Publication:
Simultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay

dc.contributor.authorRumbeiha, Wilson K.
dc.contributor.authorEnsley, Steve
dc.contributor.authorOlsen, Chris
dc.contributor.authorSchrunk, Dwayne E.
dc.contributor.buuauthorOruç, Hasan Hüseyin
dc.contributor.departmentVeterinerlik Fakültesi
dc.contributor.departmentFarmakoloji ve Toksikoloji Ana Bilim Dalı
dc.contributor.orcid0000-0002-5399-2395
dc.contributor.researcheridAAI-2212-2021
dc.contributor.scopusid55944769400
dc.date.accessioned2024-04-01T07:42:54Z
dc.date.available2024-04-01T07:42:54Z
dc.date.issued2013
dc.description.abstractThe objectives of this study were 1) to determine whether a biochip array-based immunoassay could be used to detect 6 different group antimicrobials in porcine oral fluids collected under experimental conditions; 2) to determine the feasibility of antimicrobial detection in clean versus dirty oral fluid samples; and 3) to determine if the assay could be used to detect chlortetracycline in oral fluids of swine ingesting a routine diet containing chlortetracycline. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different group six antimicrobials in milk, urine, meat, honey, and feed. The assay had not been investigated for potential use in oral fluids. Following evaluation of different extraction procedures, a centrifugation method was chosen. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in both clean and dirty oral fluid samples. However, tetracycline was not well recovered in dirty samples. Chlortetracycline from tetracycline group was detected in all oral fluid samples collected from the field at concentrations ranging from 176.0-698.6 ppb. In conclusion, this assay can detect all target antibacterials from different groups in clean and dirty oral fluid samples with the exception of tetracycline which was not well-recovered from in dirty samples.
dc.description.sponsorshipMinistry of National Education - Turkey
dc.identifier.citationOruç, H. H. (2013). “Simultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay”. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 407-412.
dc.identifier.endpage412
dc.identifier.issn1300-6045
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84875039200
dc.identifier.startpage407
dc.identifier.urihttps://doi.org/10.9775/kvfd.2012.7905
dc.identifier.urihttps://hdl.handle.net/11452/40881
dc.identifier.volume19
dc.identifier.wos000321750600007
dc.indexed.scopusScopus
dc.indexed.trdizinTRDizin
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherKafkas Üniversitesi
dc.relation.collaborationYurt dışı
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectVeterinary sciences
dc.subjectAntimicrobials
dc.subjectBiochip array-based immunoassay
dc.subjectOral fluid
dc.subjectSwine
dc.subjectSaliva
dc.subject.scopusMouth; Classical Swine Fever; Swine Diseases
dc.subject.wosVeterinary sciences
dc.titleSimultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay
dc.typeArticle
dc.wos.quartileN/A
dspace.entity.typePublication
local.contributor.departmentVeterinerlik Fakültesi/Farmakoloji ve Toksikoloji Ana Bilim Dalı
local.indexed.atTrDizin
local.indexed.atWOS
local.indexed.atScopus

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