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The identification of matrix metalloproteinases and their tissue inhibitors in broiler chickens by immunohistochemistry

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Özyiğit, Musa Özgür
Kahraman, Müjdat Mufit
Sönmez, Gürsel

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Taylor & Francis

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Abstract

The aim of this study was to investigate the distribution of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 using immunohistochemistry in the ascites syndrome of broiler chickens in a salt-induced experimental model. The presence of the enzymes in the lung, heart, liver, kidney and brain was evaluated semi-quantitatively with the streptavidin - biotin - peroxidase (Strep-ABC) method using commercially available primary monoclonal antibodies. Immunostaining of MMP-2 and MMP-9 was more intense and extensive in ascitic broilers than in the controls, although a decrease was seen with increasing age both in normal and ascitic chickens. The presence of MMP-9 enzyme was negatively correlated with the presence of TIMP-1 enzyme. It is suggested that MMP-2 and MMP-9 enzymes might play a role in the permeability increase of vessel walls by the destruction of the basement membranes in the salt-induced experimental ascites syndrome in broiler chickens.

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Drinking-water, Brain, Heart-failure, Lung edema, Expression, Ascites, Reperfusion, Lesions, Injury, Sites, Veterinary sciences

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Özyiğit, M. O. vd. (2005). "The identification of matrix metalloproteinases and their tissue inhibitors in broiler chickens by immunohistochemistry". Avian Pathology, 34(6), 509-516.

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