İntraserebroventriküler olarak verilmiş Kolin'in dereceli kanatmanın yol açtığı plazma renin aktivitesi değişimine etkisi
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Date
1994
Authors
İşbil, Naciye
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Publisher
Uludağ Üniversitesi
Abstract
Çalışmamızda sıçanlara intraserebroventriküler (ICV) olarak verilen kolinin, dereceli kanatmanın yol açtığı kan basmcı ve plazma renin aktivitesi (PRA) değişimleri üzerine etkisi ve bu etkiye aracılık eden mekanizmalar incelendi. Ayrıca ICV fizostigminin de, dereceli kanatılan sıçanlarda kan basmcı ve PRA değişimleri üzerine etkisi araştırıldı. Deneylerde kullanılan dişi Wistar albino sıçanların sol karotis arterine kateter ve ICV enjeksiyonlar için yan ventriküle kanül yerleştirildi. ICV enjeksiyonlar yapıldıktan beş dakika sonra başlamak üzere beşer dakika aralıklarla, beş defa kan (0,5 ml/100 g vücut ağırlığı) alındı. Alınan her kan örneğinde PRA ölçülerek bir önceki kanatmanın PRA üzerine etkisi incelendi. İşlemler öncesinde, sırasında ve sonrasında kan basınçları belirlendi. ICV yolla verilen üç ayrı doz kolin (50,100,150 pg), dereceli kanatmanın kan basıncında neden olduğu düşmeyi azaltırken, PRA'ndeki artışı baskıladı. Nikotinik reseptör antagonist! mekamilamin ve muskarinik reseptör antagonisti atropin, kolinin kan basına üzerindeki etkisini önledi. Mekamilamin kolinin PRA üzerindeki etkisini önlerken atropin ise etkisizdi. Vazopressinin pressor etkisine spesifik antagonisti, intraarterial yolla verildiğinde kolinin hem kan basıncına hem de PRA üzerine olan etkisini bloke etti. Asetilkolinesteraz inhibitörü olan fizostigmin intraserebroventriküler yolla verildiğinde, dereceli kanatmanın yol açtığı PRA değişimi ve kan basmcı üzerinde kolin ile aynı yönde etki oluşturdu. -1-Bulgularımız, intraserebroventriküler yolla verilen kolinin dereceli kanatmanın kan basıncında oluşturduğu düşüşü azalttığını ve PRA'ndeki artışı baskıladığını göstermektedir. Kolinin kan basıncına etkisi merkezi nikotinik ve muskarinik reseptörlerin uyarılması sonucu ortaya çıkmaktadır. Kolinin PRA üzerindeki etkisi ise nikotinik reseptörlerin uyarılması sonucu ortaya çıkmaktadır. Bu etkilere vazopressinerjik sistemin aracılık ettiği düşünülmektedir. Sonuç olarak, intraserebro ventriküler yolla uygulanan kolin ve fizostigminin kan basıncını yükseltici etkilerinde renin-anjiotensin sisteminin rolü olmadığı düşünülmektedir.
In our study, the influence of choline given intracerebroventricularly (ICV) on the changes related to blood pressure and plasma renin activity induced by intermittant hemorrhage and the mechanisms contributing to this influence have been examined. Also, the influence of ICV physostigmine on the changes related to the blood pressure and PRA in the rats vvith intermittant hemorrhage has been investigated. A catheter was inserted into the left carotis artery of the female Wistar albino rats and another cannula was placed into their right lateral ventricule for ICV injections. Five minutes after ICV injections were made, blood (0,5 mİ /100 g body weight) was taken in five minutes intervals for five times. The influence of each hemorrhage on PRA was examined by measuring PRA in each blood sample taken. Blood pressures were determined before, during and after the processes. While three different doses of choline (50,100,150 //g) given ICV decreased the fail in the blood pressure caused by intermittant hemorrhage, also blocked the increase in PRA. Nicotinic receptor antagonist mecamylamine and muscarinic receptor antagonist atropine blocked the influence of choline on the blood pressure. Whilst mecamylamine stopped the influence of choline on the PRA, atropine did not affected PRA. VVhen the specific antagonist was given intraarterially to block of vasopressine as a pressor, it prevented the influence of choline on both blood pressure and also PRA. When physostigmine, which is an inhibitor of acetylcholinesterase, was given intracerebroventricularly, it had an effect similar to the influence of choline on the blood pressure and PRA caused by intennittant hemorrhage. Our findings represent that the choline given intracerebroventricularly decreases the fail in the blood pressure caused by intennittant hemorrhage and inhibits the increase in PRA. The blood pressure influence of choline has thought to a result of the stimulation of central nicotinic and muscarinic receptors, The PRA influence of choline have emerged as a result of the stimulation of nicotinic receptors. Vasopressinergic system also contributes to this influences. Consequently, it is also considered that renin-angiotensine system has no role in the influences of choline and physostigmine, when they are applied intracerebroventricularly, leading to on increase in blood pressure.
In our study, the influence of choline given intracerebroventricularly (ICV) on the changes related to blood pressure and plasma renin activity induced by intermittant hemorrhage and the mechanisms contributing to this influence have been examined. Also, the influence of ICV physostigmine on the changes related to the blood pressure and PRA in the rats vvith intermittant hemorrhage has been investigated. A catheter was inserted into the left carotis artery of the female Wistar albino rats and another cannula was placed into their right lateral ventricule for ICV injections. Five minutes after ICV injections were made, blood (0,5 mİ /100 g body weight) was taken in five minutes intervals for five times. The influence of each hemorrhage on PRA was examined by measuring PRA in each blood sample taken. Blood pressures were determined before, during and after the processes. While three different doses of choline (50,100,150 //g) given ICV decreased the fail in the blood pressure caused by intermittant hemorrhage, also blocked the increase in PRA. Nicotinic receptor antagonist mecamylamine and muscarinic receptor antagonist atropine blocked the influence of choline on the blood pressure. Whilst mecamylamine stopped the influence of choline on the PRA, atropine did not affected PRA. VVhen the specific antagonist was given intraarterially to block of vasopressine as a pressor, it prevented the influence of choline on both blood pressure and also PRA. When physostigmine, which is an inhibitor of acetylcholinesterase, was given intracerebroventricularly, it had an effect similar to the influence of choline on the blood pressure and PRA caused by intennittant hemorrhage. Our findings represent that the choline given intracerebroventricularly decreases the fail in the blood pressure caused by intennittant hemorrhage and inhibits the increase in PRA. The blood pressure influence of choline has thought to a result of the stimulation of central nicotinic and muscarinic receptors, The PRA influence of choline have emerged as a result of the stimulation of nicotinic receptors. Vasopressinergic system also contributes to this influences. Consequently, it is also considered that renin-angiotensine system has no role in the influences of choline and physostigmine, when they are applied intracerebroventricularly, leading to on increase in blood pressure.
Description
Keywords
Kolin, İntraserebroventriküler, Dereceli kanatma, Renin, Kan basıncı, Choline, Intracerebroventricular, Intennittant hemorrhage, Blood pressure
Citation
İşbil, N. (1994). İntraserebroventriküler olarak verilmiş Kolin'in dereceli kanatmanın yol açtığı plazma renin aktivitesi değişimine etkisi. Yayınlanmamış doktora tezi. Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü.