Publication:
Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis

dc.contributor.authorOkuturlar, Yıldız
dc.contributor.authorKeskin, Kürşad
dc.contributor.authorBurgazlıoğlu, Başak
dc.contributor.buuauthorBölük, Gülçin
dc.contributor.buuauthorKazak, Esra
dc.contributor.buuauthorÖzkalemkaş, Fahir
dc.contributor.buuauthorEner, Beyza
dc.contributor.buuauthorAkalın, Halis
dc.contributor.buuauthorAğca, Harun
dc.contributor.buuauthorAli, Rıdvan
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentİç Hastalıkları Ana Bilim Dalı
dc.contributor.departmentHematoloji Ünitesi
dc.contributor.orcid0000-0002-4803-8206tr_TR
dc.contributor.orcid0000-0002-2651-2034tr_TR
dc.contributor.researcheridAAG-8523-2021tr_TR
dc.contributor.researcheridAAG-8495-2021tr_TR
dc.contributor.researcheridAAG-8459-2021tr_TR
dc.contributor.researcheridAAU-8952-2020tr_TR
dc.contributor.researcheridAAH-4027-2021tr_TR
dc.contributor.scopusid56196200800tr_TR
dc.contributor.scopusid24921238200tr_TR
dc.contributor.scopusid6601912387tr_TR
dc.contributor.scopusid15053025300tr_TR
dc.contributor.scopusid57207553671tr_TR
dc.contributor.scopusid15759379900tr_TR
dc.contributor.scopusid7201813027tr_TR
dc.date.accessioned2022-12-12T08:03:54Z
dc.date.available2022-12-12T08:03:54Z
dc.date.issued2015-06-28
dc.description.abstractBckground/aim: Invasive aspergillosis (IA) is a fatal infection that is difficult to diagnose in immunocompromised patients. In this study, Aspergillus-specific DNA was searched using real-time PCR (RT-PCR) in serum samples. Galactomannan (GM) and/or beta-D-glucan (BDG) tests were previously performed on these samples for 70 neutropenic patients with hematological malignancy. Materials and methods: The patients were categorized according to the criteria of the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG). Among the patient serum samples, the first positive GM or BDG test sample and the median sample of GM or BDG test for negative patients were used to detect DNA levels by RT-PCR method (Light Cycler 480, Roche Molecular Biochemicals, Meylan, France) using a commercial kit (Way2Gene Fungi; Genmar, Izmir, Turkey). Results: When the proven and probable IA group were considered as real patients, sensitivity of Aspergillus-specific DNA test was 90%, specificity was 73.3%, positive predictive value was 81.8%, and negative predictive value was 84.6%. Conclusion: This study found that searching for specific DNA by RT-PCR method has a sensitivity as high as the GM test. Although specificity was rather low, it was concluded that it can be used jointly with GM and BDG tests after decreasing contamination by severe laboratory applications.en_US
dc.identifier.citationBölük, G. vd. (2016). "Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis". Turkish Journal of Medical Sciences, 46(2), 335-342.tr_TR
dc.identifier.endpage342tr_TR
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue2tr_TR
dc.identifier.pubmed27511494tr_TR
dc.identifier.scopus2-s2.0-84958525768tr_TR
dc.identifier.startpage335tr_TR
dc.identifier.urihttps://doi.org/10.3906/sag-1408-100
dc.identifier.urihttps://journals.tubitak.gov.tr/medical/vol46/iss2/15/
dc.identifier.urihttp://hdl.handle.net/11452/29803
dc.identifier.volume46tr_TR
dc.identifier.wos000370271100015
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.trdizinTrDizintr_TR
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherTÜBİTAKtr_TR
dc.relation.bapKUAP(T) 2012/28tr_TR
dc.relation.collaborationSanayitr_TR
dc.relation.journalTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGeneral & internal medicineen_US
dc.subjectInvasive aspergillosisen_US
dc.subjectGalactomannan testen_US
dc.subjectBeta-D-glucan testen_US
dc.subjectSpecific DNAen_US
dc.subjectLinked-immunosorbent-assayen_US
dc.subjectReal-time PCRen_US
dc.subjectPolymerase-chain-reactionen_US
dc.subjectCell transplant recipientsen_US
dc.subjectFungal-infectionsen_US
dc.subjectCirculating galactomannanen_US
dc.subjectPulmonary aspergillosisen_US
dc.subjectBronchoalveolar lavageen_US
dc.subjectClinical-evaluationen_US
dc.subjectAntigen-detectionen_US
dc.subject.emtreeAspergillus DNAen_US
dc.subject.emtreeBeta d glucanen_US
dc.subject.emtreeBeta glucanen_US
dc.subject.emtreeFungal DNAen_US
dc.subject.emtreeGalactomannanen_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeDNAen_US
dc.subject.emtreeGalactomannanen_US
dc.subject.emtreeMannanen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeComparative studyen_US
dc.subject.emtreeDNA extractionen_US
dc.subject.emtreeHematologic malignancyen_US
dc.subject.emtreeHigh risk patienten_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman tissueen_US
dc.subject.emtreeInvasive aspergillosisen_US
dc.subject.emtreeMajor clinical studyen_US
dc.subject.emtreeMicroscopyen_US
dc.subject.emtreePredictive valueen_US
dc.subject.emtreeReal time polymerase chain reactionen_US
dc.subject.emtreeSensitivity and specificityen_US
dc.subject.emtreeAspergillosisen_US
dc.subject.emtreeAspergillusen_US
dc.subject.emtreeHematologic diseaseen_US
dc.subject.emtreeTurkey (bird)en_US
dc.subject.meshAdulten_US
dc.subject.meshAspergillosisen_US
dc.subject.meshAspergillusen_US
dc.subject.meshDNAen_US
dc.subject.meshHematologic neoplasmsen_US
dc.subject.meshHumansen_US
dc.subject.meshMannansen_US
dc.subject.meshTurkeyen_US
dc.subject.scopusInvasive Aspergillosis; Mycoses; Systemic Mycosisen_US
dc.subject.wosMedicine, general & internalen_US
dc.titleComparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosisen_US
dc.typeArticle
dc.wos.quartileQ3en_US
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalıtr_TR
local.contributor.departmentTıp Fakültesi/İç Hastalıkları Ana Bilim Dalı/Hematoloji Ünitesitr_TR
local.contributor.departmentTıp Fakültesi/Tıbbi Mikrobiyoloji Ana Bilim Dalıtr_TR

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