dc.subject | Adsorbed enzyme | en_US |
dc.subject | Adsorption capacities | en_US |
dc.subject | Adsorption kinetics | en_US |
dc.subject | Aspergillus niger | en_US |
dc.subject | Average diameter | en_US |
dc.subject | Batch systems | en_US |
dc.subject | Biocatalytic applications | en_US |
dc.subject | Copolymer hydrogels | en_US |
dc.subject | Elemental analysis | en_US |
dc.subject | Ethylene glycol dimethacrylate | en_US |
dc.subject | Free enzyme | en_US |
dc.subject | Glucoamylase | en_US |
dc.subject | Immobilized enzyme | en_US |
dc.subject | Initial rate | en_US |
dc.subject | Kinetic study | en_US |
dc.subject | Langmuir models | en_US |
dc.subject | Metal-polymer complexes | en_US |
dc.subject | Michaelis constants | en_US |
dc.subject | Monomer units | en_US |
dc.subject | Optimum temperature | en_US |
dc.subject | Reversible immobilization | en_US |
dc.subject | Second-order models | en_US |
dc.subject | Second-order reaction | en_US |
dc.subject | SEM | en_US |
dc.subject | Storage stability | en_US |
dc.subject | Swelling ratio | en_US |
dc.subject | Swelling studies | en_US |
dc.subject | Enzymes | en_US |
dc.subject.scopus | Cibacron Blue F 3Ga; Cryogels; Muramidase | en_US |
dc.subject.wos | Polymer science | en_US |
dc.title | Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase | en_US |
dc.type | Article | |
dc.wos.quartile | Q3 | en_US |
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