Peripheral GLP-1 gastroprotection against ethanol: The role of exendin, NO, CGRP, prostaglandins and blood flow
Date
2009-01-08
Authors
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Publisher
Elsevier
Abstract
The aim of this study was to investigate the effects of peripherally injected glucagon like peptide-1 (GLP-1) on ethanol-induced gastric mucosal damage and the mechanisms included in the effect.
Absolute ethanol was administered through an orogastric cannula right after the injection of GLP-1 (1, 10, 100, 1000 or 10,000 ng/kg; i.p.). The rats were decapitated an hour later, the stomachs removed and the gastric mucosal damage scored. 1000 ng GLP-1 inhibited gastric mucosal damage by 45% and 10,000 ng GLP-1 by 60%. The specific receptor antagonist exendin-(9-39) (2500 ng/kg; i.p.), calcitonin gene related peptide (CGRP) receptor antagonist CGRP-(8-37) (10 pg/kg; i.p.), nitric oxide (NO) synthase inhibitor L-NAME (30 mg/kg; s.c) and cyclooxygenase inhibitor indomethacin (5 mg/kg; i.p.) inhibited the preventive effect of GLP-1 on ethanol-induced gastric mucosal damage. GLP-1 also prevented the decrease in gastric mucosal blood flow caused by ethanol when administered at gastroprotective doses (1000 and 10,000 ng/kg: i.p.).
In conclusion, GLP-1 administered peripherally prevents the gastric mucosal damage caused by ethanol in rats. CGRR NO, prostaglandin and gastric mucosal blood flow are thought to play a role in this effect, mediated through receptors specific to GLP-1.
Description
Keywords
CGRP-(8-37), Exendin-(9-39), Gastric mucosal blood flow, Gastric mucosal damage, Glucagon like peptide-1, Indomethacin, l-NAME, Glucagon-like peptide-1, Gene-related peptide, Gastric somatostatin, Experimental-models, Rat, Receptor, Lesions, Amylin, Amide, Mechanisms, Endocrinology & metabolism, Physiology, Rattus
Citation
İşbil, N. B. vd. (2009). "Peripheral GLP-1 gastroprotection against ethanol: The role of exendin, NO, CGRP, prostaglandins and blood flow". Regulatory Peptides, 152(1-3), 22-27.