Hemorrhage activates proopiomelanocortin neurons in the rat hypothalamus

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dc.contributor.authorLevendusky, M. C.
dc.contributor.authorResch, Garth. E.
dc.contributor.authorVeno, Patricia A.
dc.contributor.authorMillington, W. R.
dc.contributor.buuauthorGöktalay, Gökhan
dc.contributor.buuauthorÇavun, Sinan
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Klinik Farmakoloji Anabilim Dalı.tr_TR
dc.contributor.researcheridAAH-1448-2021tr_TR
dc.contributor.researcheridAAC-9702-2019tr_TR
dc.contributor.scopusid6508023759tr_TR
dc.contributor.scopusid6507468595tr_TR
dc.date.accessioned2021-12-02T05:52:28Z
dc.date.available2021-12-02T05:52:28Z
dc.date.issued2006-01-27
dc.description.abstractSevere blood loss lowers arterial pressure through a central mechanism that is thought to include opioid neurons. In this study, we investigated whether hemorrhage activates proopiomelanocortin (POMC) neurons by measuring Fos immunoreactivity and POMC mRNA levels in the medial basal hypothalamus. Hemorrhage (2.2 ml/100 g body weight over 20 min) increased the number of Fos immunoreactive neurons throughout the rostral-caudal extent of the arcuate nucleus, the retrochiasmatic area and the peri-arcuate region lateral to the arcuate nucleus where POMC neurons are located. Double label immunohistochemistry revealed that hemorrhage increased Fos expression by beta-endorphin immunoreactive neurons significantly. The proportion of beta-endorphin immunoreactive neurons that expressed Fos immunoreactivity increased approximately four-fold, from 11.7 +/- 1.4% in sham-operated control animals to 42.0 +/- 5.2% in hemorrhaged animals. Hemorrhage also increased POMC mRNA levels in the medial basal hypothalamus significantly, consistent with the hypothesis that blood loss activates POMC neurons. To test whether activation of arcuate neurons contributes to the fall in arterial pressure evoked by hemorrhage, we inhibited neuronal activity in the caudal arcuate nucleus by microinjecting the local anesthetic lidocaine (2%; 0.1 or 0.3 mu l) bilaterally 2 min before hemorrhage was initiated. Lidocaine injection inhibited hemorrhagic hypotension and bradycardia significantly although it did not influence arterial pressure or heart rate in non-hemorrhaged rats. These results demonstrate that hemorrhage activates POMC neurons and provide evidence that activation of neurons in the arcuate nucleus plays an important role in the hemodynamic response to hemorrhage.en_US
dc.identifier.citationGöktalay, G. vd. (2006). ''Hemorrhage activates proopiomelanocortin neurons in the rat hypothalamus''. Brain Research, 1070(1), 45-55.en_US
dc.identifier.endpage55tr_TR
dc.identifier.issn0006-8993
dc.identifier.issn1872-6240
dc.identifier.issue1tr_TR
dc.identifier.pubmed16403465tr_TR
dc.identifier.scopus2-s2.0-32644474399tr_TR
dc.identifier.startpage45tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.brainres.2005.11.076
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0006899305015866
dc.identifier.urihttp://hdl.handle.net/11452/22939
dc.identifier.volume1070tr_TR
dc.identifier.wos000236099000006tr_TR
dc.indexed.pubmedPubmeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalBrain Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNeurosciences & neurologyen_US
dc.subjectProopiomelanocortinen_US
dc.subjectHemorrhageen_US
dc.subjectBlood pressurseen_US
dc.subjectArcuate nucleusen_US
dc.subjectβ-Endorphinen_US
dc.subjectAnimaliaen_US
dc.subjectc-Fosen_US
dc.subjectAcute hypovolemiaen_US
dc.subjectOpiate antagonistsen_US
dc.subjectDecompensatory phaseen_US
dc.subjectPeriaqueductal grayen_US
dc.subjectVentrolateral medullaen_US
dc.subjectParaventricular nucleusen_US
dc.subjectBeta-endorphinen_US
dc.subjectHemodynamic-responseen_US
dc.subjectFos expressionen_US
dc.subjectOpioid receptorsen_US
dc.subject.emtreeProtein fosen_US
dc.subject.emtreeProopiomelanocortinen_US
dc.subject.emtreeLidocaineen_US
dc.subject.emtreeBeta endorphinen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreestatistical significanceen_US
dc.subject.emtreeRaten_US
dc.subject.emtreeAnimal tissueen_US
dc.subject.emtreeArcuate nucleusen_US
dc.subject.emtreeArterial pressureen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBleedingen_US
dc.subject.emtreeBradycardiaen_US
dc.subject.emtreeCell activationen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeHeart rateen_US
dc.subject.emtreeHemodynamicsen_US
dc.subject.emtreeHypotensionen_US
dc.subject.emtreeHypothalamusen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeImmunoreactivityen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNerve cellen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeNonhumanen_US
dc.subject.meshRNA, messengeren_US
dc.subject.meshRats, sprague-dawleyen_US
dc.subject.meshRatsen_US
dc.subject.meshProto-oncogene proteins c-fosen_US
dc.subject.meshPro-opiomelanocortinen_US
dc.subject.meshNeuronsen_US
dc.subject.meshMaleen_US
dc.subject.meshLidocaineen_US
dc.subject.meshInjectionsen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshHypothalamus, middleen_US
dc.subject.meshHypothalamusen_US
dc.subject.meshHypotensionen_US
dc.subject.meshHemorrhageen_US
dc.subject.meshBradycardiaen_US
dc.subject.meshBeta-endorphinen_US
dc.subject.meshArcuate nucleusen_US
dc.subject.meshAnimalsen_US
dc.subject.scopusVasovagal Syncope; Thermodilution; Hemorrhagesen_US
dc.subject.wosNeurosciencesen_US
dc.titleHemorrhage activates proopiomelanocortin neurons in the rat hypothalamusen_US
dc.typeArticle
dc.wos.quartileQ3en_US

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