Using microwave irradiation in Marchi's method for demonstrating degenerated myelin
dc.contributor.buuauthor | Avcı, Berrin | |
dc.contributor.buuauthor | Kahveci, Nevzat | |
dc.contributor.buuauthor | Kahveci, Zeynep | |
dc.contributor.buuauthor | Sırmalı, S. A. | |
dc.contributor.department | Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Bölümü. | tr_TR |
dc.contributor.orcid | 0000-0003-0841-8201 | tr_TR |
dc.contributor.researcherid | ABE-6685-2020 | tr_TR |
dc.contributor.researcherid | AAG-7070-2021 | tr_TR |
dc.contributor.scopusid | 6603017388 | tr_TR |
dc.contributor.scopusid | 6602597846 | tr_TR |
dc.contributor.scopusid | 6603395784 | tr_TR |
dc.contributor.scopusid | 14069270300 | tr_TR |
dc.date.accessioned | 2021-12-30T05:49:42Z | |
dc.date.available | 2021-12-30T05:49:42Z | |
dc.date.issued | 2006 | |
dc.description.abstract | Conventional methods for histological preparation of degenerated myelin are time-consuming and difficult. The purpose of our study was to shorten the time required for the procedure and to obtain better quality results for light microscopic demonstration of degenerated myelin in the central and peripheral nervous systems by using microwave irradiation. Rat brain and sciatic nerve were used for the study. The middle cerebral artery was occluded and the sciatic nerve was cut to produce myelin degeneration. Marchi's method was used for staining degenerated myelin. Fixation for light microscopy that would take two days using the conventional procedure was completed in 16.5-18.5 min using microwave irradiation. While staining of degenerated myelin requires 10 days for the conventional Marchi method, we decreased it to 7 h for brain tissue and I h for sciatic nerve by using the microwave oven. Moreover, a better quality preparation was achieved in the groups stained under microwave irradiation than those prepared by the conventional method. | en_US |
dc.identifier.citation | Avcı, B. vd. (2006). ''Using microwave irradiation in Marchi's method for demonstrating degenerated myelin''. Biotechnic and Histochemistry, 81(2-3), 63-69. | en_US |
dc.identifier.endpage | 69 | tr_TR |
dc.identifier.issn | 1052-0295 | |
dc.identifier.issn | 1473-7760 | |
dc.identifier.issue | 2-3 | tr_TR |
dc.identifier.pubmed | 16908430 | tr_TR |
dc.identifier.scopus | 2-s2.0-33747343227 | tr_TR |
dc.identifier.startpage | 63 | tr_TR |
dc.identifier.uri | https://doi.org/10.1080/10520290600783044 | |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1080/10520290600783044 | |
dc.identifier.uri | http://hdl.handle.net/11452/23743 | |
dc.identifier.volume | 81 | tr_TR |
dc.identifier.wos | 000240796000002 | tr_TR |
dc.indexed.pubmed | Pubmed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Taylor & Francis | en_US |
dc.relation.journal | Biotechnic and Histochemistry | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Biotechnology & applied microbiology | en_US |
dc.subject | Cell biology | en_US |
dc.subject | Myelin degeneration | en_US |
dc.subject | Microwave irradiation | en_US |
dc.subject | Marchi's method | en_US |
dc.subject | Brain | en_US |
dc.subject | Tissue | en_US |
dc.subject | Rats | en_US |
dc.subject | Oven | en_US |
dc.subject | Fixation | en_US |
dc.subject | Cerebral-artery occlusion | en_US |
dc.subject.emtree | Tissue preparation | en_US |
dc.subject.emtree | Tissue fixation | en_US |
dc.subject.emtree | Time | en_US |
dc.subject.emtree | Staining | en_US |
dc.subject.emtree | Sciatic nerve | en_US |
dc.subject.emtree | Rat | en_US |
dc.subject.emtree | Quality control | en_US |
dc.subject.emtree | Animal tissue | en_US |
dc.subject.emtree | Peripheral nervous system | en_US |
dc.subject.emtree | Nonhuman | en_US |
dc.subject.emtree | Nerve degeneration | en_US |
dc.subject.emtree | Middle cerebral artery occlusion | en_US |
dc.subject.emtree | Microwave irradiation | en_US |
dc.subject.emtree | Microscopy | en_US |
dc.subject.emtree | Intermethod comparison | en_US |
dc.subject.emtree | Demyelinating disease | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Central nervous system | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.mesh | Staining and labeling | en_US |
dc.subject.mesh | Sciatic nerve | en_US |
dc.subject.mesh | Rats, sprague-dawley | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Myelin sheath | en_US |
dc.subject.mesh | Microwaves | en_US |
dc.subject.mesh | Brain | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.scopus | Fixatives; Tissue Fixation; Immunohistochemistry | en_US |
dc.subject.wos | Biotechnology & applied microbiology | en_US |
dc.subject.wos | Cell biology | en_US |
dc.title | Using microwave irradiation in Marchi's method for demonstrating degenerated myelin | en_US |
dc.type | Article | |
dc.wos.quartile | Q4 | en_US |
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