Using microwave irradiation in Marchi's method for demonstrating degenerated myelin
Date
2006
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
Conventional methods for histological preparation of degenerated myelin are time-consuming and difficult. The purpose of our study was to shorten the time required for the procedure and to obtain better quality results for light microscopic demonstration of degenerated myelin in the central and peripheral nervous systems by using microwave irradiation. Rat brain and sciatic nerve were used for the study. The middle cerebral artery was occluded and the sciatic nerve was cut to produce myelin degeneration. Marchi's method was used for staining degenerated myelin. Fixation for light microscopy that would take two days using the conventional procedure was completed in 16.5-18.5 min using microwave irradiation. While staining of degenerated myelin requires 10 days for the conventional Marchi method, we decreased it to 7 h for brain tissue and I h for sciatic nerve by using the microwave oven. Moreover, a better quality preparation was achieved in the groups stained under microwave irradiation than those prepared by the conventional method.
Description
Keywords
Biotechnology & applied microbiology, Cell biology, Myelin degeneration, Microwave irradiation, Marchi's method, Brain, Tissue, Rats, Oven, Fixation, Cerebral-artery occlusion
Citation
Avcı, B. vd. (2006). ''Using microwave irradiation in Marchi's method for demonstrating degenerated myelin''. Biotechnic and Histochemistry, 81(2-3), 63-69.