The identification of matrix metalloproteinases and their tissue inhibitors in broiler chickens by immunohistochemistry

Date

2005-12

Authors

Journal Title

Journal ISSN

Volume Title

Publisher

Taylor & Francis

Abstract

The aim of this study was to investigate the distribution of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 using immunohistochemistry in the ascites syndrome of broiler chickens in a salt-induced experimental model. The presence of the enzymes in the lung, heart, liver, kidney and brain was evaluated semi-quantitatively with the streptavidin - biotin - peroxidase (Strep-ABC) method using commercially available primary monoclonal antibodies. Immunostaining of MMP-2 and MMP-9 was more intense and extensive in ascitic broilers than in the controls, although a decrease was seen with increasing age both in normal and ascitic chickens. The presence of MMP-9 enzyme was negatively correlated with the presence of TIMP-1 enzyme. It is suggested that MMP-2 and MMP-9 enzymes might play a role in the permeability increase of vessel walls by the destruction of the basement membranes in the salt-induced experimental ascites syndrome in broiler chickens.

Description

Keywords

Drinking-water, Brain, Heart-failure, Lung edema, Expression, Ascites, Reperfusion, Lesions, Injury, Sites, Veterinary sciences

Citation

Özyiğit, M. O. vd. (2005). "The identification of matrix metalloproteinases and their tissue inhibitors in broiler chickens by immunohistochemistry". Avian Pathology, 34(6), 509-516.