A capillary polymerase chain reaction for Salmonella detection from poultry meat

dc.contributor.buuauthorGünaydın, Elçin
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Besin Hijyeni ve Teknolojisi Anabilim Dalı.tr_TR
dc.contributor.researcheridE-3867-2010tr_TR
dc.contributor.researcheridAAI-1101-2021tr_TR
dc.contributor.scopusid8350555600tr_TR
dc.contributor.scopusid6602558950tr_TR
dc.contributor.scopusid6601971539tr_TR
dc.date.accessioned2023-03-13T05:40:49Z
dc.date.available2023-03-13T05:40:49Z
dc.date.issued2007-01
dc.description.abstractAims: In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. Methods and Results: Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite cystine broth (SCB) artificially contaminated with 10-fold dilutions of 6 x 10(8) CFU ml(-1) of pure Salmonella enterica ssp. enterica serovar Enteritidis 64K stock culture. Detection limits of cPCR from TTB, RVB and SCB were found as 6, 6 x 10(1) and 6 x 10(4) CFU ml(-1), respectively. In addition, detection limits of bacteriology were also determined as 6 CFU ml(-1) with TTB and SCB, and 6 x 10(1) CFU ml(-1) with RVB. A total of 200 samples, consisting of 100 chicken and 100 turkey meat samples, were tested with optimized cPCR and bacteriology. Eight and six per cent of the chicken meat samples were found to harbour Salmonella by cPCR and standard bacteriology, respectively. Of six Salmonella isolates, four belonged to serogroup D, two to serogroup B. Conclusions: The TTB cultures of both artificially and naturally contaminated samples were found to be superior to those of RVB and SCB cultures in their cPCR results. This cPCR, utilizing template from 18-h TTB primary enrichment broth culture, takes approximately 40 min in the successful detection of Salmonella from poultry meat. Significance and Impact of the study: This study shows that cPCR from TTB enrichment culture of poultry meat would enable rapid detection of Salmonella in laboratories with low sample throughput and limited budget.en_US
dc.identifier.citationGünaydın, E. vd. (2007). "A capillary polymerase chain reaction for Salmonella detection from poultry meat". Letters in Applied Microbiology, 44(1), 24-29.en_US
dc.identifier.endpage29tr_TR
dc.identifier.issn1472-765X
dc.identifier.issn0266-8254
dc.identifier.issue1tr_TR
dc.identifier.pubmed17209810tr_TR
dc.identifier.scopus2-s2.0-33845769884tr_TR
dc.identifier.startpage24tr_TR
dc.identifier.urihttps://doi.org/10.1111/j.1472-765X.2006.02033.x
dc.identifier.urihttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2006.02033.x
dc.identifier.urihttp://hdl.handle.net/11452/31515
dc.identifier.volume44tr_TR
dc.identifier.wos000242964500005tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.journalLetterrs in Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCapillary polymerase chain reactionen_US
dc.subjectPoultry meaten_US
dc.subjectSalmonellaen_US
dc.subjectBacteriologyen_US
dc.subjectCapillary flowen_US
dc.subjectBacteriumen_US
dc.subjectRapid detectionen_US
dc.subjectBiotechnology & applied microbiologyen_US
dc.subjectSalmonella entericaen_US
dc.subjectCell cultureen_US
dc.subjectContaminationen_US
dc.subjectFungien_US
dc.subjectMeatsen_US
dc.subjectOptimizationen_US
dc.subjectDetection methoden_US
dc.subjectDNAen_US
dc.subjectMeaten_US
dc.subjectPolymerase chain reactionen_US
dc.subjectPoultryen_US
dc.subjectMicrobiologyen_US
dc.subjectMultiplex pcren_US
dc.subjectChickenen_US
dc.subjectCultureen_US
dc.subjectSpp.en_US
dc.subjectIdentificationen_US
dc.subjectAmplificationen_US
dc.subjectSamplesen_US
dc.subjectGeneen_US
dc.subjectTyphimuriumen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBacterium detectionen_US
dc.subject.emtreePolymerase chain reactionen_US
dc.subject.emtreeBacterium isolateen_US
dc.subject.emtreeBlood group typingen_US
dc.subject.emtreeDilutionen_US
dc.subject.emtreeSalmonella entericaen_US
dc.subject.emtreeChickenen_US
dc.subject.emtreeColony forming uniten_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDNA extractionen_US
dc.subject.emtreeDNA templateen_US
dc.subject.emtreeFood contaminationen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePoultryen_US
dc.subject.emtreeSalmonellaen_US
dc.subject.emtreeSerotypeen_US
dc.subject.emtreeTurkey (bird)en_US
dc.subject.meshBacteriological techniquesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDNA, bacterialen_US
dc.subject.meshElectrophoresis, capillaryen_US
dc.subject.meshFood microbiologyen_US
dc.subject.meshPoultry diseasesen_US
dc.subject.meshPoultry productsen_US
dc.subject.meshPolymerase chain reactionen_US
dc.subject.meshSalmonellaen_US
dc.subject.meshSalmonella infections, animalen_US
dc.subject.scopusSalmonella; Food Pathogens; Salmonella Enterica Serovar Enteritidisen_US
dc.subject.wosBiotechnology & applied microbiologyen_US
dc.subject.wosMicrobiologyen_US
dc.titleA capillary polymerase chain reaction for Salmonella detection from poultry meaten_US
dc.typeArticle
dc.wos.quartileQ3en_US

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