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Title: Hemorrhage activates proopiomelanocortin neurons in the rat hypothalamus
Authors: Levendusky, M. C.
Resch, Garth. E.
Veno, Patricia A.
Millington, W. R.
Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Klinik Farmakoloji Anabilim Dalı.
Göktalay, Gökhan
Çavun, Sinan
Keywords: Neurosciences & neurology
Blood pressurse
Arcuate nucleus
Acute hypovolemia
Opiate antagonists
Decompensatory phase
Periaqueductal gray
Ventrolateral medulla
Paraventricular nucleus
Fos expression
Opioid receptors
Issue Date: 27-Jan-2006
Publisher: Elsevier
Citation: Göktalay, G. vd. (2006). ''Hemorrhage activates proopiomelanocortin neurons in the rat hypothalamus''. Brain Research, 1070(1), 45-55.
Abstract: Severe blood loss lowers arterial pressure through a central mechanism that is thought to include opioid neurons. In this study, we investigated whether hemorrhage activates proopiomelanocortin (POMC) neurons by measuring Fos immunoreactivity and POMC mRNA levels in the medial basal hypothalamus. Hemorrhage (2.2 ml/100 g body weight over 20 min) increased the number of Fos immunoreactive neurons throughout the rostral-caudal extent of the arcuate nucleus, the retrochiasmatic area and the peri-arcuate region lateral to the arcuate nucleus where POMC neurons are located. Double label immunohistochemistry revealed that hemorrhage increased Fos expression by beta-endorphin immunoreactive neurons significantly. The proportion of beta-endorphin immunoreactive neurons that expressed Fos immunoreactivity increased approximately four-fold, from 11.7 +/- 1.4% in sham-operated control animals to 42.0 +/- 5.2% in hemorrhaged animals. Hemorrhage also increased POMC mRNA levels in the medial basal hypothalamus significantly, consistent with the hypothesis that blood loss activates POMC neurons. To test whether activation of arcuate neurons contributes to the fall in arterial pressure evoked by hemorrhage, we inhibited neuronal activity in the caudal arcuate nucleus by microinjecting the local anesthetic lidocaine (2%; 0.1 or 0.3 mu l) bilaterally 2 min before hemorrhage was initiated. Lidocaine injection inhibited hemorrhagic hypotension and bradycardia significantly although it did not influence arterial pressure or heart rate in non-hemorrhaged rats. These results demonstrate that hemorrhage activates POMC neurons and provide evidence that activation of neurons in the arcuate nucleus plays an important role in the hemodynamic response to hemorrhage.
ISSN: 0006-8993
Appears in Collections:Scopus
Web of Science

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