Publication: Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis
dc.contributor.author | Okuturlar, Yıldız | |
dc.contributor.author | Keskin, Kürşad | |
dc.contributor.author | Burgazlıoğlu, Başak | |
dc.contributor.buuauthor | Bölük, Gülçin | |
dc.contributor.buuauthor | Kazak, Esra | |
dc.contributor.buuauthor | Özkalemkaş, Fahir | |
dc.contributor.buuauthor | Ener, Beyza | |
dc.contributor.buuauthor | Akalın, Halis | |
dc.contributor.buuauthor | Ağca, Harun | |
dc.contributor.buuauthor | Ali, Rıdvan | |
dc.contributor.department | Tıp Fakültesi | |
dc.contributor.department | İç Hastalıkları Ana Bilim Dalı | |
dc.contributor.department | Hematoloji Ünitesi | |
dc.contributor.orcid | 0000-0002-4803-8206 | tr_TR |
dc.contributor.orcid | 0000-0002-2651-2034 | tr_TR |
dc.contributor.researcherid | AAG-8523-2021 | tr_TR |
dc.contributor.researcherid | AAG-8495-2021 | tr_TR |
dc.contributor.researcherid | AAG-8459-2021 | tr_TR |
dc.contributor.researcherid | AAU-8952-2020 | tr_TR |
dc.contributor.researcherid | AAH-4027-2021 | tr_TR |
dc.contributor.scopusid | 56196200800 | tr_TR |
dc.contributor.scopusid | 24921238200 | tr_TR |
dc.contributor.scopusid | 6601912387 | tr_TR |
dc.contributor.scopusid | 15053025300 | tr_TR |
dc.contributor.scopusid | 57207553671 | tr_TR |
dc.contributor.scopusid | 15759379900 | tr_TR |
dc.contributor.scopusid | 7201813027 | tr_TR |
dc.date.accessioned | 2022-12-12T08:03:54Z | |
dc.date.available | 2022-12-12T08:03:54Z | |
dc.date.issued | 2015-06-28 | |
dc.description.abstract | Bckground/aim: Invasive aspergillosis (IA) is a fatal infection that is difficult to diagnose in immunocompromised patients. In this study, Aspergillus-specific DNA was searched using real-time PCR (RT-PCR) in serum samples. Galactomannan (GM) and/or beta-D-glucan (BDG) tests were previously performed on these samples for 70 neutropenic patients with hematological malignancy. Materials and methods: The patients were categorized according to the criteria of the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG). Among the patient serum samples, the first positive GM or BDG test sample and the median sample of GM or BDG test for negative patients were used to detect DNA levels by RT-PCR method (Light Cycler 480, Roche Molecular Biochemicals, Meylan, France) using a commercial kit (Way2Gene Fungi; Genmar, Izmir, Turkey). Results: When the proven and probable IA group were considered as real patients, sensitivity of Aspergillus-specific DNA test was 90%, specificity was 73.3%, positive predictive value was 81.8%, and negative predictive value was 84.6%. Conclusion: This study found that searching for specific DNA by RT-PCR method has a sensitivity as high as the GM test. Although specificity was rather low, it was concluded that it can be used jointly with GM and BDG tests after decreasing contamination by severe laboratory applications. | en_US |
dc.identifier.citation | Bölük, G. vd. (2016). "Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis". Turkish Journal of Medical Sciences, 46(2), 335-342. | tr_TR |
dc.identifier.endpage | 342 | tr_TR |
dc.identifier.issn | 1300-0144 | |
dc.identifier.issn | 1303-6165 | |
dc.identifier.issue | 2 | tr_TR |
dc.identifier.pubmed | 27511494 | tr_TR |
dc.identifier.scopus | 2-s2.0-84958525768 | tr_TR |
dc.identifier.startpage | 335 | tr_TR |
dc.identifier.uri | https://doi.org/10.3906/sag-1408-100 | |
dc.identifier.uri | https://journals.tubitak.gov.tr/medical/vol46/iss2/15/ | |
dc.identifier.uri | http://hdl.handle.net/11452/29803 | |
dc.identifier.volume | 46 | tr_TR |
dc.identifier.wos | 000370271100015 | |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.trdizin | TrDizin | tr_TR |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | TÜBİTAK | tr_TR |
dc.relation.bap | KUAP(T) 2012/28 | tr_TR |
dc.relation.collaboration | Sanayi | tr_TR |
dc.relation.journal | Turkish Journal of Medical Sciences | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | General & internal medicine | en_US |
dc.subject | Invasive aspergillosis | en_US |
dc.subject | Galactomannan test | en_US |
dc.subject | Beta-D-glucan test | en_US |
dc.subject | Specific DNA | en_US |
dc.subject | Linked-immunosorbent-assay | en_US |
dc.subject | Real-time PCR | en_US |
dc.subject | Polymerase-chain-reaction | en_US |
dc.subject | Cell transplant recipients | en_US |
dc.subject | Fungal-infections | en_US |
dc.subject | Circulating galactomannan | en_US |
dc.subject | Pulmonary aspergillosis | en_US |
dc.subject | Bronchoalveolar lavage | en_US |
dc.subject | Clinical-evaluation | en_US |
dc.subject | Antigen-detection | en_US |
dc.subject.emtree | Aspergillus DNA | en_US |
dc.subject.emtree | Beta d glucan | en_US |
dc.subject.emtree | Beta glucan | en_US |
dc.subject.emtree | Fungal DNA | en_US |
dc.subject.emtree | Galactomannan | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | DNA | en_US |
dc.subject.emtree | Galactomannan | en_US |
dc.subject.emtree | Mannan | en_US |
dc.subject.emtree | Adult | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Comparative study | en_US |
dc.subject.emtree | DNA extraction | en_US |
dc.subject.emtree | Hematologic malignancy | en_US |
dc.subject.emtree | High risk patient | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Human tissue | en_US |
dc.subject.emtree | Invasive aspergillosis | en_US |
dc.subject.emtree | Major clinical study | en_US |
dc.subject.emtree | Microscopy | en_US |
dc.subject.emtree | Predictive value | en_US |
dc.subject.emtree | Real time polymerase chain reaction | en_US |
dc.subject.emtree | Sensitivity and specificity | en_US |
dc.subject.emtree | Aspergillosis | en_US |
dc.subject.emtree | Aspergillus | en_US |
dc.subject.emtree | Hematologic disease | en_US |
dc.subject.emtree | Turkey (bird) | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Aspergillosis | en_US |
dc.subject.mesh | Aspergillus | en_US |
dc.subject.mesh | DNA | en_US |
dc.subject.mesh | Hematologic neoplasms | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mannans | en_US |
dc.subject.mesh | Turkey | en_US |
dc.subject.scopus | Invasive Aspergillosis; Mycoses; Systemic Mycosis | en_US |
dc.subject.wos | Medicine, general & internal | en_US |
dc.title | Comparison of galactomannan, beta-D-glucan, and Aspergillus DNA in sera of high-risk adult patients with hematological malignancies for the diagnosis of invasive aspergillosis | en_US |
dc.type | Article | |
dc.wos.quartile | Q3 | en_US |
dspace.entity.type | Publication | |
local.contributor.department | Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı | tr_TR |
local.contributor.department | Tıp Fakültesi/İç Hastalıkları Ana Bilim Dalı/Hematoloji Ünitesi | tr_TR |
local.contributor.department | Tıp Fakültesi/Tıbbi Mikrobiyoloji Ana Bilim Dalı | tr_TR |