Apoptosis-inducing effect of a palladium(II) complex-[PdCl(terpy)](sac)center dot 2H2O] on ehrlich ascites carcinoma (EAC) in mice
Date
2016-04-28
Authors
İkitimur, Elif I. Armutak
Gürel, Ebru Gürevin
Yaylım, İlhan
İşbilen, Banu Başok
Şennazlı, Gülbin
Yüzbaşıoğlu, Gülay Öztürk
Sönmez, Kıvılcım
Çelik, Faruk
Küçükhüseyin, Özlem
Korkmaz, Gurbet
Journal Title
Journal ISSN
Volume Title
Publisher
Int Inst Anticancer Research
Abstract
Background/Aim: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)center dot 2H2O] (sac= saccharinate, and terpy= 2,2':6',2 ''- terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo. Materials and Methods: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3 beta, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis. Results: Expression of p53, PCNA, Bcl-2 was found decreased (p< 0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p< 0.001) in all groups. The survivalrelated markers did not show any statistical difference in complex groups. Conclusion: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel.
Description
Keywords
Research & experimental medicine, Pd(II) complex, EAC, Breast cancer, Apoptosis, In vivo, Breast-cancer cells, Nuclear antigen immunohistochemistry, Gene bax-alpha, Insulin-receptor substrate-1, Saccharinate complex, In-vitro, Tumor-growth, Death, Phosphorylation, Terpyridine
Citation
İkitimur, A. E. I. vd. (2016). "Apoptosis-inducing effect of a palladium(II) complex-[PdCl(terpy)](sac)center dot 2H2O] on ehrlich ascites carcinoma (EAC) in mice". In Vivo, 30(4), 457-464.